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Differential Gene Expression in the Siphonophore
Differential Gene Expression in the Siphonophore

Silene sex chromosome genetic map, p. 1 Expansion of
Silene sex chromosome genetic map, p. 1 Expansion of

... repetitive sequence content (Hobza et al. 2007; Cermak et al. 2008; Macas et al. 2008). Moreover, sequencing alone does not identify the non-recombining regions of the sex chromosomes. To distinguish these regions from the recombining pseudoautosomal region (PAR), it is necessary also to show that v ...
Distinct functions of two olfactory marker protein genes derived from
Distinct functions of two olfactory marker protein genes derived from

to the complete text - David Moore`s World of Fungi
to the complete text - David Moore`s World of Fungi

... outset, that these genes are on different chromosomes. A consequence of this is that if we cross two unknown genes together and get a close to 50% recombinant genotypes we can only describe them as showing ‘random assortment’ or ‘random segregation’. The two genes might be on different chromosomes a ...
Towards an accurate identification of mosaic genes and partial
Towards an accurate identification of mosaic genes and partial

... recombined segments can be derived from other strains of the same species or from other more distant bacterial or viral relatives (7,9). When the incoming DNA is significantly different from the host DNA, mosaic genes can express proteins with novel phenotypes (e.g. in the case when the donor DNA der ...
video slide - Dublin City Schools Home
video slide - Dublin City Schools Home

... • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This collection is called a genomic library. ...
Blankety Blank - misslongscience
Blankety Blank - misslongscience

... Blankety Blank 2. A gene is a sequence of nucleotides along a piece of DNA that determines a single characteristic of an organism. It does this by coding for particular polypeptides that make up the enzymes needed in a biochemical pathway. ...
生物信息学主要英文术语及释义
生物信息学主要英文术语及释义

... Population of identical cells or molecules (e.g. DNA), derived from a single ancestor. Cloning Vector (克隆载体) A molecule that carries a foreign gene into a host, and allows/facilitates the multiplication of that gene in a host. When sequencing a gene that has been cloned using a cloning vector (rathe ...
Supplemental Material
Supplemental Material

... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
controlling flowering time and plant height in
controlling flowering time and plant height in

... et a!. (1993). However, in many cases the traits vary quantitatively suggesting that they are controlled by many genes. Molecular marker maps, usually based on RFLPs, have been widely used to map quantitative trait loci ...
Predicting tRNA and tmRNA genes Aragorn - SEA
Predicting tRNA and tmRNA genes Aragorn - SEA

... shortened stem, or be missing a specific conserved base. Any of these flaws may prevent Aragorn from identifying them. However, it is possible to relax the parameters in tRNAscan-SE in order to identify them in our genomes. We have empirically determined the settings we think are appropriate for ide ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides

Predicting_tRNA_and_tmRNA_genes_12-2-16
Predicting_tRNA_and_tmRNA_genes_12-2-16

Molecular Signatures of Natural Selection for Polymorphic Genes of
Molecular Signatures of Natural Selection for Polymorphic Genes of

... A large body of research has examined the behavioral and mental health consequences of polymorphisms in genes of the dopaminergic and serotonergic systems. Along with this, there has been considerable interest in the possibility that these polymorphisms have developed and/or been maintained due to t ...
Author`s personal copy - Real Jardín Botánico
Author`s personal copy - Real Jardín Botánico

... or fitness. Both types can even coexist in some groups (e.g. arthropods, ascomycetes, alveolates, etc.). However, generally only one or two species were studied on behalf of an entire phylum. For green plants, including the chlorophyte algae lineages, angiosperms are commonly chosen as representative ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... plasmid than with the chrA gene alone (Fig. 1a). This increased chromate resistance conferred by the complete operon was more pronounced under low-sulfate growth conditions (0.05 mM sulfate; data not shown). Because the chrAC genes conferred a resistance level similar to that of chrA alone (data not ...
Genome partitioning of genetic variation for complex traits using
Genome partitioning of genetic variation for complex traits using

First report of a tetracycline-inducible gene
First report of a tetracycline-inducible gene

... (0.22 mm) and plating onto solid medium with antibiotic. Plasmid integration into the host chromosome was checked by Southern blot hybridization of HincIIrestricted genomic DNAs from 10 individual colonies with the Gmr probe. In all 10 subclones tested, the Gmr probe did not reveal the 9.6 kbp fragm ...
The Effects of Zygotic Lethal Mutations on Female Germ
The Effects of Zygotic Lethal Mutations on Female Germ

Mutualism and asexual reproduction influence recognition genes in a fungal... M.A.  van  der  Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A. van der Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*

... (dS) substitution rate ratio (Yang and Nielson, 1998). To test for variation of selective pressures across the codons, goodness of fit was calculated for the different site-specific models proposed by Yang et al. (2000). Statistical significance was calculated with likelihood ratio tests (LRT), whic ...
pdf
pdf

... strains. Using functional genes is an alternative approach to investigate denitrifying organisms in the environment. Nitrite reductase genes have been successfully detected by gene probe analysis and direct polymerase chain reaction (PCR) ampli¢cation with gene-speci¢c primers [5^12]. More than 200 ...
The Nucleotide Sequence Determination of Catalases of Three
The Nucleotide Sequence Determination of Catalases of Three

Homologous Recombination Generates T-Loop
Homologous Recombination Generates T-Loop

... Figure 4. XRCC3 and NBS1 Are Required for TRF2⌬B-Induced Telomere Loss (A) Requirement for XRCC3. Telomeric signals in human HCT116 cells, XRCC3⫺/⫺ HCT116 cells, and XRCC3⫺/⫺ HCT116 cells expressing XRCC3 from exogenous cDNA infected with TRF2⌬B retrovirus or a vector control and harvested on day 5 ...
Functional Annotation
Functional Annotation

... Bio-ontologies are used to capture biological information in a way that can be read by both humans and computers.  necessary for high-throughput “omics” datasets  allows data sharing across databases ...
Extensive tRNA gene changes in synthetic Brassica
Extensive tRNA gene changes in synthetic Brassica

... codon of mRNA. tRNA molecules with specific anticodons are known as isoacceptors, and read different codons to produce the same amino acid. There are 21 isoacceptor families, 20 for standard amino acids and one for selenocysteine (Goodenbour and Pan 2006). The secondary structure of tRNA consists of ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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