Genetic Services-Intellectual Disability Project
... offers a number of advantages to FISH, principally in terms of cost and assay time, this may allow a broadening of referral criteria for patients with LD, and expand knowledge of subtelomeric abnormalities associated with this condition. In essence, the evaluation was aimed at determining whether ML ...
... offers a number of advantages to FISH, principally in terms of cost and assay time, this may allow a broadening of referral criteria for patients with LD, and expand knowledge of subtelomeric abnormalities associated with this condition. In essence, the evaluation was aimed at determining whether ML ...
BioBank cDNA Manual
... BioBank cDNA is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRNA transcripts in the specified tissue or cell line. BioBank cDNA is useful for expression profiling of newly identified genes, and also as a positive control for the real-t ...
... BioBank cDNA is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRNA transcripts in the specified tissue or cell line. BioBank cDNA is useful for expression profiling of newly identified genes, and also as a positive control for the real-t ...
A natural chimeric yeast containing genetic material from three species
... redissolved DNA was restricted with XbaI and the resulting DNA fragments were purified from agarose using Bio-Rad Prep-A-Gene purification matrix. These fragments were ligated into pUC19 vectors that had been linearized with XbaI and treated with calf intestine alkaline phosphatase. The resulting pl ...
... redissolved DNA was restricted with XbaI and the resulting DNA fragments were purified from agarose using Bio-Rad Prep-A-Gene purification matrix. These fragments were ligated into pUC19 vectors that had been linearized with XbaI and treated with calf intestine alkaline phosphatase. The resulting pl ...
Characterization of Two New Isolates of Mushroom
... as well as certain amino acids as sole carbon and energy sources. Disaccharides, complex carbohydrates, and gelatin were not used by any of the strains. Although similar, the metabolic capabilities of the strains were not identical. Several compounds were used by at least one but not by all four str ...
... as well as certain amino acids as sole carbon and energy sources. Disaccharides, complex carbohydrates, and gelatin were not used by any of the strains. Although similar, the metabolic capabilities of the strains were not identical. Several compounds were used by at least one but not by all four str ...
The serC-aroA operon of Escherichia coli
... Messing, 1983). The coding region of the aroA gene was identified by comparison of the translated DNA sequence with the N-terminal protein sequence of EPSP synthase. The start point of the aroA coding region is an ATG codon approx. 700 bp to the right of the Clal site and the gene runs left to right ...
... Messing, 1983). The coding region of the aroA gene was identified by comparison of the translated DNA sequence with the N-terminal protein sequence of EPSP synthase. The start point of the aroA coding region is an ATG codon approx. 700 bp to the right of the Clal site and the gene runs left to right ...
RHD - Labex
... 1961 Finn et al. demonstrated that the administration of anti-D accelerates the clearance of Rh+ RBC’s 1965 the first post partum administration of anti-D was given to a mother. 1977 the ante- post partum anti-D prophylaxis lead to almost 100% protection of the HDN due to anti-D ...
... 1961 Finn et al. demonstrated that the administration of anti-D accelerates the clearance of Rh+ RBC’s 1965 the first post partum administration of anti-D was given to a mother. 1977 the ante- post partum anti-D prophylaxis lead to almost 100% protection of the HDN due to anti-D ...
The promiscuous primase
... To date, crystal structures for the isolated catalytic subunit of archaeal primases from P. horikoshii and P. furiosus have been reported [7,8]. The crystallographic analysis showed that the catalytic subunit folds in a novel tertiary structure made up of two domains: a larger, mixed a/b domain, whe ...
... To date, crystal structures for the isolated catalytic subunit of archaeal primases from P. horikoshii and P. furiosus have been reported [7,8]. The crystallographic analysis showed that the catalytic subunit folds in a novel tertiary structure made up of two domains: a larger, mixed a/b domain, whe ...
A novel multifunctional O-methyltransferase implicated in a dual
... and 59-TCYTKYTSDGTYCTCTCTTT-39, 96-fold degeneracy; see the sequences with arrows below them in Fig. 1) based on a highly conserved C-terminal domain in plant CAOMT cDNAs were synthesized and used for PCR cloning of target DNA fragments according to the conditions described previously (22). A PCR pr ...
... and 59-TCYTKYTSDGTYCTCTCTTT-39, 96-fold degeneracy; see the sequences with arrows below them in Fig. 1) based on a highly conserved C-terminal domain in plant CAOMT cDNAs were synthesized and used for PCR cloning of target DNA fragments according to the conditions described previously (22). A PCR pr ...
Nucleosides, Nucleotides,Nucleic Acids
... Most of the RNA in a cell is ribosomal RNA Ribosomes are the site of protein synthesis. They are where translation of the mRNA sequence to an amino acid sequence occurs. Ribosomes are about two-thirds RNA and onethird protein. It is believed that the ribosomal RNA acts as a catalyst—a ribozyme. ...
... Most of the RNA in a cell is ribosomal RNA Ribosomes are the site of protein synthesis. They are where translation of the mRNA sequence to an amino acid sequence occurs. Ribosomes are about two-thirds RNA and onethird protein. It is believed that the ribosomal RNA acts as a catalyst—a ribozyme. ...
Pearson science 10 Teaching Program 3–4 weeks Chapter 1 DNA
... designing and constructing appropriate graphs to represent data, and analysing graphs for trends and patterns calculating means and ranges from data and considering the distribution of sets of quantitative data using spreadsheets to present data in tables and graphical forms and to carry out m ...
... designing and constructing appropriate graphs to represent data, and analysing graphs for trends and patterns calculating means and ranges from data and considering the distribution of sets of quantitative data using spreadsheets to present data in tables and graphical forms and to carry out m ...
