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CBSE XII BIO QS with key(2009-2015)
CBSE XII BIO QS with key(2009-2015)

... the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual reproduction producing seeds without fertilization/ t ...
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Bio CET 2015 Key Answers

An Investigation of Acetobacter aceti N5
An Investigation of Acetobacter aceti N5

... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
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The SRA gen - uri=dna.kdna.ucla

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Folie 1 - NETTAB

... Hospital Bonn participated in the Boston Children’s Hospital’s CLARITY challenge. ...
Identification of a Vinyl Reductase Gene for
Identification of a Vinyl Reductase Gene for

... Chlorophyll metabolism has been extensively studied with various organisms, and almost all of the chlorophyll biosynthetic genes have been identified in higher plants. However, only the gene for 3,8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for monovinyl chlorophy ...
illuminaHumanv3.db October 28, 2014
illuminaHumanv3.db October 28, 2014

... indicates the chromosome. Due to inconsistencies that may exist at the time the object was built, these vectors may contain more than one chromosome and/or location. If the chromosomal location is unknown, the vector will contain an NA. Chromosomal locations on both the sense and antisense strands a ...
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... properties such as lignin content and composition. Paper quality can also be improved by changing wood properties such as microfibril angles. However, our understanding of the wood formation process is far from complete. This is an obstacle for us to genetically engineer wood properties of Eucalyptu ...
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DNA cytosine methylation in plant development

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... was then introduced into pGem7zf(⫹) (Invitrogen Inc.) with EcoRI and BamHI cleavage sites. The resulting plasmid was digested with the NarI restriction enzyme and was then ligated with the PCR product containing the kanamycin resistance gene that was amplified from pKD4 (9) by using the Kd4kanF and ...
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Divergent selection and heterogeneous genomic

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A genome-wide association scan in pig identifies novel regions

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... basis of this mechanism is not clear, Messmer et al. (2000) hypothesized that expression of Lr34 might alter the physiology of the flag leaf thus making it less desirable for the pathogen to grow and establish itself. Although Lr34/Ltn1 was the first gene to be associated with LTN, the genes Lr46/Lt ...
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Distribution of orphan metabolic activities

... technologies, has revolutionized biological research [1,2]. The race to obtain complete functional datasets for various model organisms is under way [3]. The best known example is the exponential growth of complete genomic sequences (http://www.genomesonline.org/). There are multiple ongoing efforts ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
Binding of Hoechst with nucleic acids using fluorescence spectroscopy

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Nerve activates contraction

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hag expression in Bacillus subtilis is both negatively

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... Cattle, especially the young ones, have been implicated as a principal source for E. coli O157:H7 [4]. Most infections caused by E. coli O157: H7 result from the consumption of contaminated and undercooked ground beef and unpasteurized fruit juices. Dairy products, cheese from raw milk [1,25]. Virul ...
Adaptation of Drosophila to a novel laboratory environment reveals
Adaptation of Drosophila to a novel laboratory environment reveals

... multiple generations and, as in nature, fitness differences among individuals result in adaptation. Most experimental evolution studies in Drosophila have focused on the response of various phenotypic traits to selection. Thus, despite a large number of studies, very little is known about the underl ...
The Mitochondrial Genome of Chara vulgaris
The Mitochondrial Genome of Chara vulgaris

... mtDNA sequences of Mesostigma (Turmel et al., 2002b), of the charophycean alga Chaetosphaeridium globosum (Coleochaetales) (Turmel et al., 2002c), of the bryophyte Marchantia polymorpha (a liverwort) (Oda et al., 1992a), and of the angiosperms Arabidopsis (Unseld et al., 1997), sugar beet (Kubo et a ...
Graph-based Methods for the Design of DNA - TUBdok
Graph-based Methods for the Design of DNA - TUBdok

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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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