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arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... instance, purines are larger and thus one might expect that they would interact more strongly with surfaces in a confined space. The backbone structure of a polynucleotide is shown in Fig. 3a. Each monomer unit is a nucleotide, which consists of a base, phosphate group (PO4 ), and sugar. For DNA (RN ...
... instance, purines are larger and thus one might expect that they would interact more strongly with surfaces in a confined space. The backbone structure of a polynucleotide is shown in Fig. 3a. Each monomer unit is a nucleotide, which consists of a base, phosphate group (PO4 ), and sugar. For DNA (RN ...
Studies on the key amino acid residues responsible for the alkali
... the pH optimum was detected in some active recombinants. No recombinant with a shift of the pH optimum to a more basic value was found. All of these suggested that Asn-71 had not only an important role in the catalytic ability but also an effect on the pH profile of the enzyme activity. In order to ...
... the pH optimum was detected in some active recombinants. No recombinant with a shift of the pH optimum to a more basic value was found. All of these suggested that Asn-71 had not only an important role in the catalytic ability but also an effect on the pH profile of the enzyme activity. In order to ...
Recall Questions
... Polyploidy: Gain of entire sets of chromosomes so the chromosome number changes from 2n to 3n (triploid), 4n (tetraploid), and so on. *2. Why do extra copies of genes sometimes cause drastic phenotypic effects? The expression of some genes is balanced with the expression of other genes; the ratios o ...
... Polyploidy: Gain of entire sets of chromosomes so the chromosome number changes from 2n to 3n (triploid), 4n (tetraploid), and so on. *2. Why do extra copies of genes sometimes cause drastic phenotypic effects? The expression of some genes is balanced with the expression of other genes; the ratios o ...
Identification and expression of GH
... 0377BP (Choi et al., 2004), which are classed as GH-8 chitosanases. Based on this result, two further primers (BTMO-F and BTMO-R) were designed and PCR reactions were performed with the chromosomal DNAs of all 29 B. thuringiensis strains. As summarized in Table 1, PCR products were obtained from eac ...
... 0377BP (Choi et al., 2004), which are classed as GH-8 chitosanases. Based on this result, two further primers (BTMO-F and BTMO-R) were designed and PCR reactions were performed with the chromosomal DNAs of all 29 B. thuringiensis strains. As summarized in Table 1, PCR products were obtained from eac ...
View Tutorial
... in the number of complete genomes, we are provided with the information of not only which genes are present (in order to identify the “orthologs” between different genomes) or present in multiple copies (in order to identify the “paralogs” within one genome) in any particular genome but also which o ...
... in the number of complete genomes, we are provided with the information of not only which genes are present (in order to identify the “orthologs” between different genomes) or present in multiple copies (in order to identify the “paralogs” within one genome) in any particular genome but also which o ...
thymine dimers - Glen Research
... synthesizer. Initially, we are offering the regular 4 nucleoside supports, Bz-dA, BzdC, dmf-dG and dT. In the near future, we will be adding several of our most popular supports, including RNA supports, for this platform. We are also adding 96 well plates containing both of our CPG-based universal ...
... synthesizer. Initially, we are offering the regular 4 nucleoside supports, Bz-dA, BzdC, dmf-dG and dT. In the near future, we will be adding several of our most popular supports, including RNA supports, for this platform. We are also adding 96 well plates containing both of our CPG-based universal ...
The Effect of OmpA Expression on Hfr Conjugation Efficiency
... efficiency, are only approximations. Proline auxotrophy and streptomycin resistance were used as selective markers for recipient bacteria that underwent successful conjugation with an HfrC donor. Growth of C149, C156, and C158 strains on minimal selective medium supplemented with proline was not att ...
... efficiency, are only approximations. Proline auxotrophy and streptomycin resistance were used as selective markers for recipient bacteria that underwent successful conjugation with an HfrC donor. Growth of C149, C156, and C158 strains on minimal selective medium supplemented with proline was not att ...
Genetic linkage studies in the pseudoautosomal
... marker and the disease-causing gene are said to be linked, and are assumed to be very close together. After using linkage to get an idea where risk genes may be located, association studies allow to test candidate genes, or very small genetic regions, to see if they are associated with having the di ...
... marker and the disease-causing gene are said to be linked, and are assumed to be very close together. After using linkage to get an idea where risk genes may be located, association studies allow to test candidate genes, or very small genetic regions, to see if they are associated with having the di ...
DNA Evidence: Probability, Population Genetics, and the Courts
... 7. See David H. Kaye, The Admissibility ofDNA Testing, 13 CARDOZO L. REV. 353, 357 n.17 (1991). A case that is representative of this epoch is Cobey v. State, 559 A.2d 391 (Md. Ct. Spec. App. 1988). A man forced a woman jogging in a park into the woods, where, as the court of appeals put it, he "rav ...
