Module 7 – Microbial Molecular Biology and Genetics
... pairing. Here, purines form hydrogen bonds to pyrimidines, with A bonding only to T, and C bonding only to G. This arrangement of two nucleotides binding together across the double helix is called a base pair. As hydrogen bonds are not covalent, they can be broken and rejoined relatively easily. The ...
... pairing. Here, purines form hydrogen bonds to pyrimidines, with A bonding only to T, and C bonding only to G. This arrangement of two nucleotides binding together across the double helix is called a base pair. As hydrogen bonds are not covalent, they can be broken and rejoined relatively easily. The ...
Clustering Method for Repeat Analysis in DNA sequences
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
PPT
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
... sequences have a BLAST E-value less than a UserSpecified Threshold when compared to any sequence in another class If a class appears in multiple similarity pairs, all these similar classes are merged with the original class ...
RNA-Seq analysis
... Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing Technology (typical read length: 30-400 bp depending on technology) RNA-Seq analysis ...
... Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing Technology (typical read length: 30-400 bp depending on technology) RNA-Seq analysis ...
Bioinformatics with basic local alignment search tool (BLAST) and
... between pairs of sequences, based on regions of local similarity (Altschul et al., 1990; Pearson and Lipman, 1988; Luscombe et al., 2001). BLAST and FASTA have therefore become fundamental tools of biology and it is essential to know how they operate, the tasks they can accomplish and how to accurat ...
... between pairs of sequences, based on regions of local similarity (Altschul et al., 1990; Pearson and Lipman, 1988; Luscombe et al., 2001). BLAST and FASTA have therefore become fundamental tools of biology and it is essential to know how they operate, the tasks they can accomplish and how to accurat ...
Protein–DNA Interactions: Amino Acid Conservation and the Effects
... where binding is specific and all members of the family target the same DNA sequence; and (iii) multi-specific, where binding is also specific, but individual family members target different DNA sequences. Overall, protein residues in contact with the DNA are better conserved than the rest of the pr ...
... where binding is specific and all members of the family target the same DNA sequence; and (iii) multi-specific, where binding is also specific, but individual family members target different DNA sequences. Overall, protein residues in contact with the DNA are better conserved than the rest of the pr ...
Molecular Cloning of Clostridium Perfringens Type B Vaccine Strain
... indicates its high sensitivity. It is unstable in high ...
... indicates its high sensitivity. It is unstable in high ...
GENtle, a free multi-purpose molecular biology tool
... Bioinformatics, also called computational biology, uses methods from informatics, statistics, computer science, and applied mathematics to work on problems of molecular biology, especially biochemistry and genetics. Once a new domain restricted to a few experts, applications of bioinformatics today ...
... Bioinformatics, also called computational biology, uses methods from informatics, statistics, computer science, and applied mathematics to work on problems of molecular biology, especially biochemistry and genetics. Once a new domain restricted to a few experts, applications of bioinformatics today ...
Detection and copy number estimation of the transgenic nucleotide
... of 215 bp was amplified with gene specific primers of cry1Ac. Primers for other transgenes such as gna and bar were also employed; however, no amplification was detected. The presence of the p35s, sps, and nptII genes was confirmed by qualitative real-time PCR. The specificity of the respective PCR ...
... of 215 bp was amplified with gene specific primers of cry1Ac. Primers for other transgenes such as gna and bar were also employed; however, no amplification was detected. The presence of the p35s, sps, and nptII genes was confirmed by qualitative real-time PCR. The specificity of the respective PCR ...
Chapter 21
... The length of the direct repeat is 5 to 9 bp and is characteristic for any particular transposon. ...
... The length of the direct repeat is 5 to 9 bp and is characteristic for any particular transposon. ...
Sequencing for all. - Thermo Fisher Scientific
... making it simple and affordable. It’s about openly sharing your methods and data so people can not only evaluate the technology, but build on it. Ion Torrent has opened its protocols, datasets, and source code to the world to enable the community to drive application development and help make sequen ...
... making it simple and affordable. It’s about openly sharing your methods and data so people can not only evaluate the technology, but build on it. Ion Torrent has opened its protocols, datasets, and source code to the world to enable the community to drive application development and help make sequen ...
Anabaena - Oxford Academic
... Geneclean kit (Bio 101 Inc). Chromosomal DNA was restricted using various restriction enzymes, according to the manufacturers’ instructions. Southern blotting of this DNA was performed using nitrocellulose (HybondC, Amersham plc) as described by Maniatis et al. [ 141. DNA fragments used for probes w ...
... Geneclean kit (Bio 101 Inc). Chromosomal DNA was restricted using various restriction enzymes, according to the manufacturers’ instructions. Southern blotting of this DNA was performed using nitrocellulose (HybondC, Amersham plc) as described by Maniatis et al. [ 141. DNA fragments used for probes w ...
