Abstract
... be a viable approach to slow animal aging, and raises questions about folate supplementation. Aging in Caenorhabditis elegans - nature versus nuture (or a diet of E. coli)? The first genes extending lifespan were identified in the nematode Caenorhabditis elegans [1], and several of the metabolic pat ...
... be a viable approach to slow animal aging, and raises questions about folate supplementation. Aging in Caenorhabditis elegans - nature versus nuture (or a diet of E. coli)? The first genes extending lifespan were identified in the nematode Caenorhabditis elegans [1], and several of the metabolic pat ...
Unusual ADP-forming acetyl-coenzyme A synthetases from the
... Purification of ACD from H. marismortui H. marismortui (DSM 3752) (Oren et al. 1990) was grown aerobically in a 8-l fermentor (FairmenTec, Germany) on a medium containing glucose as carbon and energy source (for detailed description of the media, see Bräsen and Schönheit 2001). Cell extracts were p ...
... Purification of ACD from H. marismortui H. marismortui (DSM 3752) (Oren et al. 1990) was grown aerobically in a 8-l fermentor (FairmenTec, Germany) on a medium containing glucose as carbon and energy source (for detailed description of the media, see Bräsen and Schönheit 2001). Cell extracts were p ...
(From the Department of Genetics, Carnegie Institution of
... per bacterium at 25 minutes. The final burst size in diluted cultures is 30 to 40 per bacterium, reached at 50 minutes. At 2 × l0 s cells per ml., the culture lyses slowly, and yields 140 phage per bacterium. The growth of both bacteria and phage in this medium is as reproducible as that in broth. F ...
... per bacterium at 25 minutes. The final burst size in diluted cultures is 30 to 40 per bacterium, reached at 50 minutes. At 2 × l0 s cells per ml., the culture lyses slowly, and yields 140 phage per bacterium. The growth of both bacteria and phage in this medium is as reproducible as that in broth. F ...
Independent functions of viral protein and nucleic
... per bacterium at 25 minutes. The final burst size in diluted cultures is 30 to 40 per bacterium, reached at 50 minutes. At 2 × l0 s cells per ml., the culture lyses slowly, and yields 140 phage per bacterium. The growth of both bacteria and phage in this medium is as reproducible as that in broth. F ...
... per bacterium at 25 minutes. The final burst size in diluted cultures is 30 to 40 per bacterium, reached at 50 minutes. At 2 × l0 s cells per ml., the culture lyses slowly, and yields 140 phage per bacterium. The growth of both bacteria and phage in this medium is as reproducible as that in broth. F ...
Novel pathogen-specific primers for the detection of Agrobacterium
... most opine types found in grapevines. Reproducible detection of all the tested pathogens in a single reaction was only possible with multiplex PCR using mixtures of virulence-, or oncogene specific primers. A primer combination including pehA, virF and virD2 gene-specific oligonucleotides amplified the ...
... most opine types found in grapevines. Reproducible detection of all the tested pathogens in a single reaction was only possible with multiplex PCR using mixtures of virulence-, or oncogene specific primers. A primer combination including pehA, virF and virD2 gene-specific oligonucleotides amplified the ...
Specific and ubiquitous expression of different Zn finger protein
... Many eucaryotic genes involved in the process of differentiation and development are expressed in a temporally and spatially restricted fashion(15). Considering that • regulation of such genes also occur at the transcriptional level,the identification of different transcriptional regulatory factors ...
... Many eucaryotic genes involved in the process of differentiation and development are expressed in a temporally and spatially restricted fashion(15). Considering that • regulation of such genes also occur at the transcriptional level,the identification of different transcriptional regulatory factors ...
TaqMan-Based Real-Time PCR for Genotyping Common
... corresponding reports of negative associations with disease (2 ). Thus, development of a simple, highthroughput HP genotyping method is needed to facilitate these association studies. Several methods for phenotyping HP1 and HP2 have been described. High-pressure gel-permeation chromatography and ge ...
