The virulence plasmids of Salmonella
... that they are necessary, but not sufficient, to induce fluid secretion in the suckling murine model. Adhesion mediated by PEF is different from that induced by the chromosome-encoded lpf (long polar fimbriae), which promote the adhesion of Salmonella to Peyer’s patches [9]. However, PEF do not media ...
... that they are necessary, but not sufficient, to induce fluid secretion in the suckling murine model. Adhesion mediated by PEF is different from that induced by the chromosome-encoded lpf (long polar fimbriae), which promote the adhesion of Salmonella to Peyer’s patches [9]. However, PEF do not media ...
Costimulatory receptors in jawed vertebrates: Conserved
... (Tetraodon nigroviridis) and zebrafish (Danio rerio). In zebrafish, it was not possible to find sequences corresponding to exons 3 and 4 (encoding transmembrane and intracytoplasmic region) from the available genome assembly. The Ensembl zebrafish genome assembly (v36) had two copies of CD28 exons 1 and ...
... (Tetraodon nigroviridis) and zebrafish (Danio rerio). In zebrafish, it was not possible to find sequences corresponding to exons 3 and 4 (encoding transmembrane and intracytoplasmic region) from the available genome assembly. The Ensembl zebrafish genome assembly (v36) had two copies of CD28 exons 1 and ...
Prokaryotic features of a nucleus
... puzzling contrast with a previous publication demonstrating that Escherichia coli GAPDH is relatively similar to the eukaryotic enzymes [Eur. J. Biochem. 150,61-66 (1985)l. It is now well established that plastid and eubacterial genes share a high degree of sequence homology [I - 61 in agreement wit ...
... puzzling contrast with a previous publication demonstrating that Escherichia coli GAPDH is relatively similar to the eukaryotic enzymes [Eur. J. Biochem. 150,61-66 (1985)l. It is now well established that plastid and eubacterial genes share a high degree of sequence homology [I - 61 in agreement wit ...
Characterisation of interstitial duplications and triplications of
... showed that, in all cases, the duplications and triplications involved the PWACR and were not pseudogene expansions. Retrospective cytogenetic analysis in families 7 and 13 did not identify these duplications clearly. The size of the pericentromeric area of 15q varies greatly within the normal popul ...
... showed that, in all cases, the duplications and triplications involved the PWACR and were not pseudogene expansions. Retrospective cytogenetic analysis in families 7 and 13 did not identify these duplications clearly. The size of the pericentromeric area of 15q varies greatly within the normal popul ...
Genetic Homologies between Flagellar Antigens of
... Hybrids of Salmonella abony and Escherichia coli combining antigenic characteristics of both parents have been obtained. In this case the antigens, each determined by distinct structural genes, retain their original identity. Only flagellar antigens have so far been studied, and these strains would ...
... Hybrids of Salmonella abony and Escherichia coli combining antigenic characteristics of both parents have been obtained. In this case the antigens, each determined by distinct structural genes, retain their original identity. Only flagellar antigens have so far been studied, and these strains would ...
The Nicotiana tabacum genome encodes two cytoplasmic
... constructed with the second fraction (3.4-4.9 kb) by cloning in predigested lambda, ZAPII-EcoRI (Stratagene) and packaging with a Gigapack Gold packaging kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombin ...
... constructed with the second fraction (3.4-4.9 kb) by cloning in predigested lambda, ZAPII-EcoRI (Stratagene) and packaging with a Gigapack Gold packaging kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombin ...
EXCHANGE OF GENETIC MATERIAL BETWEEN
... Hfr character, unlike the F+ character, is not transferable to F- cells by infection but can be transferred by conjugation (BERNSTEIN1958); in other words, the factor responsible for Hfr is located on the bacterial chromosome. An attempt was therefore made to introduce the Hfr character into Salmone ...
... Hfr character, unlike the F+ character, is not transferable to F- cells by infection but can be transferred by conjugation (BERNSTEIN1958); in other words, the factor responsible for Hfr is located on the bacterial chromosome. An attempt was therefore made to introduce the Hfr character into Salmone ...
Extended spectrum beta-lactamases - Micro-Rao
... beta-lactams produced by environmental bacteria and fungi. First detection of penicillinase in gram positive bacteria (Staphylococcus aureus) was reported in 1944. Initially, the genes coding for betalactamases were found in bacterial chromosomes. These enzymes were inducible and constitutively expr ...
... beta-lactams produced by environmental bacteria and fungi. First detection of penicillinase in gram positive bacteria (Staphylococcus aureus) was reported in 1944. Initially, the genes coding for betalactamases were found in bacterial chromosomes. These enzymes were inducible and constitutively expr ...
2. Assessing the probative value of DNA evidence
... Well-informed observers have for many decades been arguing the case for making basic training in probability and statistics an integral component of legal education (e.g. Kaye, 1984). But little tangible progress has been made. It is sometimes claimed that lawyers and the public at large fear anythi ...
... Well-informed observers have for many decades been arguing the case for making basic training in probability and statistics an integral component of legal education (e.g. Kaye, 1984). But little tangible progress has been made. It is sometimes claimed that lawyers and the public at large fear anythi ...
Assessing the Probative Value of DNA Evidence
... Well-informed observers have for many decades been arguing the case for making basic training in probability and statistics an integral component of legal education (e.g. Kaye, 1984). But little tangible progress has been made. It is sometimes claimed that lawyers and the public at large fear anythi ...
... Well-informed observers have for many decades been arguing the case for making basic training in probability and statistics an integral component of legal education (e.g. Kaye, 1984). But little tangible progress has been made. It is sometimes claimed that lawyers and the public at large fear anythi ...
1 Article: Investigation Evidence for Stabilizing Selection on Codon
... The third chromosome of D. pseudoobscura is highly polymorphic for different gene arrangements that were generated through a series of >30 overlapping paracentric inversion mutations. This gene arrangement polymorphism has been stable in natural populations for over seventy years (Figure 1A; Dobzha ...
... The third chromosome of D. pseudoobscura is highly polymorphic for different gene arrangements that were generated through a series of >30 overlapping paracentric inversion mutations. This gene arrangement polymorphism has been stable in natural populations for over seventy years (Figure 1A; Dobzha ...
... embryos. 5meC lane = DNA precipitated by antibody against methylated cytosine; IgG = non-specific immunoprecipitation; Input = DNA before immunoprecipitation; - = no antibody control. Specific bands for Kcnq1 and Kcnq1ot1 are indicated; NS = non-specific amplification product. The Kcnq1ot1 promoter ...
DNA
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
Primer on Molecular Biology
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
Primer on Molecular Biology
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
... • Phenotype: the physical expressed traits of an organism • Nucleic acid: Biological molecules(RNA and DNA) that allow organisms to reproduce; ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.