Inhibitors of HIV-1 Reverse Transcriptase—Associated
... chemotypes. In the same context however, structural similarity with human RNase H1 raises concerns for potential off-target activity. Second was our development of a robust fluorescence-based assay, adaptable to robotic high throughput screening [23±25]. As of mid-2012, numerous small molecule RNHIs ...
... chemotypes. In the same context however, structural similarity with human RNase H1 raises concerns for potential off-target activity. Second was our development of a robust fluorescence-based assay, adaptable to robotic high throughput screening [23±25]. As of mid-2012, numerous small molecule RNHIs ...
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... not with genetic divergence [28,29]. These correlations are inferred to be due to an indirect effect of recombination due to the interaction between selection and linkage and their strength can be used to make inferences about the pervasiveness of natural selection. Positive selection on favourable ...
... not with genetic divergence [28,29]. These correlations are inferred to be due to an indirect effect of recombination due to the interaction between selection and linkage and their strength can be used to make inferences about the pervasiveness of natural selection. Positive selection on favourable ...
Quantitative Analysis of the Kinetics of End
... addition, the binding of ATP or ATPgS to individual ®lament subunits can stimulate NTP hydrolysis in neighboring subunits (Lee & Cox, 1990; Menge & Bryant, 1988). These interactions tend to maintain the entire ®lament in an extended active state. However, the ATP hydrolytic cycles of adjacent monome ...
... addition, the binding of ATP or ATPgS to individual ®lament subunits can stimulate NTP hydrolysis in neighboring subunits (Lee & Cox, 1990; Menge & Bryant, 1988). These interactions tend to maintain the entire ®lament in an extended active state. However, the ATP hydrolytic cycles of adjacent monome ...
DISSOLUTION METHOD DEVELOPMENT OF LAMIVUDINE, NEVIRAPINE AND STAVUDINE IN TABLETS BY UPLC
... Accurately weighed and transferred 16.8, 22.4 and 44 mg of lamivudine, nevirapine and stavudine, respectively, into a 100 mL volumetric flask, dissolved and diluted with the diluent. A 10 mL of this solution was diluted into 100 mL with dissolution media. RESULTS AND DISCUSSIO ...
... Accurately weighed and transferred 16.8, 22.4 and 44 mg of lamivudine, nevirapine and stavudine, respectively, into a 100 mL volumetric flask, dissolved and diluted with the diluent. A 10 mL of this solution was diluted into 100 mL with dissolution media. RESULTS AND DISCUSSIO ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
Updated slides on graph algorithms for DNA sequencing
... Eulerian path if and only if it contains two (complementary) semi-balanced vertices and all other vertices are balanced. ...
... Eulerian path if and only if it contains two (complementary) semi-balanced vertices and all other vertices are balanced. ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... In this study, we attempted to identify the amino acid residues responsible for acyl-CoA specificity in the acyltransferase family through structure-based mutagenesis studies on rat CrAT and COT proteins. As a result, we identified an amino acid residue (Met564 in rat CrAT) that is critical to fatty ...
... In this study, we attempted to identify the amino acid residues responsible for acyl-CoA specificity in the acyltransferase family through structure-based mutagenesis studies on rat CrAT and COT proteins. As a result, we identified an amino acid residue (Met564 in rat CrAT) that is critical to fatty ...
New Host Plants of Erwinia amylovora in Bulgaria
... profiles not found in previous studies, but all strains possessed the properties described for E. amylovora (Holt et al., 1994; Jones and Geider, 2001). The identification BIOLOG system is based on the utilization of 95 carbon sources in a microtiter plate. Cells were grown at 30 ∞C for 24 h on BUG ...
... profiles not found in previous studies, but all strains possessed the properties described for E. amylovora (Holt et al., 1994; Jones and Geider, 2001). The identification BIOLOG system is based on the utilization of 95 carbon sources in a microtiter plate. Cells were grown at 30 ∞C for 24 h on BUG ...
Potato Genome Sequencing Consortium: Final
... has four, often highly distinct copies of each of these genes. An important aspect of the PGSC’s work was the use of a ‘doubled monoploid’ (DM) clone of the Phureja group of potatoes which has two identical copies of each gene. This clone was made some time ago in the USA by making a ‘monoploid’ wit ...
... has four, often highly distinct copies of each of these genes. An important aspect of the PGSC’s work was the use of a ‘doubled monoploid’ (DM) clone of the Phureja group of potatoes which has two identical copies of each gene. This clone was made some time ago in the USA by making a ‘monoploid’ wit ...
KingFisher Pure DNA Plant Kit
... The KingFisher magnetic particle processors are designed for the automated transfer and processing of magnetic particles in microplate format. The patented technology of the Thermo Scientific™ KingFisher™ systems is based on the use of magnetic rods covered with a disposable, specially designed tip ...
... The KingFisher magnetic particle processors are designed for the automated transfer and processing of magnetic particles in microplate format. The patented technology of the Thermo Scientific™ KingFisher™ systems is based on the use of magnetic rods covered with a disposable, specially designed tip ...
Dynamic Changes in Aromatic Hydrocarbon Associated Catabolic
... Results from this work also indicate that more bcr copies were detected overall within the benzoate microcosms compared to the toluene microcosms. This could reflect the differences in the types of microorganisms present in the two inocula. For example microorganisms present in the river sediment us ...
... Results from this work also indicate that more bcr copies were detected overall within the benzoate microcosms compared to the toluene microcosms. This could reflect the differences in the types of microorganisms present in the two inocula. For example microorganisms present in the river sediment us ...
