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meiosis
meiosis

... Telophase II – The only difference from mitotic division is that chromosomes do not replicate before they divide at centromeres. ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
Translation of Cyclin mRNA Is Necessary for Extracts of Activated

... comparison of the sequences of clones obtained from different libraries (from different frog colonies) showed a significant number of nucleotide substitutions. In every case, however, the differences were at third-base positions and did not alter the predicted amino acid sequences. Figure 2A compare ...
Chromosomal Basis of Inherited Disorders
Chromosomal Basis of Inherited Disorders

... pericentric inversion in chromosome 18 appears to have contributed to the evolution of humans. This inversion is not present in our closest genetic relatives, the chimpanzees. Humans and chimpanzees dier cytogenetically by pericentric inversions on several chromosomes and by the fusion of two separ ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria

Recombination and clonal groupings within Helicobacter pylori from
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The sequence of human serum albumin cDNA and its expression in

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slides giving some background and describing the

... induce several important changes in the MS/MS spectrum: – Precursor m/z is shifted by /z – All the m/z values of the fragment ions, which contain the modified amino acid are shifted by /z – All the m/z values of the fragment ions, which do not contain the modified amino acid remain the same. ...
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The physics behind the larger scale organization of DNA in eukaryotes
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The Evolution of Vertebrate Sex Chromosomes

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Amino acid alterations essential for increasing the catalytic activity of

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Gene Technology Regulation 2002
Gene Technology Regulation 2002

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... 2. Vehicles and Replicators “A vehicle is any unit, discrete enough to seem worth naming, which houses a collection of replicators and which works as a unit for the preservation and propagation of those replicators”, Richard Dawkins wrote in Extended Phenotype. Dawkins utilized the concepts of repli ...
CHROMOSOMES AND DISEASE
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... Types of Chromosomal Abnormalities A chromosomal abnormality can be a numerical deviation from the diploid number (46, XX or 46, XY) or a structural rearrangement such as an inversion, translocation or deletion. The abnormalities may involve one or more than one autosome, sex chromosomes or both. Mo ...
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... biochemical and molecular studies that have shown that cytosolic glycolysis/gluconeogenesis is lacking in chlorophytes (Schnarrenberger et al. 1990, 1994). In contrast, genomes of diatoms and land plants contain three FBP genes while rhodophytes harbor up to four different copies. Phylogenetic Analy ...
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Genetic Transformation of Poinsettia (Euphórbia
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... chimera plants with the L1-layer colourless (pink), L1 and L2 colourless (marbled) or all three cell layers colourless (white) (Preil, W 1994). A light pink variety of poinsettia, ‘Princettia’, is already promoted as an autumn plant (Ladstein, pers. comm.). As poinsettia is a traditional Christmas p ...
the association of chloroplast dna with photosynthetic membrane
the association of chloroplast dna with photosynthetic membrane

... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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