Data visualization in the post
... – ‘Plasticity’ of the genome – Spatial and temporal regulation ...
... – ‘Plasticity’ of the genome – Spatial and temporal regulation ...
chapter 19_updates
... DNA at specific nucleotide sequences • Type II restriction enzyme: most useful enzyme • By adding methyl groups to the recognition sequence to protect itself from being digested by its own enzyme in bacteria ...
... DNA at specific nucleotide sequences • Type II restriction enzyme: most useful enzyme • By adding methyl groups to the recognition sequence to protect itself from being digested by its own enzyme in bacteria ...
The Genome of Theobroma Cacao
... long strings of these four letters (one string for each chromosome) for a total of 430 million letters. The recent development of new technologies has made DNA sequencing dramatically easier and cheaper, and the number of complete genome sequences is growing rapidly. The study of the global properti ...
... long strings of these four letters (one string for each chromosome) for a total of 430 million letters. The recent development of new technologies has made DNA sequencing dramatically easier and cheaper, and the number of complete genome sequences is growing rapidly. The study of the global properti ...
SW describe how techniques such as DNA
... The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual ...
... The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual ...
Restriction Enzymes, Vectors, and Genetic Libraries
... . Link these fragments to selfreplicating forms of DNA = vectors. ...
... . Link these fragments to selfreplicating forms of DNA = vectors. ...
Slide 1
... Archibald Garrod, observes that the disease alkaptonuria has a genetic cause and is inherited as a recessive condition. ...
... Archibald Garrod, observes that the disease alkaptonuria has a genetic cause and is inherited as a recessive condition. ...
People Pieces
... Living organisms are made of cells and every cell is designed to perform a specific function in the organism. Humans are made of trillions of cells, and the design and function of each cell, and thus of the organism, is controlled by genes. Each human cell contains 46 chromosomes, which are strands ...
... Living organisms are made of cells and every cell is designed to perform a specific function in the organism. Humans are made of trillions of cells, and the design and function of each cell, and thus of the organism, is controlled by genes. Each human cell contains 46 chromosomes, which are strands ...
Recombinant DNA technology.ppt [Compatibility Mode]
... Restriction endonucleases • Also called restriction enzymes • Occur naturally in bacteria • Hundreds are purified and available commercially • Named for bacterial genus, species, strain, and type ...
... Restriction endonucleases • Also called restriction enzymes • Occur naturally in bacteria • Hundreds are purified and available commercially • Named for bacterial genus, species, strain, and type ...
Faber: Sequence resources
... available, recovered BACs/YACs during HGP PCR much cheaper than BAC/YAC sequencing Represent the superposition (i.e. can also be double-pass reads) Fingerprint clone contigs bound to specific STSs ...
... available, recovered BACs/YACs during HGP PCR much cheaper than BAC/YAC sequencing Represent the superposition (i.e. can also be double-pass reads) Fingerprint clone contigs bound to specific STSs ...
The Human Genome Project CH 13 Sec 3 notes
... The Human Genome Project CH 13 Sec 3 Human Genome Project (HGB) •International project •Began in 1990, completed in ________ •Objective: ________________________________________________________ –DNA sequencing •Many other genomes have now been sequenced Sequencing the genome •The 46 chromosomes were ...
... The Human Genome Project CH 13 Sec 3 Human Genome Project (HGB) •International project •Began in 1990, completed in ________ •Objective: ________________________________________________________ –DNA sequencing •Many other genomes have now been sequenced Sequencing the genome •The 46 chromosomes were ...
Lecture
... Fosmid/Cosmid----Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged in lambda phage particles for infection into E. coli; this permits cloning of larger DNA fragments (up to 45kb) than can be introduced into bacterial hosts in plasmid vectors. ...
... Fosmid/Cosmid----Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged in lambda phage particles for infection into E. coli; this permits cloning of larger DNA fragments (up to 45kb) than can be introduced into bacterial hosts in plasmid vectors. ...
Genes and genomes
... experiment – it contains tightly coiled DNA around special proteins. The DNA of a human being’s 23 chromosomes, when uncoiled, is 2 meters long!! ...
... experiment – it contains tightly coiled DNA around special proteins. The DNA of a human being’s 23 chromosomes, when uncoiled, is 2 meters long!! ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.