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... alignment gaps). The dataset included 85 first introns of median length 159 bp (mean length 357 bp), whereas non-first introns had median length 118 bp (mean 210 bp). It should be noted that only first intron pairs in which both introns were first introns were considered, and the same criterion was ...
... alignment gaps). The dataset included 85 first introns of median length 159 bp (mean length 357 bp), whereas non-first introns had median length 118 bp (mean 210 bp). It should be noted that only first intron pairs in which both introns were first introns were considered, and the same criterion was ...
Plant and Soil.
... related and showed that Rh&obium and Bradyrhizobium are related to other groups of bacteria that are not plant symbionts. Rhizobium and Agrobacterium species are intermixed. Differences in plasmid content may explain to a good extent the different behavior of Rhizobium and Agrobacterium as symbionts ...
... related and showed that Rh&obium and Bradyrhizobium are related to other groups of bacteria that are not plant symbionts. Rhizobium and Agrobacterium species are intermixed. Differences in plasmid content may explain to a good extent the different behavior of Rhizobium and Agrobacterium as symbionts ...
induction of instability at selected loci in maize
... 1:3 ratio will occur if the plants are not homozygous for the mutation (i.e. there is only one a1m‐4). 1/2 of gametes wil posses Ac, while only 1/4 of the gametes will possess the mutation. Therefore, less kernels will the mutation in which Ds can "jump out" of, producing less variegated kernels. 2 ...
... 1:3 ratio will occur if the plants are not homozygous for the mutation (i.e. there is only one a1m‐4). 1/2 of gametes wil posses Ac, while only 1/4 of the gametes will possess the mutation. Therefore, less kernels will the mutation in which Ds can "jump out" of, producing less variegated kernels. 2 ...
Supporting Information Parfenov et al. 10.1073/pnas.1416074111
... they also did not show statistically significant association. Additionally, Fisher’s exact test was used to test for associations between virus integration and other clinical factors as well as E6/E7 expression (Fig. S1C). FISH. DNA from two fosmids, G248P80924G11 and G248P86073A9, ...
... they also did not show statistically significant association. Additionally, Fisher’s exact test was used to test for associations between virus integration and other clinical factors as well as E6/E7 expression (Fig. S1C). FISH. DNA from two fosmids, G248P80924G11 and G248P86073A9, ...
Trachemys scripta elegans Red-Eared Turtle ( Fc)
... The 115 unique VH clones accounted for a total of 61 unique CDR3s, which resulted from independent VDJ recombination events. The lengths of the CDR3s (according to the IMGT numbering system) among these clones ranged from 7 to 21 aa (Supplemental Fig. 2), with an average length of 11.4 6 2.5 aa. Alt ...
... The 115 unique VH clones accounted for a total of 61 unique CDR3s, which resulted from independent VDJ recombination events. The lengths of the CDR3s (according to the IMGT numbering system) among these clones ranged from 7 to 21 aa (Supplemental Fig. 2), with an average length of 11.4 6 2.5 aa. Alt ...
DNA breathing dynamics distinguish binding from nonbinding
... fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG ...
... fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG ...
Direct and indirect consequences of meiotic recombination
... the direct effects of recombination (further details are presented in Box 1 and Figure 1). Although one of the advantages of the indirect effects of recombination is the efficient removal of deleterious alleles from a population, its direct effects may sometimes themselves be harmful. In particular, ...
... the direct effects of recombination (further details are presented in Box 1 and Figure 1). Although one of the advantages of the indirect effects of recombination is the efficient removal of deleterious alleles from a population, its direct effects may sometimes themselves be harmful. In particular, ...
New peptide and gene coding for same
... (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisting of synthesized 14 meroligonucleotides labeled with 32P coding for an amino acid sequence of from 12 to 16 of a-hANP (Fig. 9), and 23 clones were selected which hybridized with the probes. Among the 23 c ...
... (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisting of synthesized 14 meroligonucleotides labeled with 32P coding for an amino acid sequence of from 12 to 16 of a-hANP (Fig. 9), and 23 clones were selected which hybridized with the probes. Among the 23 c ...
Dynamic changes of yak (Bos grunniens) gut microbiota during
... PCA was performed (on mean-centered data) to visualize the general structure of species-level composition of ...
... PCA was performed (on mean-centered data) to visualize the general structure of species-level composition of ...
Chromosomes in Saccharomyces cerevisiae
... Integration of YLp16 into the circular version of chromosome III relinearizes the chromosome and makes the LEU2 and URA3 markers, originally carried by the plasmid, mitotically stable. Therefore, continuous growth in leucine-free medium enriches for integrants. We determined that the integrant TA114 ...
