TD3 5.08 – PKS/NRPS pathways and mass
... PKS + everything + CD3-SAM -> same as above + 6Da for (CD3)2 instead of (CH3)2 Controls all look good - thioesters are stable to this method of analysis - resolution of technique at least 6 Da - technique also gives yields (relative peak intensities reflect relative product amounts) See reference: M ...
... PKS + everything + CD3-SAM -> same as above + 6Da for (CD3)2 instead of (CH3)2 Controls all look good - thioesters are stable to this method of analysis - resolution of technique at least 6 Da - technique also gives yields (relative peak intensities reflect relative product amounts) See reference: M ...
Nucleotide sequences from tomato leaf curl viruses from different
... in which extracts of naturally infected tomato leaves from many countries were allowed to react with panels of MAbs raised against the particles of two other WTGs, African cassava mosaic and Indian cassava mosaic viruses. The WTGs from tomato plants in different geographical regions proved to have c ...
... in which extracts of naturally infected tomato leaves from many countries were allowed to react with panels of MAbs raised against the particles of two other WTGs, African cassava mosaic and Indian cassava mosaic viruses. The WTGs from tomato plants in different geographical regions proved to have c ...
Review over DNA, RNA, proteins, viruses, bacteria, DNA technology
... ii. Replication requires DNA polymerase plus many other essential cellular enzymes, occurs bi-directionally, and differs in the production of the leading and lagging strands. ~The names of the steps and particular enzymes involved, beyond DNA polymerase, ligase, RNA polymerase, helicase, and topoiso ...
... ii. Replication requires DNA polymerase plus many other essential cellular enzymes, occurs bi-directionally, and differs in the production of the leading and lagging strands. ~The names of the steps and particular enzymes involved, beyond DNA polymerase, ligase, RNA polymerase, helicase, and topoiso ...
Preimplantation genetic diagnosis: State of the ART 2011
... aneuploidy screening, it is important to examine as many chromosomes as possible and up to 15 probes have been used (Baart et al. 2007). Since FISH is limited by the number of chromosomes that can be examined, many groups are replacing FISH with array-comparative genomic hybridisation (see below). M ...
... aneuploidy screening, it is important to examine as many chromosomes as possible and up to 15 probes have been used (Baart et al. 2007). Since FISH is limited by the number of chromosomes that can be examined, many groups are replacing FISH with array-comparative genomic hybridisation (see below). M ...
Bacterial disease resistance of transgenic hybrid poplar expressing
... used to verify that the ubi7-D4E1-nos expression cassette was present in the plant genome of transgenic clones. The ubiquitin promoter primer 5′-CTAATCAGGGAGTGTG-3′ and the D4E1-specific primer 5′-TAGCTCTCAATCTAACC -3′ were used to amplify a D4E1 gene-specific fragment of 349 bp. The PCR products we ...
... used to verify that the ubi7-D4E1-nos expression cassette was present in the plant genome of transgenic clones. The ubiquitin promoter primer 5′-CTAATCAGGGAGTGTG-3′ and the D4E1-specific primer 5′-TAGCTCTCAATCTAACC -3′ were used to amplify a D4E1 gene-specific fragment of 349 bp. The PCR products we ...
Genetics of Arabidopsis thaliana
... of 2001 are all over the media. All of these are problems that can be solved using the Polymerase Chain Reaction (PCR). PCR amplification is also widely used in plant biology; plant genotyping, gene mapping, diagnostics and diversity assessment all use the technique of PCR. In this lab, you will use ...
... of 2001 are all over the media. All of these are problems that can be solved using the Polymerase Chain Reaction (PCR). PCR amplification is also widely used in plant biology; plant genotyping, gene mapping, diagnostics and diversity assessment all use the technique of PCR. In this lab, you will use ...
Deconstructing the Genome: DNA at High Resolution
... that cut DNA, ligases that join two molecules together, and polymerases that synthesize new DNA strands. The most important uncatalyzed biochemical reaction is hybridization: the binding together of two DNA strands with complementary nucleotide sequences. Hybridization results from the natural prope ...
... that cut DNA, ligases that join two molecules together, and polymerases that synthesize new DNA strands. The most important uncatalyzed biochemical reaction is hybridization: the binding together of two DNA strands with complementary nucleotide sequences. Hybridization results from the natural prope ...
Electrophoresis Revised
... The lab will allow the students to be a forensic detective in their own classroom by analyzing DNA they found at the “crime scene” and comparing it to two suspects DNA. The students will be given a worksheet on Gel Electrophoresis and will also be given picture or what the guilty person’s DNA looks ...
... The lab will allow the students to be a forensic detective in their own classroom by analyzing DNA they found at the “crime scene” and comparing it to two suspects DNA. The students will be given a worksheet on Gel Electrophoresis and will also be given picture or what the guilty person’s DNA looks ...
Tertiary base pair interactions in slipped loop-DNA
... sequences can assume 'unusual' forms, such as Z-, H-forms, cruciforms, etc. (1,2). As a rule, such forms do not constitute the ground state (lowest energy) conformation, but they can be stabilized either by an appropriate solution environment (high ionic strength or low pH) or superhelical stress in ...
... sequences can assume 'unusual' forms, such as Z-, H-forms, cruciforms, etc. (1,2). As a rule, such forms do not constitute the ground state (lowest energy) conformation, but they can be stabilized either by an appropriate solution environment (high ionic strength or low pH) or superhelical stress in ...
