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Standards and guidelines for the interpretation of sequence
Standards and guidelines for the interpretation of sequence

... that have developed variant classification guidelines for wellstudied genes in breast cancer, colon cancer, and cystic fibrosis and statistical analysis programs for quantitative evaluation of variants in select diseases.2–5 While those variant analysis guidelines are useful in a specific setting, i ...
Cloning, Sequencing, and Characterization of Luciola italica
Cloning, Sequencing, and Characterization of Luciola italica

... extension at 68 °C for 5 min. The PCR products were purified using the QIAquick PCR purification kit (Qiagen), and eluted in sterile water. Purified products were verified by 1.0% agarose gel electrophoresis. DNA sequencing of the PCR-amplified cDNA was performed with a capillary array sequencer CEQ ...
The Plant Cell
The Plant Cell

... markers from two cloned genes (ArLIM15 and ABI3) and two restriction fragment length polymorphism markers (g4711 and m255) located approximately between gl1 and axr2 (Wilson et al., 1990; Giraudat et al., 1992; Klimyuk and Jones, 1997). The m255 CAPS marker was found to be tightly linked to the alf5 ...
Cells: building blocks of living organisms
Cells: building blocks of living organisms

... The self-replicating genetic structures of cells containing the cellular DNA that bears in its nucleotide sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose D ...
Xq28 duplications
Xq28 duplications

... Your geneticist or genetic counsellor will tell you about the point(s) where your child’s chromosome has broken and the extra genetic material has been added. The information you are given will depend on the method used to test your child’s chromosomes. If the duplicated piece of DNA was identified ...
8 VARIATION IN CHROMOSOME STRUCTURE AND NUMBER
8 VARIATION IN CHROMOSOME STRUCTURE AND NUMBER

... of genetic material within a single chromosome. In Figure 8.2, human chromosomes are labeled according to their normal G-banding patterns. When a deletion occurs, a segment of chromosomal material is missing. In other words, the affected chromosome is deficient in a significant amount of genetic mat ...
Distribution and structural variation of the she pathogenicity island in
Distribution and structural variation of the she pathogenicity island in

... approach. This ®nding suggested that either major rearrangements of the genes had occurred or, as a consequence of large insertions into the int± sigA intergenic region, the int±sigA fragment was too long to be ampli®ed by PCR. To investigate the latter possibility, chromosomal DNA from each strain ...
A survey on computational methods for enhancer and
A survey on computational methods for enhancer and

... proximity in the three-dimensional genome structure but are not necessarily adjacent to each other in the primary DNA sequence, without requiring an input set of candidates. Among these two techniques, ChIA-PET further requires that a chosen factor, such as RNA polymerase II, is involved in the DNA ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is

... sequence analysis of exon VI11 showed the presence of two bands at the same migration distance, corresponding to Lys (AAC + AAA). amino acid substitution y 337 Asn The purified double-stranded PCR-derived fragment was cloned and subjected to sequence analysis. Normal and mutated alleles of fibrinoge ...
Optimality models of phage life history and parallels in
Optimality models of phage life history and parallels in

... phage; k is adsorption rate of phage to cells; b is burst size (number of phage per burst); L is the latent period, the time from infection to lysis (minutes). In contrast to the usual SIR models of infectious disease, this equation describes the free, infectious particles themselves. Infected hosts ...
Evaluation of computational metabolic
Evaluation of computational metabolic

... that reaction. (2) Enzyme names are degenerate in the sense that the biomedical literature, and databases such as Swiss–Prot and Genbank, refer to a given enzymatic activity using many different names for the same activity, and occasionally, using the same name for different enzyme activities. (3) E ...
A MIAME-compliant Microarray Database
A MIAME-compliant Microarray Database

... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
An Introduction to Genetic Analysis Chapter 18 Chromosome
An Introduction to Genetic Analysis Chapter 18 Chromosome

... Because 4 is an even number, autotetraploids can have a regular meiosis, although this is by no means always the case. The crucial factor is how the four homologous chromosomes, one from each of the four sets, pair and segregate. There are several possibilities, as shown in Figure 18-6. Pairings bet ...
LOCATION OF THE CENTROMERES ON THE LINKAGE
LOCATION OF THE CENTROMERES ON THE LINKAGE

Real-time PCR-based detection of Bordetella pertussis and
Real-time PCR-based detection of Bordetella pertussis and

Candidate gene scan for Single Nucleotide Polymorphisms involved
Candidate gene scan for Single Nucleotide Polymorphisms involved

The X Chromosome in Quantitative Trait Locus Mapping
The X Chromosome in Quantitative Trait Locus Mapping

... i fðyi j mji ; sÞ, for which MLEs may be obtained in closed form. A LOD score is calculated as the log (base 10) of the ratio of the maximized likelihoods under the alternative and the null hypotheses. Chromosome-specific thresholds: The usual approach for establishing statistical significance in a ...
Engineering of polyketide biosynthetic pathways for bioactive
Engineering of polyketide biosynthetic pathways for bioactive

Resolving Individuals Contributing Trace Amounts of DNA to Highly
Resolving Individuals Contributing Trace Amounts of DNA to Highly

- BioMedSearch
- BioMedSearch

... The purified PSRP catalysed both the ADP/ATP-dependent inactivation (Table 1) and the Pi-dependent activation of purified E. coli PEPS (Table 2). In addition, the expressed PSRP catalysed in-assay activation. In-assay activation occurs when inactivated PEPS in aliquots taken from a separate inactiva ...
Simple identification of dominant p53 mutants by
Simple identification of dominant p53 mutants by

... Such mutations, although completely inactive for transactivation (red colonies), are apparently not selected for in vivo during the carcinogenic process. This could reflect mutagen specificity, repair specificity or some biological activity of the mutants. In particular, if dominance plays an import ...
Phylogenetic analysis of three complete gap
Phylogenetic analysis of three complete gap

... from the zebrafish genome, some are found as single relatives, and others are found in multiple copies. In addition, the zebrafish has 14 apparently novel connexins, several of which arose by recent tandem duplication events. This analysis provides evidence that the connexin gene family is increasin ...
Sequence variation characteristics of D
Sequence variation characteristics of D

... Mitochondria are usually depicted as elongated cylinders with a diameter of 0.5 to I .O |.im. Time-lapse microcinematography of living cells, however, shows that mitochondria are remarkably mobile and plastic organelles, constantly changing their shape and even fijsing with one another and then sep ...
Rh phenotype prediction by DNA typing and its
Rh phenotype prediction by DNA typing and its

... 1997). No consensus has yet been achieved as to which polymorphism may be most reliable for testing of any given population. RH and particularly RHD DNA typing is much confounded by the presence of two highly homologous genes, RHCE and RHD, and the complex polymorphisms between both genes. In contra ...
The Serratia gene cluster encoding biosynthesis of the red antibiotic
The Serratia gene cluster encoding biosynthesis of the red antibiotic

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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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