... corresponding reports of negative associations with disease (2 ). Thus, development of a simple, highthroughput HP genotyping method is needed to facilitate these association studies. Several methods for phenotyping HP1 and HP2 have been described. High-pressure gel-permeation chromatography and ge ...
Pax1, Pax9, Vertebral column, Chondrogenesis, Proliferation
... sequences flanking P-element insertions were recovered by plasmid rescue (Pirrotta, 1986). All DNA sequences were determined on both strands by the dideoxynucleotide method with a DNA sequencer Model 373A using dye terminators (Applied Biosystems Inc.). Using the 3.2, 0.6 and 3.8 kb genomic EcoRI fr ...
... sequences flanking P-element insertions were recovered by plasmid rescue (Pirrotta, 1986). All DNA sequences were determined on both strands by the dideoxynucleotide method with a DNA sequencer Model 373A using dye terminators (Applied Biosystems Inc.). Using the 3.2, 0.6 and 3.8 kb genomic EcoRI fr ...
Designing synthetic MLPA probes - MRC
... Hybridising parts of LPO and RPO. Bind to adjacent target DNA sequences. Probe oligonucleotide that is situated on the left when probe is shown from 5’ to 3’. Consists of forward PCR primer sequence (5’ end) and hybridising sequence (3’ end). 3’ nucleotide of LPO and 5’ nucleotide of RPO, where liga ...
... Hybridising parts of LPO and RPO. Bind to adjacent target DNA sequences. Probe oligonucleotide that is situated on the left when probe is shown from 5’ to 3’. Consists of forward PCR primer sequence (5’ end) and hybridising sequence (3’ end). 3’ nucleotide of LPO and 5’ nucleotide of RPO, where liga ...
GC-content of synonymous codons profoundly influences amino
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
Mapping of QTL for body conformation and behavior in cattle
... The genetic marker map covered 3155.5 cM of the bovine genome across all autosomes and the pseudoautosomal region of the sex chromosomes. The average marker interval was 13.9 cM and ranged from 6.0 cM (chromosome 23) to 23.2 cM (chromosome 9) on individual chromosomes (Table 2). A total of 60 QTL wi ...
... The genetic marker map covered 3155.5 cM of the bovine genome across all autosomes and the pseudoautosomal region of the sex chromosomes. The average marker interval was 13.9 cM and ranged from 6.0 cM (chromosome 23) to 23.2 cM (chromosome 9) on individual chromosomes (Table 2). A total of 60 QTL wi ...
The effecT of chlorinaTion of nucleoTide bases on The
... the normal vital functioning of the bacteria and its ability to divide normally, but it was found that these investigated organisms were not capable to transfer the modified fragments of DNA to the bacteria with natural DNA (containing thymine), in other words they cannot change the normal organisms ...
... the normal vital functioning of the bacteria and its ability to divide normally, but it was found that these investigated organisms were not capable to transfer the modified fragments of DNA to the bacteria with natural DNA (containing thymine), in other words they cannot change the normal organisms ...
Forche et al. 2008 PLoS Biology
... between isolates, where distinct peaks were evident representing non-replicated (G1 phase) and replicated (G2 phase) DNA. In some strains the majority of the cells contained replicated DNA (e.g., S5 and S6, Figure 2, panels N and O), while others had an almost equal distribution of cells with unrepl ...
... between isolates, where distinct peaks were evident representing non-replicated (G1 phase) and replicated (G2 phase) DNA. In some strains the majority of the cells contained replicated DNA (e.g., S5 and S6, Figure 2, panels N and O), while others had an almost equal distribution of cells with unrepl ...
The Parasexual Cycle in Candida albicans Provides an
... between isolates, where distinct peaks were evident representing non-replicated (G1 phase) and replicated (G2 phase) DNA. In some strains the majority of the cells contained replicated DNA (e.g., S5 and S6, Figure 2, panels N and O), while others had an almost equal distribution of cells with unrepl ...
