Comparative genomic analysis of carbon and nitrogen assimilation
... Background: Carbon and nitrogen fixation are essential pathways for autotrophic bacteria living in extreme environments. These bacteria can use carbon dioxide directly from the air as their sole carbon source and can use different sources of nitrogen such as ammonia, nitrate, nitrite, or even nitrog ...
... Background: Carbon and nitrogen fixation are essential pathways for autotrophic bacteria living in extreme environments. These bacteria can use carbon dioxide directly from the air as their sole carbon source and can use different sources of nitrogen such as ammonia, nitrate, nitrite, or even nitrog ...
Microbiology
... sp. GRHZ were isolated and found to harbour different alterations in surface polysaccharides. These mutants, designated GRHZ-14 and GRHZ-50, induced a few, empty nodules on Acacia and lost the ability to nodulate most host herbaceous legumes. Whereas mutant GRHZ-14 produces an acidic exopolysacchari ...
... sp. GRHZ were isolated and found to harbour different alterations in surface polysaccharides. These mutants, designated GRHZ-14 and GRHZ-50, induced a few, empty nodules on Acacia and lost the ability to nodulate most host herbaceous legumes. Whereas mutant GRHZ-14 produces an acidic exopolysacchari ...
The Biology and Evolution of Mammalian Y Chromosomes
... fosmids are used as sequencing templates because they are less prone to chimerism than the larger YAC clones. Second, clones derived from the Y chromosome of one individual, bearing a single haplotype, are used for sequencing to eliminate polymorphisms, which could otherwise be confused with paralog ...
... fosmids are used as sequencing templates because they are less prone to chimerism than the larger YAC clones. Second, clones derived from the Y chromosome of one individual, bearing a single haplotype, are used for sequencing to eliminate polymorphisms, which could otherwise be confused with paralog ...
Linkage analysis
... 1% chance to show a similar phenotype despite a normal genotype 90% chance to show the phenotype when 1 mutant allele (dominant with incomplete penetrance) 99% likelihood to present with the phenotype if both alleles are mutant ...
... 1% chance to show a similar phenotype despite a normal genotype 90% chance to show the phenotype when 1 mutant allele (dominant with incomplete penetrance) 99% likelihood to present with the phenotype if both alleles are mutant ...
A Mutation Causing Reduced Biological Activity and Stability of
... manufacturer (Bethesda Research Laboratories, Gaithersburg, MD). Fragments of 10-20 kbp, which are enriched for the TBG gene, were isolated by electrophoresis in a low melting ajarose gel (30) and ligated into EcoRI arms of XEMBL4. After packaging and infection of Escherichia coli strain LE 392, clo ...
... manufacturer (Bethesda Research Laboratories, Gaithersburg, MD). Fragments of 10-20 kbp, which are enriched for the TBG gene, were isolated by electrophoresis in a low melting ajarose gel (30) and ligated into EcoRI arms of XEMBL4. After packaging and infection of Escherichia coli strain LE 392, clo ...
Genetic Inversion: Relationships Among Species
... f. How do you think decisions were made regarding where to place the species? g. What other ways could they have compared these species to place them on the tree? What way do you think would give the most accurate diagram? ...
... f. How do you think decisions were made regarding where to place the species? g. What other ways could they have compared these species to place them on the tree? What way do you think would give the most accurate diagram? ...
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY
... Disclaimer: These solutions are provided by the academic team of BASE. These solutions may be different from those to be provided by the Karnataka Examinations Authority (KEA) due to difference in assumptions taken in support of the solutions. In such cases solution provided by KEA will be deemed as ...
... Disclaimer: These solutions are provided by the academic team of BASE. These solutions may be different from those to be provided by the Karnataka Examinations Authority (KEA) due to difference in assumptions taken in support of the solutions. In such cases solution provided by KEA will be deemed as ...
Chapter 10: DIPLOIDY
... diploid representations and dominance operators in genetic algorithms applied to nonstationary search problems. Analytical and experimental evidence showed that a diploid GA maintained extra diversity at loci where alternative alleles were emphasized in the recent past [Smith et al. 1992]. In effect ...
... diploid representations and dominance operators in genetic algorithms applied to nonstationary search problems. Analytical and experimental evidence showed that a diploid GA maintained extra diversity at loci where alternative alleles were emphasized in the recent past [Smith et al. 1992]. In effect ...
A Comparative Study on the Recovery of EcoRI Endonuclease from
... Restriction enzymes have extensive applications in recombinant DNA technology. They are used in the preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known r ...
... Restriction enzymes have extensive applications in recombinant DNA technology. They are used in the preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known r ...
