CHAPTER 21 Chromosomal Mutations
... d. Molecular analysis shows a repeated 3-bp sequence, CGG, in the FMR-1 (fragile X mental retardation-1) gene, at the fragile X site. i. Normal individuals have 6–54 CGG repeats, with an average of 29. ii. Normal transmitting carrier males, their daughters and some other carrier females have 55–200 ...
... d. Molecular analysis shows a repeated 3-bp sequence, CGG, in the FMR-1 (fragile X mental retardation-1) gene, at the fragile X site. i. Normal individuals have 6–54 CGG repeats, with an average of 29. ii. Normal transmitting carrier males, their daughters and some other carrier females have 55–200 ...
SpliceCenter_DataBuild
... The work of Ari Kahn, Barry Zeeberg, and Hongfang Liu laid the foundation for the current splice variant database build process. Dr. Kahn and Zeeberg developed a splice variant database, EVDB [5], which was based on NCBI Evidence Viewer [8] data. EVDB demonstrated the merits of constructing a reposi ...
... The work of Ari Kahn, Barry Zeeberg, and Hongfang Liu laid the foundation for the current splice variant database build process. Dr. Kahn and Zeeberg developed a splice variant database, EVDB [5], which was based on NCBI Evidence Viewer [8] data. EVDB demonstrated the merits of constructing a reposi ...
Package `biomartr`
... Description Perform metagenomic data retrieval and functional annotation retrieval. In detail, this package aims to provide users with a standardized way to automate genome, proteome, coding sequence ('CDS'), 'GFF', and metagenome retrieval from 'NCBI' and 'ENSEMBL' databases. Furthermore, an interf ...
... Description Perform metagenomic data retrieval and functional annotation retrieval. In detail, this package aims to provide users with a standardized way to automate genome, proteome, coding sequence ('CDS'), 'GFF', and metagenome retrieval from 'NCBI' and 'ENSEMBL' databases. Furthermore, an interf ...
Comparative genomics provides evidence for the 3
... frames that show a high degree of sequence similarity to genes encoding enzymes in the C. aurantiacus pathway. Metagenomic DNA sequences from the microbial mats of alkaline siliceous hot springs also contain homologues of these genes that are highly similar to genes in both Roseiflexus spp. and Chlo ...
... frames that show a high degree of sequence similarity to genes encoding enzymes in the C. aurantiacus pathway. Metagenomic DNA sequences from the microbial mats of alkaline siliceous hot springs also contain homologues of these genes that are highly similar to genes in both Roseiflexus spp. and Chlo ...
Relatedness in the post-genomic era: is it still
... lineage paths in pedigrees (BOX 1) provides a mathematically beautiful structure that has historically been useful, but its weaknesses are apparent. Pedigree founders are typically assumed to be unrelated, but this is only realistic in certain settings, such as some designed breeding programmes or a ...
... lineage paths in pedigrees (BOX 1) provides a mathematically beautiful structure that has historically been useful, but its weaknesses are apparent. Pedigree founders are typically assumed to be unrelated, but this is only realistic in certain settings, such as some designed breeding programmes or a ...
Chromosomes Carrying Meiotic Avoidance Loci
... apomixis in Hieracium praealtum. Genetic markers linked to these loci have been determined using a deletion mapping approach (Catanach et al., 2006). The roles of these two loci and their interactions with the sexual pathway have been determined from the analyses of apomixis mutants, transgenic abla ...
... apomixis in Hieracium praealtum. Genetic markers linked to these loci have been determined using a deletion mapping approach (Catanach et al., 2006). The roles of these two loci and their interactions with the sexual pathway have been determined from the analyses of apomixis mutants, transgenic abla ...
Sequential Elimination of Major-Effect Contributors Identifies
... Brem et al. 2002; Wang et al. 2003; Flint et al. 2005; Keurentjes et al. 2007). Although mapping strategies have been designed to detect minor-effect loci (Darvasi 1998; Satagopan et al. 2007), many of these strategies have had poor success rates, due to the confounding effects of major loci and epi ...
