Selection of Suitable Endogenous Reference Genes for Relative
... inserts. To accelerate the application of transformation in sugarcane, it is necessary and urgent to identify endogenous reference genes for transgenic sugarcane detection. Different molecular techniques such as Southern blotting [10], multiplex probe amplification and hybridization and microarray a ...
... inserts. To accelerate the application of transformation in sugarcane, it is necessary and urgent to identify endogenous reference genes for transgenic sugarcane detection. Different molecular techniques such as Southern blotting [10], multiplex probe amplification and hybridization and microarray a ...
somatic hypermutation of the 5' noncoding region of the Frequent MARTINOrrI*t,
... well as rearranged BCL6 alleles as previously characterized by Southern blot analysis of genomic DNA (9). Karyotype information was available for 13 cases which carried normal or abnormal chromosomal band 3q27, the site of the BCL6 gene (4). BCL6 sequences representing exons 2-10 and spanning the en ...
... well as rearranged BCL6 alleles as previously characterized by Southern blot analysis of genomic DNA (9). Karyotype information was available for 13 cases which carried normal or abnormal chromosomal band 3q27, the site of the BCL6 gene (4). BCL6 sequences representing exons 2-10 and spanning the en ...
64$ CfE Higher Biology Unit 1: DNA and the
... • DNA takes the form of a double-stranded helix; • the two strands of DNA are held together by complementary base pairs; • DNA contains the four bases A, T, G and C which make up the genetic code. Learning Objectives By the end of this topic, you should be able to: • appreciate that DNA is found in ...
... • DNA takes the form of a double-stranded helix; • the two strands of DNA are held together by complementary base pairs; • DNA contains the four bases A, T, G and C which make up the genetic code. Learning Objectives By the end of this topic, you should be able to: • appreciate that DNA is found in ...
64$ CfE Higher Biology Unit 1: DNA and the
... • DNA takes the form of a double-stranded helix; • the two strands of DNA are held together by complementary base pairs; • DNA contains the four bases A, T, G and C which make up the genetic code. Learning Objectives By the end of this topic, you should be able to: • appreciate that DNA is found in ...
... • DNA takes the form of a double-stranded helix; • the two strands of DNA are held together by complementary base pairs; • DNA contains the four bases A, T, G and C which make up the genetic code. Learning Objectives By the end of this topic, you should be able to: • appreciate that DNA is found in ...
Prevention of DNA Rereplication Through a Meiotic Recombination
... were used to inoculate YPD liquid cultures. The overnight YPD cultures were then used to inoculate YPA (1% (w/v) yeast extract, 2% (w/v) peptone, 2% (w/v) potassium acetate) at an OD600 of ~0.2. Cells were incubated overnight, typically for 16 hours, and then resuspended at equivalent cell densi ...
... were used to inoculate YPD liquid cultures. The overnight YPD cultures were then used to inoculate YPA (1% (w/v) yeast extract, 2% (w/v) peptone, 2% (w/v) potassium acetate) at an OD600 of ~0.2. Cells were incubated overnight, typically for 16 hours, and then resuspended at equivalent cell densi ...
Exome sequencing as a tool for Mendelian disease gene discovery
... case–control design, although reduced costs of exome sequencing coupled with increasing access to large numbers of publically available control exomes should increase the popularity of this approach. A major challenge of relating rare variants to a trait is that even with very large sample sizes, th ...
... case–control design, although reduced costs of exome sequencing coupled with increasing access to large numbers of publically available control exomes should increase the popularity of this approach. A major challenge of relating rare variants to a trait is that even with very large sample sizes, th ...
Foundations in Microbiology
... Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education. ...
... Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education. ...
Identification of Genetic and Epigenetic Risk Factors for Psoriasis
... thesis examination committee, Dr. Anne Bowcock. It is difficult to overstate my gratitude to Anne. It was such a great honor and pleasant to work with Anne and during the past 6 years, she has been giving me enthusiastic supervision in ensuring my academic, professional, financial, and moral well-be ...
... thesis examination committee, Dr. Anne Bowcock. It is difficult to overstate my gratitude to Anne. It was such a great honor and pleasant to work with Anne and during the past 6 years, she has been giving me enthusiastic supervision in ensuring my academic, professional, financial, and moral well-be ...
Caspary T, Cleary MA, Baker CC, Guan XJ, Tilghman SM. Mol Cell Biol. 1998 Jun;18(6):3466-74. Multiple mechanisms of imprinting on distal mouse chromosome 7.
... congenic BTBR strain containing sequence from M. spretus at distal chromosome 7. We identified polymorphisms between alleles from the two species and developed RT-PCR assays to determine which parental allele was expressed in the offspring. For each assay, mixing controls were used to verify that th ...
... congenic BTBR strain containing sequence from M. spretus at distal chromosome 7. We identified polymorphisms between alleles from the two species and developed RT-PCR assays to determine which parental allele was expressed in the offspring. For each assay, mixing controls were used to verify that th ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Clostridium hiranonis sp. nov., a human intestinal bacterium with
... to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 were 31n1 and 31n9 mol %, respectively. The levels of DNA–DNA hybridization between strains TO-931T and HD-17 ...