Turnover-based in vitro selection and evolution of biocatalysts from
... were determined at 30 °C in 50 mM CHES (2-(N-cyclohexylamino)ethanesulfonic acid), 25 mM NaCl, pH 9.0. For E. coli alkaline phosphatase (AP), various kcat/kuncat values (1010–1015) were reported27,28,43,44 at 25 °C in different buffer systems, with different substrates showing great variation in kun ...
... were determined at 30 °C in 50 mM CHES (2-(N-cyclohexylamino)ethanesulfonic acid), 25 mM NaCl, pH 9.0. For E. coli alkaline phosphatase (AP), various kcat/kuncat values (1010–1015) were reported27,28,43,44 at 25 °C in different buffer systems, with different substrates showing great variation in kun ...
Sequence requirements for function of the
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
Molecular cloning of a rhodopsin gene from salamander rods.
... FIGURE 3. Absorbance spectrum, reverse transcription-polymerase chain reaction (RTPCR), and Southern blot analyses of single rod cells. (A) Isolation of single rods. A single rod cell was isolated using a micropipette, and the absorbance spectrum of the exposed outer segment was measured with a prob ...
... FIGURE 3. Absorbance spectrum, reverse transcription-polymerase chain reaction (RTPCR), and Southern blot analyses of single rod cells. (A) Isolation of single rods. A single rod cell was isolated using a micropipette, and the absorbance spectrum of the exposed outer segment was measured with a prob ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
Analysing complex genetic traits with chromosome
... We consider features of CSSs that are useful for dissecting complex traits and offer advantages over other methods. Partitioning of the genome. CSSs offer advantages over RISs and RCSs in that the contribution of the donor genome is partitioned into individual chromosomes. As a result, the pattern o ...
... We consider features of CSSs that are useful for dissecting complex traits and offer advantages over other methods. Partitioning of the genome. CSSs offer advantages over RISs and RCSs in that the contribution of the donor genome is partitioned into individual chromosomes. As a result, the pattern o ...
2q13 microduplications
... With any duplication, the amount of duplicated DNA can vary. If the amount is small it may not be possible to see it under the microscope and many people who have a microduplication may have previously been told their standard chromosome analysis was ‘normal’. A laboratory technique called FISH (flu ...
... With any duplication, the amount of duplicated DNA can vary. If the amount is small it may not be possible to see it under the microscope and many people who have a microduplication may have previously been told their standard chromosome analysis was ‘normal’. A laboratory technique called FISH (flu ...
A selfish origin for recombination
... of recombination at the molecular level. Classical population genetics models have emphasized the role of modifier genes (for a review see Feldman et al., 1996) on the evolution of recombination, that is they have considered the evolution of recombination at a certain site as something imposed by ano ...
... of recombination at the molecular level. Classical population genetics models have emphasized the role of modifier genes (for a review see Feldman et al., 1996) on the evolution of recombination, that is they have considered the evolution of recombination at a certain site as something imposed by ano ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... responsible for the disease either by culture or by real time polymerase chain reaction (RT-PCR). Indirect diagnosis is essentially by serology and consists of detecting specific antibodies in the serum of an infected individual. Culture is not very sensitive since the percentage of success is gener ...
... responsible for the disease either by culture or by real time polymerase chain reaction (RT-PCR). Indirect diagnosis is essentially by serology and consists of detecting specific antibodies in the serum of an infected individual. Culture is not very sensitive since the percentage of success is gener ...
Mapping of the Recessive White Locus and
... typed for 2 new markers (169333023 and 180893258). Figure 1 shows the genotypes of the marker used to determine gene order around the C locus. Based on the recombination between the markers, the most likely gene order and genetic distance were determined as follows: 163386361-(4.0 cM)-ABR631-(7.7 cM ...
... typed for 2 new markers (169333023 and 180893258). Figure 1 shows the genotypes of the marker used to determine gene order around the C locus. Based on the recombination between the markers, the most likely gene order and genetic distance were determined as follows: 163386361-(4.0 cM)-ABR631-(7.7 cM ...
Polycomb Group silencers collaborate with Notch pathway to cause
... the hsp70-Gal4 genotype crossed with eyeful in the absence of heat shock (—) and after 1h heat-shock (+). PCR amplification of RT lola or psq products was performed from the common region of the lola transcripts (b) or the psq BTB region (lower band in c) and the psq common region (upper bands in c) ...
... the hsp70-Gal4 genotype crossed with eyeful in the absence of heat shock (—) and after 1h heat-shock (+). PCR amplification of RT lola or psq products was performed from the common region of the lola transcripts (b) or the psq BTB region (lower band in c) and the psq common region (upper bands in c) ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.