Comparison of different PCR tests for detecting Shiga toxin
... the need to find a single specific DNA sequence to identify EHEC O157. Moreover, one of the major drawbacks of the published PCR-based approaches for detection of STEC O157 is lack of internal amplification control (IAC) which is required in order to monitor false negatives that may be caused by PCR ...
... the need to find a single specific DNA sequence to identify EHEC O157. Moreover, one of the major drawbacks of the published PCR-based approaches for detection of STEC O157 is lack of internal amplification control (IAC) which is required in order to monitor false negatives that may be caused by PCR ...
Genome-wide analysis by SNP Array
... from one parent’s chromosome with silencing of the other parent’s chromosome. In the case of PWS, the paternal region is active, so that deletion or failure of inheritance of the paternal region causes the syndrome. PWS is characterised by mental retardation, hypogonadism, hypotonia, obesity, charac ...
... from one parent’s chromosome with silencing of the other parent’s chromosome. In the case of PWS, the paternal region is active, so that deletion or failure of inheritance of the paternal region causes the syndrome. PWS is characterised by mental retardation, hypogonadism, hypotonia, obesity, charac ...
Sequence variation in the dihydrofolate reductase
... Dihydrofolate reductase-thymidylate synthase (DHFR-TS ) and trypanothione reductase (TR ) are important enzymes for the metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited informat ...
... Dihydrofolate reductase-thymidylate synthase (DHFR-TS ) and trypanothione reductase (TR ) are important enzymes for the metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited informat ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... truncation mutants by using mini-transposon mediated random insertional mutagenesis approach. To confirm that the observed sporulation-temperature sensitive phenotype is caused by the transposon, a genetic outcross assay was carried out, and to determine the exact position of transposon integration ...
... truncation mutants by using mini-transposon mediated random insertional mutagenesis approach. To confirm that the observed sporulation-temperature sensitive phenotype is caused by the transposon, a genetic outcross assay was carried out, and to determine the exact position of transposon integration ...
hag expression in Bacillus subtilis is both negatively
... 200 mM of each dNTP. In general, the PCR was performed for 30 cycles with an initial denaturation step at 95 uC for 5 min and a final extension step at 72 uC for 10 min. The temperature and time for the denaturation, annealing and extension steps varied depending on the form of DNA, the Tm of the pr ...
... 200 mM of each dNTP. In general, the PCR was performed for 30 cycles with an initial denaturation step at 95 uC for 5 min and a final extension step at 72 uC for 10 min. The temperature and time for the denaturation, annealing and extension steps varied depending on the form of DNA, the Tm of the pr ...
Chromosomes Carrying Meiotic Avoidance Loci
... close to sexually programmed cells. Embryo and endosperm formation are both fertilization independent (autonomous) in apomictic Hieracium subgenus Pilosella, but in Pennisetum, fertilization is required for endosperm formation. A single dominant locus termed the Apospory-Specific Genomic Region (ASG ...
... close to sexually programmed cells. Embryo and endosperm formation are both fertilization independent (autonomous) in apomictic Hieracium subgenus Pilosella, but in Pennisetum, fertilization is required for endosperm formation. A single dominant locus termed the Apospory-Specific Genomic Region (ASG ...
The effecT of chlorinaTion of nucleoTide bases on The
... Recent studies on Escherichia coli bacteria cultivation, in which DNA thymine was replaced with 5-chlorouracil have refreshed the problem of understanding the changes to physical properties of DNA monomers resultant from chemical modifications. These studies have shown that the replacement did not a ...
... Recent studies on Escherichia coli bacteria cultivation, in which DNA thymine was replaced with 5-chlorouracil have refreshed the problem of understanding the changes to physical properties of DNA monomers resultant from chemical modifications. These studies have shown that the replacement did not a ...
New peptide and gene coding for same
... Figure 5 represents a sequence of a cDNA fragment cloned into plasmid phANP82 including a 5' noncoding region, a coding region corresponding to the base sequence set forth in Figure 4, and a 3' noncoding region; Figure 6 represents a construction process of plasmid pS220 from a plasmid pS20 and a DN ...
... Figure 5 represents a sequence of a cDNA fragment cloned into plasmid phANP82 including a 5' noncoding region, a coding region corresponding to the base sequence set forth in Figure 4, and a 3' noncoding region; Figure 6 represents a construction process of plasmid pS220 from a plasmid pS20 and a DN ...
Getting Started With Gel Electrophoresis
... gel because long agarose molecules hydrogen bond together, creating a large, threedimensional network trapping the water. This gel can then be placed in an electrophoresis chamber, which along with a power supply, creates an electrical field through the gel. When a charged molecule is placed in this ...
... gel because long agarose molecules hydrogen bond together, creating a large, threedimensional network trapping the water. This gel can then be placed in an electrophoresis chamber, which along with a power supply, creates an electrical field through the gel. When a charged molecule is placed in this ...
Statistical Analysis of Amino Acid Patterns in Transmembrane
... were counted. Raw counts are not very informative, since the main factor determining the gross number of occurrences of a pair in a set of sequences is the relative frequency of its residues. The Leu-Leu pairs at all separations (LLk), for instance, are the most numerous among the pairs in the datab ...
... were counted. Raw counts are not very informative, since the main factor determining the gross number of occurrences of a pair in a set of sequences is the relative frequency of its residues. The Leu-Leu pairs at all separations (LLk), for instance, are the most numerous among the pairs in the datab ...
Role of the p21 Protein in the Fanconi Anemia Pathway
... • Major target of the tumor suppressor transcription factor p53 Figure 8.4 The Biology of Cancer (© Garland Science 2007) ...
... • Major target of the tumor suppressor transcription factor p53 Figure 8.4 The Biology of Cancer (© Garland Science 2007) ...
Identification and isolation of active N2O reducers in rice paddy soil
... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).