... Integration of YLp16 into the circular version of chromosome III relinearizes the chromosome and makes the LEU2 and URA3 markers, originally carried by the plasmid, mitotically stable. Therefore, continuous growth in leucine-free medium enriches for integrants. We determined that the integrant TA114 ...
transposon
... present in reverse orientation at the ends of some transposons. direct repeats : identical (or closely related) sequences present in two or more copies in the same orientation; they are not necessarily adjacent. transposase : the enzyme involved in insertion of transposon at a new site. ...
... present in reverse orientation at the ends of some transposons. direct repeats : identical (or closely related) sequences present in two or more copies in the same orientation; they are not necessarily adjacent. transposase : the enzyme involved in insertion of transposon at a new site. ...
The promiscuous primase
... DNA primases are essential for the initiation of DNA replication and progression of the replication fork. Recent phylogenetic analyses coupled with biochemical and structural studies have revealed that the arrangement of catalytic residues within the archaeal and eukaryotic primase has significant s ...
... DNA primases are essential for the initiation of DNA replication and progression of the replication fork. Recent phylogenetic analyses coupled with biochemical and structural studies have revealed that the arrangement of catalytic residues within the archaeal and eukaryotic primase has significant s ...
The Large Loop Repair and Mismatch Repair Pathways
... in aberrant segregation frequency in the ⌬rad1 strains occurred in strains expected to form loops of 16 bases or greater. In contrast, the ⌬pms1 derivatives exhibited elevated aberrant segregation frequencies in strains expected to form small loops, but not large loops. Recombination was significant ...
... in aberrant segregation frequency in the ⌬rad1 strains occurred in strains expected to form loops of 16 bases or greater. In contrast, the ⌬pms1 derivatives exhibited elevated aberrant segregation frequencies in strains expected to form small loops, but not large loops. Recombination was significant ...
View/Open
... to larger phenotypic effect on a trait. In contrast, trans-eQTLs are not physically located near the gene and hence trans-eQTL causes variation in the expression of genes due to polymorphism in a regulatory factor. These regulatory factors can be located elsewhere in the genome and many regulatory f ...
... to larger phenotypic effect on a trait. In contrast, trans-eQTLs are not physically located near the gene and hence trans-eQTL causes variation in the expression of genes due to polymorphism in a regulatory factor. These regulatory factors can be located elsewhere in the genome and many regulatory f ...
Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama
... adhesion molecules (Ishii et al., 2014a), and also by inhibiting wound healing, which leads to a massive loss of hemolymph in silkworm larvae (Ishii et al., 2014b). Cecropins are used by insect as the main defence against gram-negative bacteria. Three different proteases exist in S. marcescens can d ...
... adhesion molecules (Ishii et al., 2014a), and also by inhibiting wound healing, which leads to a massive loss of hemolymph in silkworm larvae (Ishii et al., 2014b). Cecropins are used by insect as the main defence against gram-negative bacteria. Three different proteases exist in S. marcescens can d ...
Journal of Bacteriology
... supplemented with tetracycline. The cosmids of these seven colonies were isolated, digested with EcoRI, and again analyzed in a Southern blot assay using the same ropA probe as was used in the colony blot assay. Of these seven cosmid clones, two contained the 2.1-kb EcoRI fragment on which ropA is l ...
... supplemented with tetracycline. The cosmids of these seven colonies were isolated, digested with EcoRI, and again analyzed in a Southern blot assay using the same ropA probe as was used in the colony blot assay. Of these seven cosmid clones, two contained the 2.1-kb EcoRI fragment on which ropA is l ...
Amiito acid sequence of the testosterone
... used in primer-extension analysis and the 1060 bp Bgl I fragment used in the screen of the genomlc library are also indicated. The sequencing strategy employed for each clone is shown below the maps. Closed circles indicate fragments sequenced from their 5' ends and open dries Indicate those sequenc ...
... used in primer-extension analysis and the 1060 bp Bgl I fragment used in the screen of the genomlc library are also indicated. The sequencing strategy employed for each clone is shown below the maps. Closed circles indicate fragments sequenced from their 5' ends and open dries Indicate those sequenc ...
Major City Chiefs Position Paper on Sworn vs
... course of a criminal investigations or criminal court proceedings; and in which the analysis and interpretation should be properly conducted by a qualified scientific or technical expert, utilizing quality standards and processes. Any current or future discipline that is not expressly named in this ...
... course of a criminal investigations or criminal court proceedings; and in which the analysis and interpretation should be properly conducted by a qualified scientific or technical expert, utilizing quality standards and processes. Any current or future discipline that is not expressly named in this ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.