DNA sequencing: graph theory
... • 1988: SBH suggested as an an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. • 1994: Affymetrix develops first 64-kb DNA microarray ...
... • 1988: SBH suggested as an an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. • 1994: Affymetrix develops first 64-kb DNA microarray ...
Phylogenetic Relationships between the Western Aster Yellows
... in the 16s rRNA genes of the X-MLO, an MLO that infects Oenothera spp. (0-MLO) (25), and many other mollicutes and bacteria (5, 39). A PstI site that is often present just downstream from the EcoRI site in many acholeplasma, spiroplasma, and mycoplasma 16s rRNA genes (4) is not present in the SAY-ML ...
... in the 16s rRNA genes of the X-MLO, an MLO that infects Oenothera spp. (0-MLO) (25), and many other mollicutes and bacteria (5, 39). A PstI site that is often present just downstream from the EcoRI site in many acholeplasma, spiroplasma, and mycoplasma 16s rRNA genes (4) is not present in the SAY-ML ...
view - Association for Computational Linguistics
... the protein-producing ribosome, the double helix is “unzipped”, separating the ladder at the rungs, and a copy of the exposed DNA sequence called an mRNA transcript is synthesized, pairing in the same way the DNA did, with the exception that adenine in the DNA strand pairs with uracil (U) in the mRN ...
... the protein-producing ribosome, the double helix is “unzipped”, separating the ladder at the rungs, and a copy of the exposed DNA sequence called an mRNA transcript is synthesized, pairing in the same way the DNA did, with the exception that adenine in the DNA strand pairs with uracil (U) in the mRN ...
Wolbachia–host interactions: connecting phenotype to genotype
... Whereas our understanding of the phenotypic outcomes of Wolbachia infection is rapidly expanding, our knowledge of the molecular mechanisms that mediate these outcomes is very rudimentary. A key step forward has been the recent sequencing of two complete Wolbachia genomes, that of the wMel strain th ...
... Whereas our understanding of the phenotypic outcomes of Wolbachia infection is rapidly expanding, our knowledge of the molecular mechanisms that mediate these outcomes is very rudimentary. A key step forward has been the recent sequencing of two complete Wolbachia genomes, that of the wMel strain th ...
Bioinformatics
... • (Molecular) Bio - informatics • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize ...
... • (Molecular) Bio - informatics • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize ...
Couchioplanes caeruleus - International Journal of Systematic and
... strains were used: Actinoplanes caeruleus I F 0 13939T (IFO, Institute for Fermentation, Osaka, Japan) (T = type strain), “Actinoplanes azureus” IF0 13993T,and strain RA 335. Strain RA 335 was isolated from soil in Shiga Prefecture, Japan, on medium containing 1.0% soluble starch, 0.1% casein, 0.05% ...
... strains were used: Actinoplanes caeruleus I F 0 13939T (IFO, Institute for Fermentation, Osaka, Japan) (T = type strain), “Actinoplanes azureus” IF0 13993T,and strain RA 335. Strain RA 335 was isolated from soil in Shiga Prefecture, Japan, on medium containing 1.0% soluble starch, 0.1% casein, 0.05% ...
Defining characteristics of Tn5 Transposase non
... L372P was used for most of the experiments described in this manuscript. The E54K mutation enhances binding to the ES (20). The M56A mutation prevents translation of the inhibitor protein (Inh) and therefore eases Tnp purification. The L372P mutation increases flexibility of Tnp allowing the protein ...
... L372P was used for most of the experiments described in this manuscript. The E54K mutation enhances binding to the ES (20). The M56A mutation prevents translation of the inhibitor protein (Inh) and therefore eases Tnp purification. The L372P mutation increases flexibility of Tnp allowing the protein ...
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... us to further characterize the marker using FISH with a range of specific fluorescence probes, interpret the origin and occurrence of marker in the male proposita. The marker chromosome showed satellite-like appearance resembling acrocentric chromosome on either side of the centromere (bi-satellite ...