... 7. See David H. Kaye, The Admissibility ofDNA Testing, 13 CARDOZO L. REV. 353, 357 n.17 (1991). A case that is representative of this epoch is Cobey v. State, 559 A.2d 391 (Md. Ct. Spec. App. 1988). A man forced a woman jogging in a park into the woods, where, as the court of appeals put it, he "rav ...
- NRC Research Press
... blocks using a deletion mapping approach. All three chromosomes, 5A, 5B, and 5D, were shown to have a similar structure, apart from the 4A–5A translocation on the distal end of chromosome arm 5AL. The physical mapping of rice markers on the deletion lines revealed that the whole of rice chromosome 9 ...
... blocks using a deletion mapping approach. All three chromosomes, 5A, 5B, and 5D, were shown to have a similar structure, apart from the 4A–5A translocation on the distal end of chromosome arm 5AL. The physical mapping of rice markers on the deletion lines revealed that the whole of rice chromosome 9 ...
18. The Reference Sequence (RefSeq) Project
... The RefSeq record has been predicted by genome sequence analysis, but it is not yet supported by experimental evidence. The record may be partially supported by homology data. The RefSeq record has not yet been subject to individual review, and some aspect of the RefSeq record is predicted. The RefS ...
... The RefSeq record has been predicted by genome sequence analysis, but it is not yet supported by experimental evidence. The record may be partially supported by homology data. The RefSeq record has not yet been subject to individual review, and some aspect of the RefSeq record is predicted. The RefS ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... cover a whole set of sequences. Unlike HYDEN, each primer is checked so that it fits biological ...
... cover a whole set of sequences. Unlike HYDEN, each primer is checked so that it fits biological ...
Arsenite as an Electron Donor for Anoxygenic Photosynthesis
... gene: 25 µL 2× Taq mixture (Promega), 5 µL 10× reaction buffer, and 5 µL each of 2 µM forward and reverse primer (8F, 5’-GAGTTTGATCCTGGCTCAG-3’ and 1492R, 5’-GGTTACCTTGTTACGACTT-3’). The PCR products were cloned using the Invitrogen TA-TOPO cloning kit according to the manufacturer’s instructions. C ...
... gene: 25 µL 2× Taq mixture (Promega), 5 µL 10× reaction buffer, and 5 µL each of 2 µM forward and reverse primer (8F, 5’-GAGTTTGATCCTGGCTCAG-3’ and 1492R, 5’-GGTTACCTTGTTACGACTT-3’). The PCR products were cloned using the Invitrogen TA-TOPO cloning kit according to the manufacturer’s instructions. C ...
pdf
... DNA was quantified using PicoGreen (Molecular Probes), and quantitative PCR (QPCR) analyses were performed on an Mx3000p real-time PCR system (Stratagene) using a QuantiTect SYBR Green PCR master mix kit (Qiagen). Three gene fragments were quantified: total nirS (Tot nirS), a nirS gene sequence that ...
... DNA was quantified using PicoGreen (Molecular Probes), and quantitative PCR (QPCR) analyses were performed on an Mx3000p real-time PCR system (Stratagene) using a QuantiTect SYBR Green PCR master mix kit (Qiagen). Three gene fragments were quantified: total nirS (Tot nirS), a nirS gene sequence that ...
Journal of Bacteriology
... which contains a promoterless lacZ gene downstream of a multicloning site, was used to create a dnaK::lacZ transcriptional fusion. The region upstream of dnaK was amplified from PCL1445 by PCR using primer oMP870 (5⬘ TCAAGCGCT ACAACCTCGAGG 3⬘) and primer oMP871 (5⬘ GCATGCCATGTTAACTCT CCCGAAAC 3⬘) in ...
... which contains a promoterless lacZ gene downstream of a multicloning site, was used to create a dnaK::lacZ transcriptional fusion. The region upstream of dnaK was amplified from PCL1445 by PCR using primer oMP870 (5⬘ TCAAGCGCT ACAACCTCGAGG 3⬘) and primer oMP871 (5⬘ GCATGCCATGTTAACTCT CCCGAAAC 3⬘) in ...
Prediction and investigation of novel proteins in DNA double
... interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on their PPI profiles. In this study we aim to screen for novel proteins involved in NHEJ using PPI predictions. We use a computational tool to predict novel PPIs betwee ...
... interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on their PPI profiles. In this study we aim to screen for novel proteins involved in NHEJ using PPI predictions. We use a computational tool to predict novel PPIs betwee ...
BLAST - UCSD CSE
... • 1988: SBH suggested as an an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. • 1994: Affymetrix develops first 64-kb DNA microarray ...
... • 1988: SBH suggested as an an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. • 1994: Affymetrix develops first 64-kb DNA microarray ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.