Extensive tRNA gene changes in synthetic Brassica
... codon of mRNA. tRNA molecules with specific anticodons are known as isoacceptors, and read different codons to produce the same amino acid. There are 21 isoacceptor families, 20 for standard amino acids and one for selenocysteine (Goodenbour and Pan 2006). The secondary structure of tRNA consists of ...
... codon of mRNA. tRNA molecules with specific anticodons are known as isoacceptors, and read different codons to produce the same amino acid. There are 21 isoacceptor families, 20 for standard amino acids and one for selenocysteine (Goodenbour and Pan 2006). The secondary structure of tRNA consists of ...
Genomic overview of serine proteases
... functional domains for each enzyme are shown. As previously reported, phylogenetic trees appear to be governed by surface-exposed residues that control substrate and modulatory ligand recognition [17]. The tree presented here agrees with a previous attempt to segregate proteases into functional grou ...
... functional domains for each enzyme are shown. As previously reported, phylogenetic trees appear to be governed by surface-exposed residues that control substrate and modulatory ligand recognition [17]. The tree presented here agrees with a previous attempt to segregate proteases into functional grou ...
Alternative Splicing in Higher Plants
... For these alternative splicing isoforms that have a frameshift: - In Arabidopsis, 42% of these frameshifted events are found to introduce a premature stop codon >50bp upstream of the last exon-exon junction and are NMD candidates -In rice, this percentage is 36% This level of producing NMD candidate ...
... For these alternative splicing isoforms that have a frameshift: - In Arabidopsis, 42% of these frameshifted events are found to introduce a premature stop codon >50bp upstream of the last exon-exon junction and are NMD candidates -In rice, this percentage is 36% This level of producing NMD candidate ...
Modeling Trabecular Microstructure Evolution via Genetic Algorithm by Samuel W. L. Shames
... between 0 and 1, are used to determine a probability distribution when randomly selecting pixels to serve as vertices for struts. A pixel with an intensity closer to 1 is more likely to be selected, resulting a random weighted pixel distribution function. . . . . . . . . . . . . . . . . . . . . . . ...
... between 0 and 1, are used to determine a probability distribution when randomly selecting pixels to serve as vertices for struts. A pixel with an intensity closer to 1 is more likely to be selected, resulting a random weighted pixel distribution function. . . . . . . . . . . . . . . . . . . . . . . ...
A Physical Gene Map of the Bacteriophage P22 Late
... cleavage with EcoRI, BamHI, or EcoRI plus BamHI were cloned in Escherichia coli using the plasmid vector pBR322, and the resulting recombinant plasmids were introduced into Salmonella typhimurium. The genes present on a cloned fragment were identified by the ability of the hybrid plasmid to compleme ...
... cleavage with EcoRI, BamHI, or EcoRI plus BamHI were cloned in Escherichia coli using the plasmid vector pBR322, and the resulting recombinant plasmids were introduced into Salmonella typhimurium. The genes present on a cloned fragment were identified by the ability of the hybrid plasmid to compleme ...
Using Bioconductor`s Annotation Libraries
... libraries. Platform specific libraries are a group annotation libraries assembled specifically for given platforms (e. g. Affymetrix HG U95Av2). org.XX.eg.db are libraries containing data assembled at genome level for specific organisms such as human, mouse, fly, or rat. KEGG.db and GO.db are source ...
... libraries. Platform specific libraries are a group annotation libraries assembled specifically for given platforms (e. g. Affymetrix HG U95Av2). org.XX.eg.db are libraries containing data assembled at genome level for specific organisms such as human, mouse, fly, or rat. KEGG.db and GO.db are source ...
Supplementary Figure Legends (doc 34K)
... complete homozygosity situation in only 10% of the cases (step ). Around half of them harbour a complete or partial deletion of the NF1 locus as confirmed by real-time PCR-based gene dosage. Custom high resolution array-CGH enables the accurate characterization of the deletion type. NF1 complete an ...
... complete homozygosity situation in only 10% of the cases (step ). Around half of them harbour a complete or partial deletion of the NF1 locus as confirmed by real-time PCR-based gene dosage. Custom high resolution array-CGH enables the accurate characterization of the deletion type. NF1 complete an ...
Divergent Evolutionary and Expression Patterns between Lineage
... syntenic regions between A. thaliana and the rest of three species A. lyrata, C. rubella, B. rapa; second, the gene did not have any reciprocal ortholog in A. lyrata, C. rubella and B. rapa. Using the pipelines developed by UCSC genome browser [54], we constructed the reciprocal syntenic relationshi ...
... syntenic regions between A. thaliana and the rest of three species A. lyrata, C. rubella, B. rapa; second, the gene did not have any reciprocal ortholog in A. lyrata, C. rubella and B. rapa. Using the pipelines developed by UCSC genome browser [54], we constructed the reciprocal syntenic relationshi ...
Article Old but Not (So) Degenerated—Slow
... between group 1 and group 2 (P = 0.006, Tukey’s post-hoc test) and between group 1 and group 3 (P = 0.0005). Although these three regions could potentially be seen to represent evolutionary strata, we cannot draw definite conclusions on this point because the order and character of inversion events ...
... between group 1 and group 2 (P = 0.006, Tukey’s post-hoc test) and between group 1 and group 3 (P = 0.0005). Although these three regions could potentially be seen to represent evolutionary strata, we cannot draw definite conclusions on this point because the order and character of inversion events ...
PCR Clean-up Kit / 96-well PCR Clean
... 4. Removal of impurities by filtration and selective binding of DNA The insoluble debris complex, consisting of bacterial genomic DNA, bacterial cell wall debris and SDS/salt is removed by filtration through the Filtration Plate. This plate contains specific porous materials which selectively remove ...