... to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 were 31n1 and 31n9 mol %, respectively. The levels of DNA–DNA hybridization between strains TO-931T and HD-17 ...
Whole-transcriptome RNAseq analysis from minute amount of total
... OvationÕ RNA-Seq system, a single sample was processed using six different input amounts of total RNA in the cDNA synthesis step. The seven aliquots of total RNA [500, 100 (with technical replicates), 50, 10 ng, 500 pg, 50 pg] was treated by DNase and subjected to cDNA synthesis. Also, cDNA product ...
... OvationÕ RNA-Seq system, a single sample was processed using six different input amounts of total RNA in the cDNA synthesis step. The seven aliquots of total RNA [500, 100 (with technical replicates), 50, 10 ng, 500 pg, 50 pg] was treated by DNase and subjected to cDNA synthesis. Also, cDNA product ...
PDF
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
Classification of plant-pathogenic mycoplasma
... Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although se ...
... Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although se ...
Control Strategies for Synthetic Therapeutic Peptide APIs
... sequence of operations, the results may be variable. Assay by nitrogen content is also used routinely, most frequently determined from elemental analysis. Kjeldahl analysis or chromatographic methods with chemiluminescence nitrogen detection may also be used. Only peptides containing Trp, Tyr, or Ph ...
... sequence of operations, the results may be variable. Assay by nitrogen content is also used routinely, most frequently determined from elemental analysis. Kjeldahl analysis or chromatographic methods with chemiluminescence nitrogen detection may also be used. Only peptides containing Trp, Tyr, or Ph ...
Rapid communication: Nucleotide sequence of red seabream
... Japan). Double-stranded complementary DNA synthesis was carried out using a TimeSaver cDNA synthesis kit (Pharmacia Biotech, Uppsala, Sweden). The cDNA library was constructed with a Lamda gt10/EcoRI/ CIAP-treated vector (Stratagene, La Jolla, CA). To target β-actin cDNA from red seabream, primers f ...
... Japan). Double-stranded complementary DNA synthesis was carried out using a TimeSaver cDNA synthesis kit (Pharmacia Biotech, Uppsala, Sweden). The cDNA library was constructed with a Lamda gt10/EcoRI/ CIAP-treated vector (Stratagene, La Jolla, CA). To target β-actin cDNA from red seabream, primers f ...
On the maintenance of allozyme and inversion polymorphisms in
... Changes in environmentalternperaturewill intluence various factors, like egg productionandlan,al density.Therefore,intraspecificcompetitionfor food and differences in genotypes probably rate karyotypcs important fitness among and are developnrental in naluralD. melunogaslerpopulations.Such tcmpeÍatu ...
... Changes in environmentalternperaturewill intluence various factors, like egg productionandlan,al density.Therefore,intraspecificcompetitionfor food and differences in genotypes probably rate karyotypcs important fitness among and are developnrental in naluralD. melunogaslerpopulations.Such tcmpeÍatu ...
Epidemiology and control of fungally transmitted cereal viruses
... related to differences in isolate origin and ability to transmit virus. These DNA sequences were used to design sets of PCR primers for use in diagnostic tests. Two sets of primers were shown to specifically amplify sequences either from any Polymyxa isolate or only from temperate P. graminis isolat ...
... related to differences in isolate origin and ability to transmit virus. These DNA sequences were used to design sets of PCR primers for use in diagnostic tests. Two sets of primers were shown to specifically amplify sequences either from any Polymyxa isolate or only from temperate P. graminis isolat ...
1 - life.illinois.edu
... dnaA gene. How could you determine if his mutation was identical or different from your original dnaATS mutation? Answer: (If the mutation in your lab mate’s strain is at the same site as your mutation, you will not be able to obtain dnaA+ colonies when one strain is the donor and the other is the r ...
... dnaA gene. How could you determine if his mutation was identical or different from your original dnaATS mutation? Answer: (If the mutation in your lab mate’s strain is at the same site as your mutation, you will not be able to obtain dnaA+ colonies when one strain is the donor and the other is the r ...
Transgenerational epigenetics in the germline cycle
... with heritable epigenetic states (for example, [5,6]). However, DNA methylation and its heritable maintenance in those organisms where it occurs is influenced, if not regulated, by histone modifying activities [7]. As will be discussed in this review, recent studies in C. elegans have shown that def ...
... with heritable epigenetic states (for example, [5,6]). However, DNA methylation and its heritable maintenance in those organisms where it occurs is influenced, if not regulated, by histone modifying activities [7]. As will be discussed in this review, recent studies in C. elegans have shown that def ...
Metagenomic Analysis Using MEGAN4
... export all reads One can select a set of taxa and then use MEGAN to generate different types of charts ...
... export all reads One can select a set of taxa and then use MEGAN to generate different types of charts ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).