... Brem et al. 2002; Wang et al. 2003; Flint et al. 2005; Keurentjes et al. 2007). Although mapping strategies have been designed to detect minor-effect loci (Darvasi 1998; Satagopan et al. 2007), many of these strategies have had poor success rates, due to the confounding effects of major loci and epi ...
7nQ Jj I f NO "7^07 - UNT Digital Library
... During the course of my thesis work, I was required to read a genetics textbook, with which I had not previously read. As I read this book, I realized that there was still much information to which I had not previously been exposed. My thesis work offered a new way for me obtain knowledge that I di ...
... During the course of my thesis work, I was required to read a genetics textbook, with which I had not previously read. As I read this book, I realized that there was still much information to which I had not previously been exposed. My thesis work offered a new way for me obtain knowledge that I di ...
Variation 3.3
... Calculate the amount of variation in the DNA in the beta globin gene between person A and person B. If you need help, use the example below as a guide. 1. How many bases are different between the sequence shown for person A and the sequence shown for person B? _______ How many total bases are in the ...
... Calculate the amount of variation in the DNA in the beta globin gene between person A and person B. If you need help, use the example below as a guide. 1. How many bases are different between the sequence shown for person A and the sequence shown for person B? _______ How many total bases are in the ...
Natural Selection, Infectious Transfer and the Existence Conditions
... costs on the entire bacterial population. Summers (1996) likens the wide array of genes carried on plasmids to a genetic “lending library,” arguing that plasmids are useful because they need not be maintained in every individual in the population. Though appealing as an analogy, this is not intended ...
... costs on the entire bacterial population. Summers (1996) likens the wide array of genes carried on plasmids to a genetic “lending library,” arguing that plasmids are useful because they need not be maintained in every individual in the population. Though appealing as an analogy, this is not intended ...
Genetic Recombination in Pseudomonas aeruginosa
... then this could result from a fusion mechanism similar to that in Escherichia coli, by a transforming principle as in the pneumococcus, by an infectious process as in salmonella, or through some previously unencountered mechanism. For several reasons the fusion mechanism is thought to be the most li ...
... then this could result from a fusion mechanism similar to that in Escherichia coli, by a transforming principle as in the pneumococcus, by an infectious process as in salmonella, or through some previously unencountered mechanism. For several reasons the fusion mechanism is thought to be the most li ...
1 - CiteSeerX
... cytosine has not yet been found in any microorganism or virus. Thus a new pyrimidine base has been found in several viruses which has not yet been found in the bacterial host or its nucleic acids. It is conceivable that HMC might be present in trace amounts in E. coli, but the substance has not yet ...
... cytosine has not yet been found in any microorganism or virus. Thus a new pyrimidine base has been found in several viruses which has not yet been found in the bacterial host or its nucleic acids. It is conceivable that HMC might be present in trace amounts in E. coli, but the substance has not yet ...
![8-chromo_struct variation [Autosaved]](http://s1.studyres.com/store/data/005250938_1-5f6563dd4a633df2b3256cdb1328b758-300x300.png)
Morgan and Linkage
... two schematic sperm in panel D). As a result, the offspring usually inherits a combination of a parents paternal and maternal chromosomes. The probability that a recombination event occurs between two loci is a function of the distance between the two loci. The alleles at two loci that are far apart ...
... two schematic sperm in panel D). As a result, the offspring usually inherits a combination of a parents paternal and maternal chromosomes. The probability that a recombination event occurs between two loci is a function of the distance between the two loci. The alleles at two loci that are far apart ...
Replication timing as an epigenetic mark
... segments that replicate less than two hours apart, remain distinct and retain their size and intensity for multiple generations.16,17 Intriguingly, the entire cohort of foci replicated simultaneously in one short time interval have been observed to replicate in almost perfect synchrony at the same t ...
