• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Sequence variation in the dihydrofolate reductase
Sequence variation in the dihydrofolate reductase

Equilibrium Statistics of Channel-confined DNA
Equilibrium Statistics of Channel-confined DNA

... and visualising single DNA molecules have been developed by biologists. Similarly, the development of clean room patterning techniques allows for the manufacturing of well-defined geometries down to the nanometre scale [2, 5, 17]. This has enabled the testing of old polymer theory predictions [18–23 ...
A wide-range phylogenetic analysis of Zic proteins: Implications for
A wide-range phylogenetic analysis of Zic proteins: Implications for

... genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron boundaries are not conserved between Eumetazoa and cnidarians. Therefore, we postulate that the earliest Zic genes may n ...
Genome-wide search for signatures of selection in three
Genome-wide search for signatures of selection in three

Quantitative Trait Analysis with Merlin and QTDT
Quantitative Trait Analysis with Merlin and QTDT

... Susceptibility to human disease Phenotypic variation in the population Individuals sharing these variants will be more similar for traits they control ...
Calculation of IBD probabilities
Calculation of IBD probabilities

alignable - gobics.de: Department of Bioinformatics
alignable - gobics.de: Department of Bioinformatics

... Fragment-based alignment approach (DIALIGN) useful for alignment of genomic sequences. ...
Molecular insights into mitochondrial transcription and its
Molecular insights into mitochondrial transcription and its

... that invaded an early eukaryotic cell and kept living inside this cell in a symbiotic relationship with its host (Gray et al., 1999). One of the best evidences of this theory is the fact that mitochondria contain a separate genome (mtDNA) (Nass and Nass, 1963a; Nass and Nass, 1963b), derived from th ...
IlllllllllllllIlllllllIllllllllllllllIlllllllllllllllllllllllllllllllllllll
IlllllllllllllIlllllllIllllllllllllllIlllllllllllllllllllllllllllllllllllll

... associated with lactose. This means that synthesis of lac FIG. 3 -pMYC467, a tac promoted toxin-containing operon ZYA gene products in these constructs plasmid The tac promoted toxin gene found in pMYC436 40 (pMYC47l, pMYC485, and pMYC1l6l) is constitutive and occurs independently of the concentrati ...
Standard
Standard

... those regulating Arabidopsis FT (Putterill et al., 2013). For example, LD induction in Medicago results in expression of FTa1 transcript throughout the day–night cycle. This is unlike FT which has a diurnal cycle with a peak in the late afternoon (Laurie et al., 2011; Andres and Coupland, 2012). The ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
htp Pseudomonas aeruginosa AMANDA SWANEPOEL

... htp gene cluster thought to encode proteins involved in the synthesis, assembly and export of these pili. To determine the transcriptional organization of this gene cluster, total RNA from P. aeruginosa PAO1 was analyzed by reverse transcriptase-polymerase chain reaction (RTPCR). Primers designed to ...
Meiosis - TeacherWeb
Meiosis - TeacherWeb

... • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males - in most species they contribute little to rearing offspring. ...
PowerPoint Presentation - Meiosis
PowerPoint Presentation - Meiosis

... • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males are an expensive luxury - in most species they contribute little to rearing offspring. ...
Regulation of Bacterial Conjugation in Microaerobiosis by
Regulation of Bacterial Conjugation in Microaerobiosis by

... the boundaries of the Tn10dCm element were sequenced (Torreblanca et al. 1999). Primers for chromosome walking and serial DNA sequencing were designed on the basis of such sequences. The identification of point mutations was achieved by DNA sequence alignment, using the LT2 genome database (McClella ...
A High Density Integrated Genetic Linkage Map of Soybean and the
A High Density Integrated Genetic Linkage Map of Soybean and the

Molecular genetics of the extracellular lipase of
Molecular genetics of the extracellular lipase of

Questions & Answer keys Test 2 Genetic engg.
Questions & Answer keys Test 2 Genetic engg.

... Green fodder stored under anaerobic condition is known as Select one: a. Hay b. Silage c. Straw d. Roughage ...
1q21 microdeletions
1q21 microdeletions

... segments. To pair up precisely, each chromosome ‘recognises’ matching or nearmatching DNA sequences on its partner chromosome. However, throughout the chromosomes there are many DNA sequences that are so similar that it is thought that mispairing can occur. The 1q21.1 region has an extremely complex ...
1q21 microdeletions
1q21 microdeletions

Characterization of mutations induced by N-methyl-N´-nitro
Characterization of mutations induced by N-methyl-N´-nitro

... were all point mutations with single-base substitutions and no deletions, frame shifts, ...
Genome-wide characteristics of sequence coverage by next
Genome-wide characteristics of sequence coverage by next

... • Comparisons to known resources (e.g. ESTs) Trial on Rice Transcriptome: • 120 Million 75 bp single end Illumina reads – embryo • ABySS : • Number of contigs = 6, 804 • Contig length range = 38 – 2,818 [mean = 203] ...
Full-Text PDF
Full-Text PDF

... Polymerase chain reaction (PCR) is a well-known NA based amplification assay. It is generally performed in centralised labs with expert users and specialized equipment, although there are several examples of portable systems that can be used in the field. Multiplexed PCR assays for simultaneous dete ...
Multiple Roles of the Y Chromosome in the Biology of
Multiple Roles of the Y Chromosome in the Biology of

... It is generally accepted that the sex chromosomes of most animals arose from a couple of homologous autosomes after the acquisition of a dominant sex determination gene[17,18] and that the Y chromosome is the result of the degeneration of one of them[19,20,21]. Its degeneration would be a consequenc ...
Competing Interests - Saudi Medical Journal
Competing Interests - Saudi Medical Journal

Analysis of acid-induced asr gene promoter of Enterobacteriaceae
Analysis of acid-induced asr gene promoter of Enterobacteriaceae

< 1 ... 10 11 12 13 14 15 16 17 18 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report