... us to further characterize the marker using FISH with a range of specific fluorescence probes, interpret the origin and occurrence of marker in the male proposita. The marker chromosome showed satellite-like appearance resembling acrocentric chromosome on either side of the centromere (bi-satellite ...
Replication timing and transcriptional control: beyond
... the late-replicating allele at random. Notably, it is almost always the early-replicating allele that is initially selected to undergo rearrangement, suggesting that replication timing is an early developmental marker for allelic exclusion in the immune system. Inactivation of the X chromosome is cu ...
... the late-replicating allele at random. Notably, it is almost always the early-replicating allele that is initially selected to undergo rearrangement, suggesting that replication timing is an early developmental marker for allelic exclusion in the immune system. Inactivation of the X chromosome is cu ...
Ensembl variation resources Open Access Database
... Full list of author information is available at the end of the article ...
... Full list of author information is available at the end of the article ...
NCBI Molecular Biology Resources
... COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M17755.2 and AW874082.1. On Feb 25, 2003 this sequence version replaced gi:21361188. ...
... COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M17755.2 and AW874082.1. On Feb 25, 2003 this sequence version replaced gi:21361188. ...
Single-stranded heteroduplex intermediates in l Red homologous
... recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermediates to establish single stranded heteroduplexes at the replication fork. We created asymmetrically dige ...
... recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermediates to establish single stranded heteroduplexes at the replication fork. We created asymmetrically dige ...
Press release
... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
Selection for TnlO Tet Repressor Binding to tet Operator
... We have constructed a genetic assay which selects positively for a functional interaction between Tet repressor and its cognate operator in Escherichia coli. In this strain Tet repressor blocks expression of lacl and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be ...
... We have constructed a genetic assay which selects positively for a functional interaction between Tet repressor and its cognate operator in Escherichia coli. In this strain Tet repressor blocks expression of lacl and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be ...
Characterization of lysine decarboxylase
... locus in Escherichia coli. Inactivation of cadC by an insertion sequence has been reported to also cause LDC negativity in E. coli (Casalino et al., 2003). PCR was used to detect the cadBA operon and the cadC gene of the LDC-negative strains. The results showed that bands with the expected sizes wer ...
... locus in Escherichia coli. Inactivation of cadC by an insertion sequence has been reported to also cause LDC negativity in E. coli (Casalino et al., 2003). PCR was used to detect the cadBA operon and the cadC gene of the LDC-negative strains. The results showed that bands with the expected sizes wer ...
Similarity Searches on Sequence Databases: BLAST
... composition of any sequence is the same as the average composition of the whole database. • However this assumption doesn’t hold all the time, some sequences have biased compositions, e.g. many proteins contain patches known as low-complexity regions: such as segments that contain many prolines or g ...
... composition of any sequence is the same as the average composition of the whole database. • However this assumption doesn’t hold all the time, some sequences have biased compositions, e.g. many proteins contain patches known as low-complexity regions: such as segments that contain many prolines or g ...
Detection of GM Papaya Event 55-1 in Fresh
... the more than 130 GMOs developed in other countries have been approved in Korea [Kim et al., 2010]. Therefore, it is necessary to develop effective GMO detection methods to monitor and identify approved as well as unapproved GMOs. In this study, we designed a set of primers to amplify an endogenous ...
... the more than 130 GMOs developed in other countries have been approved in Korea [Kim et al., 2010]. Therefore, it is necessary to develop effective GMO detection methods to monitor and identify approved as well as unapproved GMOs. In this study, we designed a set of primers to amplify an endogenous ...
Classification of Centers for Disease Control Group Eugonic
... LMG 23009 differed by two bases from the corresponding fragment of the other two strains (data not shown). Finally, the 452-base fragments of the 16S rRNA gene sequence of strains LMG 23013 and LMG 23014 were 100 % similar to the corresponding fragment of strain LMG 23012T (data not shown). In order ...
... LMG 23009 differed by two bases from the corresponding fragment of the other two strains (data not shown). Finally, the 452-base fragments of the 16S rRNA gene sequence of strains LMG 23013 and LMG 23014 were 100 % similar to the corresponding fragment of strain LMG 23012T (data not shown). In order ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.