Katie-Arabidopsis
... • No immediate agricultural importance and is not thought to cure any disease • Prolific seed production and easy cultivation in restricted space • A large number of mutant lines and genomic resources ...
... • No immediate agricultural importance and is not thought to cure any disease • Prolific seed production and easy cultivation in restricted space • A large number of mutant lines and genomic resources ...
BIOL 433 Plant Genetics Term 1, 2005
... Isolated genomic DNA contains many copies of each chromosome (from many cells) ...
... Isolated genomic DNA contains many copies of each chromosome (from many cells) ...
BIOL 433 Plant Genetics Term 1, 2005
... Isolated genomic DNA contains many copies of each chromosome (from many cells) ...
... Isolated genomic DNA contains many copies of each chromosome (from many cells) ...
Human Genome Project
... • First produced a clone-based physical map of the genome that would serve as a scaffold for the later sequence data: – Broke genome into chunks of DNA whose position on chromosome was known from maps, clone into bacteria using BACs. – Digest BAC-inserted clonal chunks of DNA into small fragments. – ...
... • First produced a clone-based physical map of the genome that would serve as a scaffold for the later sequence data: – Broke genome into chunks of DNA whose position on chromosome was known from maps, clone into bacteria using BACs. – Digest BAC-inserted clonal chunks of DNA into small fragments. – ...
The Future of Genetics Research - Blyth-Biology11
... information • Today we have huge databases of protein and DNA information • NCBI ...
... information • Today we have huge databases of protein and DNA information • NCBI ...
Epigenetics
... indirectly affects the DNA in your genome. • Histones are proteins which enable DNA's molecules to be wound up neatly into chromosomes inside the cell nucleus. • A variety of chemical tags can grab hold of the tails of histones, changing how tightly or loosely they package DNA. • If the wrapping is ...
... indirectly affects the DNA in your genome. • Histones are proteins which enable DNA's molecules to be wound up neatly into chromosomes inside the cell nucleus. • A variety of chemical tags can grab hold of the tails of histones, changing how tightly or loosely they package DNA. • If the wrapping is ...
Sequencing a genome
... finished). Whole Genome Shotguns are referred to as having an X-fold coverage. Low coverage (2x) is sufficient for gene discovery and some regulatory element identification. High coverage (6x) is good for gene annotation. There will still be some missing genes. Finished sequence has no gaps and is p ...
... finished). Whole Genome Shotguns are referred to as having an X-fold coverage. Low coverage (2x) is sufficient for gene discovery and some regulatory element identification. High coverage (6x) is good for gene annotation. There will still be some missing genes. Finished sequence has no gaps and is p ...
AP Biology - gwbiology
... 14. How are bacteriophages used for making genomic libraries and what are some of the advantages of this? ...
... 14. How are bacteriophages used for making genomic libraries and what are some of the advantages of this? ...
AP Biology
... 30. Outline the diagram below of Dideoxy Chain Termination – I know this seems difficult to follow at first but at least copy the main ideas before we go over it in class. ...
... 30. Outline the diagram below of Dideoxy Chain Termination – I know this seems difficult to follow at first but at least copy the main ideas before we go over it in class. ...
7echap20guidedreading
... 2. What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the “cloned gene of interest.” ...
... 2. What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the “cloned gene of interest.” ...
Chapter 2 PowerPoint Slides
... that amplifies a distinct portion of the genome • chromosomes were fragmented and inserted into bacteria and/or yeast -- to maintain the DNA • bacterial vectors carried approximately 150 kb of sequence -- BAC (E. coli.) • YACs -- 150 kb to 1.5 Mb • Using restriction maps, and the STSs, the BACs and ...
... that amplifies a distinct portion of the genome • chromosomes were fragmented and inserted into bacteria and/or yeast -- to maintain the DNA • bacterial vectors carried approximately 150 kb of sequence -- BAC (E. coli.) • YACs -- 150 kb to 1.5 Mb • Using restriction maps, and the STSs, the BACs and ...
Human Genome Project, Gene Therapy, and Cloning
... To sequence & determine the exact order of the nucleotides (A,C,T,G) for ALL of the DNA in a human cell To determine which sections of DNA represent the individual genes To store this information in databases for analysis ...
... To sequence & determine the exact order of the nucleotides (A,C,T,G) for ALL of the DNA in a human cell To determine which sections of DNA represent the individual genes To store this information in databases for analysis ...
PowerPoint Presentation - No Slide Title
... In eukaryotes, DNA is present as several different molecules. Each DNA molecule, along with its associated proteins is one chromosome. Chromosomes are in the extended conformation while they are being transcribed. They are at their most condensed during nuclear division. ...
... In eukaryotes, DNA is present as several different molecules. Each DNA molecule, along with its associated proteins is one chromosome. Chromosomes are in the extended conformation while they are being transcribed. They are at their most condensed during nuclear division. ...
MS Word
... Sequencing was done at least three times either on both strands or using both dye primer and terminator chemistries. The assembly of the contigs and their integrity were verified after in silico construction of the “minimum tiling path.” Relevant recombinant BACs were analyzed by four restriction en ...
... Sequencing was done at least three times either on both strands or using both dye primer and terminator chemistries. The assembly of the contigs and their integrity were verified after in silico construction of the “minimum tiling path.” Relevant recombinant BACs were analyzed by four restriction en ...
AP-ppt-PCR
... Not all bacteria pick up plasmid-how do we distinguish? Annealing of human DNA to plasmid is random-how do we distinguish which plasmids have human DNA? ...
... Not all bacteria pick up plasmid-how do we distinguish? Annealing of human DNA to plasmid is random-how do we distinguish which plasmids have human DNA? ...
E co
... (a)The use of linkers to create tailor-made ends on cloning fragments.Synthetic oligonucleotide duplexes whose sequences represent EcoRI restriction sites are blunt-end ligated to a DNA molecule using T4DNA ligase.Note that the ligation reaction can add multiple linkers on each end of the blunt-end ...
... (a)The use of linkers to create tailor-made ends on cloning fragments.Synthetic oligonucleotide duplexes whose sequences represent EcoRI restriction sites are blunt-end ligated to a DNA molecule using T4DNA ligase.Note that the ligation reaction can add multiple linkers on each end of the blunt-end ...
Lec15-Recombinant
... Insert DNA into vectors that can replicate in bacteria Transform (introduce) DNA into host cell Plate cells and select those with vectors Each colony has one chunk of DNA The whole set is a library of human DNA ...
... Insert DNA into vectors that can replicate in bacteria Transform (introduce) DNA into host cell Plate cells and select those with vectors Each colony has one chunk of DNA The whole set is a library of human DNA ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.