... between isolates, where distinct peaks were evident representing non-replicated (G1 phase) and replicated (G2 phase) DNA. In some strains the majority of the cells contained replicated DNA (e.g., S5 and S6, Figure 2, panels N and O), while others had an almost equal distribution of cells with unrepl ...
Quantitative Analysis of the Kinetics of End
... addition, the binding of ATP or ATPgS to individual ®lament subunits can stimulate NTP hydrolysis in neighboring subunits (Lee & Cox, 1990; Menge & Bryant, 1988). These interactions tend to maintain the entire ®lament in an extended active state. However, the ATP hydrolytic cycles of adjacent monome ...
... addition, the binding of ATP or ATPgS to individual ®lament subunits can stimulate NTP hydrolysis in neighboring subunits (Lee & Cox, 1990; Menge & Bryant, 1988). These interactions tend to maintain the entire ®lament in an extended active state. However, the ATP hydrolytic cycles of adjacent monome ...
Snorks Lab File
... In this simulation, you will examine the DNA sequence of a fictitious organism - the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to analyze the genes of its DNA and determine what traits the organ ...
... In this simulation, you will examine the DNA sequence of a fictitious organism - the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to analyze the genes of its DNA and determine what traits the organ ...
Document
... In human infants, lactase is secreted in intestine which breaks the lactose into easily absorbed Glucose and Galactose. Production of the lactase enzyme declines in adults. The unabsorbed lactose creates cramps, diarrhea, and nausea. In some humans, lactase continues to be produced throughout adulth ...
... In human infants, lactase is secreted in intestine which breaks the lactose into easily absorbed Glucose and Galactose. Production of the lactase enzyme declines in adults. The unabsorbed lactose creates cramps, diarrhea, and nausea. In some humans, lactase continues to be produced throughout adulth ...
22q12 and 22q13 duplications
... cell of the body. Every chromosome contains thousands of genes which may be thought of as individual instruction booklets (or recipes) that contain all the genetic information telling the body how to develop, grow and function. Chromosomes (and genes) usually come in pairs with one member of each ch ...
... cell of the body. Every chromosome contains thousands of genes which may be thought of as individual instruction booklets (or recipes) that contain all the genetic information telling the body how to develop, grow and function. Chromosomes (and genes) usually come in pairs with one member of each ch ...
Mapping Mendelian Factors Underlying Quantitative ... Using RFLP Linkage Maps Eric
... some of the QTLs segregating in the cross have relatively large phenotypiceffects. By exploiting aclassical formula of SEWALL WRIGHT, we show that it is often possible to recognize such crosses in advance and thereby to ensure thatQTLs will in fact be identified. (ii) Exploit the full power of compl ...
... some of the QTLs segregating in the cross have relatively large phenotypiceffects. By exploiting aclassical formula of SEWALL WRIGHT, we show that it is often possible to recognize such crosses in advance and thereby to ensure thatQTLs will in fact be identified. (ii) Exploit the full power of compl ...
View/Open
... fragment, without a centromere, and a centric fragment, with a centromere. The centric fragment migrates normally during the division process because it has a centromere.The acentric fragment, however, is soon lost. It is subsequently excluded from the nuclei formed and eventually degrades. In other ...
... fragment, without a centromere, and a centric fragment, with a centromere. The centric fragment migrates normally during the division process because it has a centromere.The acentric fragment, however, is soon lost. It is subsequently excluded from the nuclei formed and eventually degrades. In other ...
The Deletion Stocks of Common Wheat
... deletion chromosome. deletion L arm tion chromosome had some chromosomal progeny for homozygous plants with deaberrations. For the production of deleletion chromosomes and the least degree The arm ratios were used to compensate of aberrations in the other chromosomes, for the difference in contracti ...
... deletion chromosome. deletion L arm tion chromosome had some chromosomal progeny for homozygous plants with deaberrations. For the production of deleletion chromosomes and the least degree The arm ratios were used to compensate of aberrations in the other chromosomes, for the difference in contracti ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.