A nomenclature for restriction enzymes, DNA methyltransferases
... both REase activity and MTase activity fused in a single protein to be designated RM.Eco57I. Its accompanying MTase would remain as M.Eco57I. Note that the current convention of permitting the REase to be named either with or without the `R' pre®x will be continued. Thus, R.EcoRI and EcoRI will be c ...
... both REase activity and MTase activity fused in a single protein to be designated RM.Eco57I. Its accompanying MTase would remain as M.Eco57I. Note that the current convention of permitting the REase to be named either with or without the `R' pre®x will be continued. Thus, R.EcoRI and EcoRI will be c ...
The sequence of a gene encoding convicilin from pea
... lines has allowed a convicilin locus, designated 'cvc', to be mapped to chromosome 2 in pea [7]; it is distinct from any vicilin locus so far identified [8,9]. Convicilin has been shown to be encoded by a small gene family; hybridization of the cDNA clones pCD 59 and pCD 75 (a longer version of pCD ...
... lines has allowed a convicilin locus, designated 'cvc', to be mapped to chromosome 2 in pea [7]; it is distinct from any vicilin locus so far identified [8,9]. Convicilin has been shown to be encoded by a small gene family; hybridization of the cDNA clones pCD 59 and pCD 75 (a longer version of pCD ...
DNAse I Qualification and Sample Treatment | Molecular Devices
... To validate that the assay signal and subsequent quantitation generated by the Total DNA Assay specifically measures DNA and not a non-DNA component of the sample, or To confirm that observed inhibition of the Total DNA Assay is caused by the presence of small fragment DNA, rather than protein or buff ...
... To validate that the assay signal and subsequent quantitation generated by the Total DNA Assay specifically measures DNA and not a non-DNA component of the sample, or To confirm that observed inhibition of the Total DNA Assay is caused by the presence of small fragment DNA, rather than protein or buff ...
Cutting Edge: DNA Polymerases and Are Dispensable for Ig Gene
... whose contribution is crucial to diversify the third complementarity-determining region of Ig and TCR genes during V(D)J rearrangement; 2) despite a ubiquitous expression, a higher level of transcription in lymphoid tissues, in particular B cells from tonsils, but also, and obviously not in favor of ...
... whose contribution is crucial to diversify the third complementarity-determining region of Ig and TCR genes during V(D)J rearrangement; 2) despite a ubiquitous expression, a higher level of transcription in lymphoid tissues, in particular B cells from tonsils, but also, and obviously not in favor of ...
Nucleotide
... (2) RNA Transcription—a process of converting or “transcribing” the deoxyribonucleotide sequence within one strand of DNA into single-stranded RNAs such as the messenger RNA (or mRNA) (3) RNA Translation—a process whereby the genetic information carried by mRNA is “translated” into the synthesis of ...
... (2) RNA Transcription—a process of converting or “transcribing” the deoxyribonucleotide sequence within one strand of DNA into single-stranded RNAs such as the messenger RNA (or mRNA) (3) RNA Translation—a process whereby the genetic information carried by mRNA is “translated” into the synthesis of ...
Sample pages 2 PDF
... evolve in ways that vertical transmission of random mutations from mother to daughter cells will not facilitate. Therefore, the study of these genetic exchange mechanisms will reveal a set of strategies that bacteria use to evolve and adapt, frequently in ways that allow them to interact with eukary ...
... evolve in ways that vertical transmission of random mutations from mother to daughter cells will not facilitate. Therefore, the study of these genetic exchange mechanisms will reveal a set of strategies that bacteria use to evolve and adapt, frequently in ways that allow them to interact with eukary ...
statistical issues in the analysis of microbial communities in soil
... variable sequences. This structure allows for effective discrimination between different microorganisms (Pace et al., 1986). For example, some of the more highly conserved nucleotide sequences common to all bacteria can be used to distinguish bacteria from other organisms, while some of the more var ...
... variable sequences. This structure allows for effective discrimination between different microorganisms (Pace et al., 1986). For example, some of the more highly conserved nucleotide sequences common to all bacteria can be used to distinguish bacteria from other organisms, while some of the more var ...
Gel Electrophoresis
... The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relatively thick filaments. This fact allows the preparation of ...
... The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relatively thick filaments. This fact allows the preparation of ...
Frameshift mutations of RIZ, but no point mutations in RIZ1
... instructions with the following PCR conditions: 25 cycles of 958C for 30 s, 608C for 4 min. The PCR products were sequenced with the ABI 310 DNA sequencer (Applied Biosystems). Each mutation was veri®ed by a second sequencing reaction of an independent ampli®cation product. Assessment of LOH The RIZ ...
... instructions with the following PCR conditions: 25 cycles of 958C for 30 s, 608C for 4 min. The PCR products were sequenced with the ABI 310 DNA sequencer (Applied Biosystems). Each mutation was veri®ed by a second sequencing reaction of an independent ampli®cation product. Assessment of LOH The RIZ ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).