• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Pierre MADL Div. of Material Sciences Dep
Pierre MADL Div. of Material Sciences Dep

... stem cells become the various pluripotent cell lines of the embryo which in turn become fully differentiated cells. In other words, a single fertilized egg cell - the zygote - changes into the many cell types including neurons, muscle cells, epithelium, blood vessels et cetera as it continues to div ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... Prokaryotic and eukaryotic cells can, through mitosis, serve as factories to make many copies (> 106 in some cases) of a moderately complex segment of DNA—provided that that segment can be incorporated into a chromosome or a plasmid This is amplification ...
Lack of expression of XIST from a small ring X chromosome
Lack of expression of XIST from a small ring X chromosome

... observed (Figure 3C), although one allele was fainter in both DNA and cDNA, presumably due to mosaicism for the ring chromosome. Molecular characterisation of the ring X chromosome DNA isolated from the mother, the father and the patient was amplified with primers for polymorphic X-linked markers. I ...
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)

... AmpC β-lactamases are cephalosporinases encoded on the chromosomes of many of the Enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, and β-lactamase inhibitor-β-lactam combinations. [1]. The emission of bacteria into th ...
BLOTTING.142
BLOTTING.142

...  The large fragments move more slowly than ...
Translational selection is operative for synonymous codon usage in
Translational selection is operative for synonymous codon usage in

... P<0?0001). These results support our interpretation that the first axis discriminates expression levels. The second axis of the COA (6?7 % of the variability) discriminates between genes located in the leading or lagging strand of replication. The importance of this effect can be so high that in spe ...
PDF Print - APS Journals
PDF Print - APS Journals

... mutualistic. Both phenotypes, however, are represented by organisms with different mechanisms of interaction with the plant host. Biotrophic mechanisms, for example, can be found in pathogens and mutualists. Moreover, some symbionts are not characterized by a single mechanism; they change the type o ...
Molecular Diagnostics in Clinical Microbiology
Molecular Diagnostics in Clinical Microbiology

... as a consequence of an antimicrobial therapy in combination with the host immune response, can be measured with quantitative diagnostic NA approaches. A number of currently developed molecular-based techniques, such as whole genome sequencing, may play an important role in the development of new scr ...
Evolutionary origin and consequences of uniparental mitochondrial
Evolutionary origin and consequences of uniparental mitochondrial

... has been investigated, the male sterile phenotype is caused by a mitochondrial mutation; elimination of the male function does not affect the transmission prospects of mitochondrial genes (as long as there is no overall shortage of pollen in the population). Indeed, a mitochondrial mutation that eli ...
slides
slides

... Can also cause degeneration of muscles or nerves ...
Expression of the six chromate ion transporter
Expression of the six chromate ion transporter

... homologues share 26–50 % amino acid sequence identity and 28–55 % sequence similarity among them (percentages for each homologous pair are presented in Table S2). Analysis of the genomic context of B. xenovorans chr genes showed that the chrA2 homologue, encoded on the megaplasmid replicon, forms pa ...
Allele Mining Strategies: Principles and Utilisation for Blast
Allele Mining Strategies: Principles and Utilisation for Blast

... through gene pyramiding. The actual identification of candidate DNA markers linked to resistance genes using fine mapping may well allow rice breeders to efficiently transfer these genes from donor cultivars into new, elite rice cultivars using marker-assisted selection. There is also a need to conn ...
The hybrid origins of three perennial Medicago species
The hybrid origins of three perennial Medicago species

... lysates that some plant materials can generate. Lysates affect the DNA by shearing and would result into smaller fragments. After a few steps with centrifuging and removing of the precipitates, adding buffer AP3/E (for 30 min into the clear lysate) binds to the DNA and result in a higher yield. The ...
Genomic rearrangements account for more than one
Genomic rearrangements account for more than one

... in the Dutch patients (2). However, in most cases, these studies were probably limited by the complexity and sensitivity of the technical approaches employed. For instance, in order to achieve a good accuracy, the commonly used Southern blot analysis should be carried out using several well-designed ...
Pairing of homologous regions in the mouse genome is associated
Pairing of homologous regions in the mouse genome is associated

... proximity, we used whole chromosome painting together with probes marking the KvDMR region and a region near the centromeric end of chromosome 7. Most nuclei showed two separate chromosome 7 domains which tended to be positioned away from each other (Fig. 3A, Movie S1). When KvDMR signals were paire ...
Chromosomal breakpoint positions suggest a direct role for radiation
Chromosomal breakpoint positions suggest a direct role for radiation

What are chromosomes?
What are chromosomes?

... What are chromosomes? Chromosomes are the structures that hold our genes  Genes are the individual instructions that tell our bodies how to develop and function They govern our physical and medical characteristics, such as hair color, blood type and susceptability to disease.  Each chromosome ha ...
RHD - Labex
RHD - Labex

... Amplification of the maize DNA added to each plasma and control provides a control for: ...
Lactobacilli carry cryptic genes encoding peptidase
Lactobacilli carry cryptic genes encoding peptidase

Linkage Mapping 2 3 – point linkage mapping One crossover Two
Linkage Mapping 2 3 – point linkage mapping One crossover Two

... • Any number of crossovers greater than zero will produce 50% recombinant gametes on average. • This is why recombination frequency is not a linear function of the average number of crossovers between two loci. • If loci are widely separated on the chromosome, several crossovers may occur between th ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... nodM, that is specialized for symbiosis in Rhizobium leguminosarum (12); there are two copies of nodPQ (30) and two fixN regions in Rhizobium meliloti (24) and two nifHDK operons in Rhizobium etli (22). The additional copies of these genes may be the result of gene duplication. In the case of the R. ...
Trawling DNA Databases For Partial Matches: What Is The FBI
Trawling DNA Databases For Partial Matches: What Is The FBI

... DNA evidence is often presented as the “gold standard” for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers, dozens of judicial opinions, and two comm ...
TEL Gene Is Involved in Myelodysplastic Syndromes
TEL Gene Is Involved in Myelodysplastic Syndromes

... shown). Direct sequencing showed that cTEL5‘ contained a TEL exon which ends at bp 187 of the TEL cDNA sequence, and no sequence derived downstream from this exon. Thus, cosmid TEL5‘ contains no sequences derived downstream from the breakpoint in the t(5;12) case published by Golub et al.I3 On the o ...
Alu
Alu

Localization and structural analysis of the ribosomal RNA operons of
Localization and structural analysis of the ribosomal RNA operons of

< 1 ... 22 23 24 25 26 27 28 29 30 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report