... 4. Removal of impurities by filtration and selective binding of DNA The insoluble debris complex, consisting of bacterial genomic DNA, bacterial cell wall debris and SDS/salt is removed by filtration through the Filtration Plate. This plate contains specific porous materials which selectively remove ...
Analyses of 16S rRNA and RuBisCO large subunit genes from an
... Amplification and Cloning of 16S rRNA and RuBisCO genes The 16S rDNA was amplified from bulk extracted DNA using well-known primers Eubac27F and Eubac1492R (DeLong, 1992). The primer sets for the amplification of RuBisCO Form I and Form II genes (cbbL and cbbM) were designed and used previously by E ...
... Amplification and Cloning of 16S rRNA and RuBisCO genes The 16S rDNA was amplified from bulk extracted DNA using well-known primers Eubac27F and Eubac1492R (DeLong, 1992). The primer sets for the amplification of RuBisCO Form I and Form II genes (cbbL and cbbM) were designed and used previously by E ...
Power Point
... independently. However, advances in genetics have shown that an abnormal situation, called crossing-over, can occur between genes for different traits. ...
... independently. However, advances in genetics have shown that an abnormal situation, called crossing-over, can occur between genes for different traits. ...
animal genetics
... independently. However, advances in genetics have shown that an abnormal situation, called crossing-over, can occur between genes for different traits. ...
... independently. However, advances in genetics have shown that an abnormal situation, called crossing-over, can occur between genes for different traits. ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
Explaining the Likelihood Ratio in DNA Mixture
... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
Characteristics, causes and evolutionary consequences of male
... genomes. It might also be argued that focusing on the same sequence inserted at many different locations in the genome reduces the confounding effects of mutation rate heterogeneity related to inherent features of the sequence context, like nucleotide composition. However, interspersed elements tend ...
... genomes. It might also be argued that focusing on the same sequence inserted at many different locations in the genome reduces the confounding effects of mutation rate heterogeneity related to inherent features of the sequence context, like nucleotide composition. However, interspersed elements tend ...
Genomic organisation of the Mal d 1 gene cluster on linkage group
... PCR-based screening of the BAC library A BAC library from the cultivar Florina (Vinatzer et al. 1998) already available at the Department of Fruit Tree and Woody Plant Sciences (University of Bologna) was used. The library consists of 36,864 BAC clones with an average insert size of 120 kb, represen ...
... PCR-based screening of the BAC library A BAC library from the cultivar Florina (Vinatzer et al. 1998) already available at the Department of Fruit Tree and Woody Plant Sciences (University of Bologna) was used. The library consists of 36,864 BAC clones with an average insert size of 120 kb, represen ...
Evolution of a pseudo-control region in the mitochondrial genome of
... Sequences of the mitochondrial genome were employed to study the phylogenetic relationships of the Palearctic buzzards (genus Buteo).In a first approach a 2.5 kb PCR fragment comprising the section between ND6 and 12s genes was isolated from Buteo buteo hureo,cloned and sequenced. The gene order cor ...
... Sequences of the mitochondrial genome were employed to study the phylogenetic relationships of the Palearctic buzzards (genus Buteo).In a first approach a 2.5 kb PCR fragment comprising the section between ND6 and 12s genes was isolated from Buteo buteo hureo,cloned and sequenced. The gene order cor ...
Chapter 14
... helix makes contacts with particular bases in the operator sequence that it recognizes. ...
... helix makes contacts with particular bases in the operator sequence that it recognizes. ...
Sequence Alignment - Bilkent University
... Schematic representation of the multiple alignment of the mRNAs of a microsomal glutathione transferase homolog gene with the genomic sequence. Three GenBank mRNAs (blue) align to the same genomic locus on chromosome 9, NT_008541 (red). Three ESTs that map to this locus are presented (purple), 38 ot ...
... Schematic representation of the multiple alignment of the mRNAs of a microsomal glutathione transferase homolog gene with the genomic sequence. Three GenBank mRNAs (blue) align to the same genomic locus on chromosome 9, NT_008541 (red). Three ESTs that map to this locus are presented (purple), 38 ot ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... Mitochondria within human cells contain vast numbers of mitochondrial DNA (mtDNA), which are small, circular, and double-stranded. The proper functions of mtDNA depend totally on specific proteins that are encoded by the nucleus and then imported into mitochondria. Thus instability of mtDNA can stem ...
... Mitochondria within human cells contain vast numbers of mitochondrial DNA (mtDNA), which are small, circular, and double-stranded. The proper functions of mtDNA depend totally on specific proteins that are encoded by the nucleus and then imported into mitochondria. Thus instability of mtDNA can stem ...
Development and application of a positive
... implied through gene disruption alone. Such reversible recombination can therefore be employed for both the rapid analysis of the phenotype by targeted disruption of a gene and further associate phenotype and function by genotype restoration through the use of a single plasmid and a single positive ...
... implied through gene disruption alone. Such reversible recombination can therefore be employed for both the rapid analysis of the phenotype by targeted disruption of a gene and further associate phenotype and function by genotype restoration through the use of a single plasmid and a single positive ...
GENtle, a free multi-purpose molecular biology tool
... but because of the abundance thereof. This so-called “information overload” not only affects publications, with PubMed entries currently increasing at ca. 3000 articles per day, but also raw data repositories. The number of known DNA sequences and genomes increases at an exponential rate (Figure 1). ...
... but because of the abundance thereof. This so-called “information overload” not only affects publications, with PubMed entries currently increasing at ca. 3000 articles per day, but also raw data repositories. The number of known DNA sequences and genomes increases at an exponential rate (Figure 1). ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.