Printable version - Chromosome 18 Registry and Research Society
... a position at 59 million base pairs of DNA to 72 million base pairs of DNA. The grey bars of varying lengths with letters next to them show the positions of particular genes. They are spread across the diagram for readability purposes only. This is because at this resolution if they were all in a ro ...
... a position at 59 million base pairs of DNA to 72 million base pairs of DNA. The grey bars of varying lengths with letters next to them show the positions of particular genes. They are spread across the diagram for readability purposes only. This is because at this resolution if they were all in a ro ...
Read the Nobel Lecture
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
CGH Microarray Solutions for Genome-Wide Genetic Analysis
... Agilent’s 2-color CGH array provides superior copy number data as opposed to 1-color because sample and reference DNA are directly compared. Agilent’s long 60-mer probes are more specific than probes that are shorter in length, allowing you to use fewer probes to make accurate calls. ...
... Agilent’s 2-color CGH array provides superior copy number data as opposed to 1-color because sample and reference DNA are directly compared. Agilent’s long 60-mer probes are more specific than probes that are shorter in length, allowing you to use fewer probes to make accurate calls. ...
Electron transfer from aromatic amino acids to guanine and adenine
... Because in most complexes G–X, DE is positive and the coupling is relatively small, the excess charge is mainly confined to G. Only in the edge dimers with X = Trp, Tyr and His, where DE < 0, the radical cation state is found on X. Since in the stacked dimers G–Trp, absolute values of DE and V are si ...
... Because in most complexes G–X, DE is positive and the coupling is relatively small, the excess charge is mainly confined to G. Only in the edge dimers with X = Trp, Tyr and His, where DE < 0, the radical cation state is found on X. Since in the stacked dimers G–Trp, absolute values of DE and V are si ...
A Tn 10-lacZ-kanR-URA3 Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes.
... DNA were obtained by transforming the target plasmid into a RecA- host such as NK5830 and preparing plasmid DNA from several transformants. The occasional dimeric transformant DNA is readily distinguished from monomeric transformant DNA by its gel mobility and can be discarded. Strains carrying both ...
... DNA were obtained by transforming the target plasmid into a RecA- host such as NK5830 and preparing plasmid DNA from several transformants. The occasional dimeric transformant DNA is readily distinguished from monomeric transformant DNA by its gel mobility and can be discarded. Strains carrying both ...
RealTime ready Cell Lysis Kit
... Using a Two-Step SYBR Green I Real-Time RT-PCR.” (2002). Biochem. 303: 95-98. 6 Bustin SA. et al. “The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.” (2009). Clin Chem. 55: 611-622. ...
... Using a Two-Step SYBR Green I Real-Time RT-PCR.” (2002). Biochem. 303: 95-98. 6 Bustin SA. et al. “The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.” (2009). Clin Chem. 55: 611-622. ...
Application of rpoB sequence similarity analysis, REP‐PCR and
... soil, seawater and sediment from two bays near the active volcano of Santorini at Nea Kameni island (Santorini, Greece, 2525¢N, 3625¢E), as described by Meintanis et al. (2006). The phylogenetic relatedness of the isolates is presented in Table 1. BOX- and REP-PCR analysis Bacteria were harvested ...
... soil, seawater and sediment from two bays near the active volcano of Santorini at Nea Kameni island (Santorini, Greece, 2525¢N, 3625¢E), as described by Meintanis et al. (2006). The phylogenetic relatedness of the isolates is presented in Table 1. BOX- and REP-PCR analysis Bacteria were harvested ...
v2 PEBiosystems News 2 01/00
... ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100-Avant Genetic Analyzers include patented technology licensed from Hitachi, Ltd. as part of a ...
... ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100-Avant Genetic Analyzers include patented technology licensed from Hitachi, Ltd. as part of a ...
Lack of homology between two haloacetate dehalogenase genes
... acts specifically on halogenated acetates to yield glycolate. The other group, termed 2-haloacid dehalogenase (EC 3.8.1 .2), acts broadly on short-chain 2-haloalkanoic acids to produce 2-hydroxy acids. However, individual enzymes in each group exhibit different substratespecificitiesfor carbon chain ...
... acts specifically on halogenated acetates to yield glycolate. The other group, termed 2-haloacid dehalogenase (EC 3.8.1 .2), acts broadly on short-chain 2-haloalkanoic acids to produce 2-hydroxy acids. However, individual enzymes in each group exhibit different substratespecificitiesfor carbon chain ...
Birth of fertile bimaternal offspring following
... S1B), consistent with a previous finding that mice with a maternally inherited IG-DMR deletion died at later gestational stages [12]. According to the Mendelian ratio, the real litter size of bimaternal mice was calculated as 5.8 ± 0.9, comparable to the litter size of WT females (6.2 ± 0.7), indica ...
... S1B), consistent with a previous finding that mice with a maternally inherited IG-DMR deletion died at later gestational stages [12]. According to the Mendelian ratio, the real litter size of bimaternal mice was calculated as 5.8 ± 0.9, comparable to the litter size of WT females (6.2 ± 0.7), indica ...
Non-Mendelian Inheritance
... maternal transmission of cpDNA is governed by a methylation-restriction system analogous to that found in bacteria: after gametic fusion, the mt– cpDNA is digested by a restriction enzyme while the modified mt+ cpDNA remains unaffected. Modification is assumed to occur as a result of methylation. St ...
... maternal transmission of cpDNA is governed by a methylation-restriction system analogous to that found in bacteria: after gametic fusion, the mt– cpDNA is digested by a restriction enzyme while the modified mt+ cpDNA remains unaffected. Modification is assumed to occur as a result of methylation. St ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).