Determination of obesity associated gene variants related
... and adolescents with ages ranging from 9 to 13 years old. This cohort is part of the ‘Healthy Growth Study’, a large-scale cross-sectional epidemiological study initiated in May 2007 and described previously (Moschonis et al., 2010). Swedish and Greek populations, despite having a common origin, dis ...
... and adolescents with ages ranging from 9 to 13 years old. This cohort is part of the ‘Healthy Growth Study’, a large-scale cross-sectional epidemiological study initiated in May 2007 and described previously (Moschonis et al., 2010). Swedish and Greek populations, despite having a common origin, dis ...
Fingerprinting the Fungal Community
... ecologists have come up with simpler, more rapid, methods of profiling fungal populations, collectively known as “community fingerprinting”. Although these do not produce the same high-quality sequence data as cloning-based approaches, they have a much higher sample throughput and are especially use ...
... ecologists have come up with simpler, more rapid, methods of profiling fungal populations, collectively known as “community fingerprinting”. Although these do not produce the same high-quality sequence data as cloning-based approaches, they have a much higher sample throughput and are especially use ...
word
... Genetic and epigenetic regulators of the germ line Specification of primordial germ cells (PGCs) in mammals occurs according to the 'stem cell model' in which pluripotent stem cells give rise to both the PGCs and somatic cells in response to signaling molecules. Expression of a transcriptional repre ...
... Genetic and epigenetic regulators of the germ line Specification of primordial germ cells (PGCs) in mammals occurs according to the 'stem cell model' in which pluripotent stem cells give rise to both the PGCs and somatic cells in response to signaling molecules. Expression of a transcriptional repre ...
computation for chIP-seq and rNA-seq studies
... 1×–10× coverage), and true ChIP-seq positive signals will be compressed along the chromosome because there is much less intergenic space in the smaller genomes. The strongest ChIP-enriched positions can have hundreds of overlapping reads for DNA-binding factors that are highly efficient targets for ...
... 1×–10× coverage), and true ChIP-seq positive signals will be compressed along the chromosome because there is much less intergenic space in the smaller genomes. The strongest ChIP-enriched positions can have hundreds of overlapping reads for DNA-binding factors that are highly efficient targets for ...
Identification and removal of colanic acid from plasmid DNA
... However, HPLC cannot detect these contaminating polysaccharides. The MWs of the residual polysaccharides vary over too wide a range. Therefore, not enough polysaccharide at any given MW is present in a measurable quantity for detection by HPLC. In addition, the wavelength used for HPLC would need to ...
... However, HPLC cannot detect these contaminating polysaccharides. The MWs of the residual polysaccharides vary over too wide a range. Therefore, not enough polysaccharide at any given MW is present in a measurable quantity for detection by HPLC. In addition, the wavelength used for HPLC would need to ...
Comparison of the separation of Candida albicans chromosome
... G by hybridization to the rRNA gene (Figure 2C) and band F was identified by hybridization to the HIS3 gene (Figure 2D). However, hybridization analysis of FIGE filters showed the hybridization of both the HIS3 gene (Figure 3B) and the rRNA gene (data not shown) to band F/G of Figure 3A. Hybridizati ...
... G by hybridization to the rRNA gene (Figure 2C) and band F was identified by hybridization to the HIS3 gene (Figure 2D). However, hybridization analysis of FIGE filters showed the hybridization of both the HIS3 gene (Figure 3B) and the rRNA gene (data not shown) to band F/G of Figure 3A. Hybridizati ...
Microarrays
... microarrays represent another alternative (e.g., xMAP technology from Luminex [15,16])). Even though, all these elements are essential building blocks of a good MDM, most challenging and most important task is to enable and demonstrate that the developed tool can perform as required in the scope of ...
... microarrays represent another alternative (e.g., xMAP technology from Luminex [15,16])). Even though, all these elements are essential building blocks of a good MDM, most challenging and most important task is to enable and demonstrate that the developed tool can perform as required in the scope of ...
Significant enhancement of fatty acid composition in seeds of the
... mutations over multiple generations at each of the three target sites in each of the three different FAD2 gene types present, respectively, in the A, B and C subgenomes of the allohexaploid genome. In the overview presented in Data Set S2, DNA sequences obtained by Sanger sequencing are provided for ...
... mutations over multiple generations at each of the three target sites in each of the three different FAD2 gene types present, respectively, in the A, B and C subgenomes of the allohexaploid genome. In the overview presented in Data Set S2, DNA sequences obtained by Sanger sequencing are provided for ...
Using comparative genomic hybridization to
... study, we propose the use of array-based comparative genomic hybridization (aCGH) in order to identify orthologous genes with high sequence divergence. Here we discuss experimental design, statistical power, success rate, sources of variation and potential confounding factors. We used a spotted PCR ...
... study, we propose the use of array-based comparative genomic hybridization (aCGH) in order to identify orthologous genes with high sequence divergence. Here we discuss experimental design, statistical power, success rate, sources of variation and potential confounding factors. We used a spotted PCR ...
physical maps
... Single nucleotide polymorphisms (SNPs) – 1/500 – 1/1000 bp across genome Simple sequence repeats (SSRs) – 1/20-1/40 kb across genome ...
... Single nucleotide polymorphisms (SNPs) – 1/500 – 1/1000 bp across genome Simple sequence repeats (SSRs) – 1/20-1/40 kb across genome ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).