... segments that replicate less than two hours apart, remain distinct and retain their size and intensity for multiple generations.16,17 Intriguingly, the entire cohort of foci replicated simultaneously in one short time interval have been observed to replicate in almost perfect synchrony at the same t ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
... identified using restriction site polymorphisms introduced by the alanine codon substitutions (often involving the enzyme Fnu4H1, see Figure 4). Dideoxy-chain termination DNA sequencing (SANGER, NICKLEN and COULSON1977) was used to confirm replacements that did not alter plasmid restriction sites. A ...
... identified using restriction site polymorphisms introduced by the alanine codon substitutions (often involving the enzyme Fnu4H1, see Figure 4). Dideoxy-chain termination DNA sequencing (SANGER, NICKLEN and COULSON1977) was used to confirm replacements that did not alter plasmid restriction sites. A ...
$doc.title
... 1998; Iroskie et aI., 1999), AFLPs (Zabeau & Vos, 1993; Lottering et ai., 1999) and SIS markers (Gupta et ai., 1998; Naik et al., 1998; Seyfarth et al., 1999; Lottering et ...
... 1998; Iroskie et aI., 1999), AFLPs (Zabeau & Vos, 1993; Lottering et ai., 1999) and SIS markers (Gupta et ai., 1998; Naik et al., 1998; Seyfarth et al., 1999; Lottering et ...
New lysosomal acid lipase gene mutants explain the phenotype of
... three patients were first identified through nucleotide sequencing of single clones derived form RT/PCR of LAL mRNA. The presence of each mutation in genomic DNA and the pattern of inheritance in each family were then analyzed by direct sequencing of genomic DNA (Fig. 1). Genetic analysis of exon 10 ...
... three patients were first identified through nucleotide sequencing of single clones derived form RT/PCR of LAL mRNA. The presence of each mutation in genomic DNA and the pattern of inheritance in each family were then analyzed by direct sequencing of genomic DNA (Fig. 1). Genetic analysis of exon 10 ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... presence of the inducer Tet (13). For the episomal allele, the analysis was carried in the presence of streptomycin (Sm), conditions in which conjugal transfer to scavenger cells is required for detection of reversion (10), because the lacI33carrying cells are sensitive to Sm and the scavenger cells ...
... presence of the inducer Tet (13). For the episomal allele, the analysis was carried in the presence of streptomycin (Sm), conditions in which conjugal transfer to scavenger cells is required for detection of reversion (10), because the lacI33carrying cells are sensitive to Sm and the scavenger cells ...
A viability-linked metagenomic analysis of
... thought to be strongly influenced by the human microbiome. The cleanroom itself has previously been posited as representing an extreme environment [17], characterized by rigorous cleaning and bioburden control regimens, controlled humidity (45 ± 5 %) and temperature (25° C), and a paucity of availab ...
... thought to be strongly influenced by the human microbiome. The cleanroom itself has previously been posited as representing an extreme environment [17], characterized by rigorous cleaning and bioburden control regimens, controlled humidity (45 ± 5 %) and temperature (25° C), and a paucity of availab ...
The Mycobacterium tuberculosis katG promoter region contains a
... with these show similarity to previously identified mycobacterial promoters. Deletions in the promoter fragment, introduced with BAL-31 nuclease and restriction endonucleases, revealed that a region between 559 and 448 bp upstream of the translation initiation codon, designated the upstream activato ...
... with these show similarity to previously identified mycobacterial promoters. Deletions in the promoter fragment, introduced with BAL-31 nuclease and restriction endonucleases, revealed that a region between 559 and 448 bp upstream of the translation initiation codon, designated the upstream activato ...
Plant speciation through chromosome instability and ploidy change
... Multivalent: Physical linkage of three or more chromosomes at the meiotic diakinesis-metaphase I stages through establishment of genetic cross-overs or chiasmata. Neo-functionalization: The acquisition of novel function in one of a pair of genes resulting from a gene or a whole genome duplication ev ...
... Multivalent: Physical linkage of three or more chromosomes at the meiotic diakinesis-metaphase I stages through establishment of genetic cross-overs or chiasmata. Neo-functionalization: The acquisition of novel function in one of a pair of genes resulting from a gene or a whole genome duplication ev ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.