
Maternal uniparental disomyof chromosome 13
... reported involving all or part of chromosomes homozygous despite all being highly (>75%) 6,7,11,14,15,16,21,22, and the XY pair.'4 polymorphic.'2 Such a chromosome could have These investigations are of value firstly for been formed by a translocation involving the determining which chromosomes cont ...
... reported involving all or part of chromosomes homozygous despite all being highly (>75%) 6,7,11,14,15,16,21,22, and the XY pair.'4 polymorphic.'2 Such a chromosome could have These investigations are of value firstly for been formed by a translocation involving the determining which chromosomes cont ...
the x chromosome in population genetics
... The lower mutation rate and the smaller population size of the X chromosome, compared with autosomes, lead to an unambiguous prediction that genetic diversity should also be lower there.As measurements of the diversity at individual loci on the X chromosome and on autosomes vary widely (as discussed ...
... The lower mutation rate and the smaller population size of the X chromosome, compared with autosomes, lead to an unambiguous prediction that genetic diversity should also be lower there.As measurements of the diversity at individual loci on the X chromosome and on autosomes vary widely (as discussed ...
YEAST GENETICS AND MOLECULAR BIOLOGY
... Genetic markers are used to follow chromosomes in genetic crosses, to select diploids in genetic crosses, to select transformants in transformation with plasmids or integration of genes into the genome Commonly genetic markers cause auxotrophies: HIS3, URA3, TRP1, LEU2, LYS2, ADE2 The ade2 mutation ...
... Genetic markers are used to follow chromosomes in genetic crosses, to select diploids in genetic crosses, to select transformants in transformation with plasmids or integration of genes into the genome Commonly genetic markers cause auxotrophies: HIS3, URA3, TRP1, LEU2, LYS2, ADE2 The ade2 mutation ...
Adherin - Semantic Scholar
... mutations in the budding yeast Saccharomyces cerevisiae disrupt mitotic chromatid cohesion [3]. A number of new papers report evidence that the chromosome cohesion function of adherin is conserved in higher organisms. Rollins et al. [4] found Drosophila Nipped-B adherin mutations, which reduce trans ...
... mutations in the budding yeast Saccharomyces cerevisiae disrupt mitotic chromatid cohesion [3]. A number of new papers report evidence that the chromosome cohesion function of adherin is conserved in higher organisms. Rollins et al. [4] found Drosophila Nipped-B adherin mutations, which reduce trans ...
Article The Pseudoautosomal Regions of the U/V Sex
... size (Spearman’s correlation test = 0.113, P = 0.598) whereas gene density and GC percentage increase with ...
... size (Spearman’s correlation test = 0.113, P = 0.598) whereas gene density and GC percentage increase with ...
transposon
... present in reverse orientation at the ends of some transposons. direct repeats : identical (or closely related) sequences present in two or more copies in the same orientation; they are not necessarily adjacent. transposase : the enzyme involved in insertion of transposon at a new site. ...
... present in reverse orientation at the ends of some transposons. direct repeats : identical (or closely related) sequences present in two or more copies in the same orientation; they are not necessarily adjacent. transposase : the enzyme involved in insertion of transposon at a new site. ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
Manipulating the Plasmodium Genome
... a basic surveillance system that degrades transposon or viral messages, has already been harnessed as a reverse genetics tool. Injecting or expressing double-stranded RNAs causes the silencing of the corresponding gene in many systems tested, from protozoa to multicellular organisms. Degradation of ...
... a basic surveillance system that degrades transposon or viral messages, has already been harnessed as a reverse genetics tool. Injecting or expressing double-stranded RNAs causes the silencing of the corresponding gene in many systems tested, from protozoa to multicellular organisms. Degradation of ...
Clustering of mandibular organ-inhibiting hormone and moult
... were placed in group 3. Three of them only contained the MO-IH-2 gene and were placed in group 4. The overall maps for each of the groups are given in Fig. 2, from which it can be seen that groups 1–3 are overlapping examples of a single type of gene organisation but with a polymorphic TaqI site. ...
... were placed in group 3. Three of them only contained the MO-IH-2 gene and were placed in group 4. The overall maps for each of the groups are given in Fig. 2, from which it can be seen that groups 1–3 are overlapping examples of a single type of gene organisation but with a polymorphic TaqI site. ...
Nucleosomal structure of sea urchin and starfish sperm chromatin
... starfish sperm H2B. The amino acid composition of sea urchin sperm H2B is characterized by a very high arginine content. The primary sequence analysis of six H2B subtractions from sperm of three sea urchin species has shown that the increase in mol. w. is due to the elongation of the N-terminal regi ...
... starfish sperm H2B. The amino acid composition of sea urchin sperm H2B is characterized by a very high arginine content. The primary sequence analysis of six H2B subtractions from sperm of three sea urchin species has shown that the increase in mol. w. is due to the elongation of the N-terminal regi ...
Mendelian Genetics
... oSo if diseases are caused by small mutations they cannot be predicted •Individual DNA strands or genes •The number of genes in any given area of a chromosome ...
... oSo if diseases are caused by small mutations they cannot be predicted •Individual DNA strands or genes •The number of genes in any given area of a chromosome ...
3-A Notes
... oSo if diseases are caused by small mutations they cannot be predicted •Individual DNA strands or genes •The number of genes in any given area of a chromosome ...
... oSo if diseases are caused by small mutations they cannot be predicted •Individual DNA strands or genes •The number of genes in any given area of a chromosome ...
Factors Affecting synonymous codon Usage Bias in chloroplast
... of the chloroplast genome is 37.0%. Coding regions make up 49.94% of the chloroplast genome (41.86% protein-coding genes, 8.08% RNA genes) and noncoding regions, which contain intergenic spacer (IGS) regions and introns, comprising 50.06%. Among the full 128 coding genes of the Oncidium Gower Rams ...
... of the chloroplast genome is 37.0%. Coding regions make up 49.94% of the chloroplast genome (41.86% protein-coding genes, 8.08% RNA genes) and noncoding regions, which contain intergenic spacer (IGS) regions and introns, comprising 50.06%. Among the full 128 coding genes of the Oncidium Gower Rams ...
Molecular Diagnostics for the Detection and Characterization of
... The technical feasibility of these assays has already been demonstrated. How these new, often more expensive tests will be incorporated into routine practice and the impact they will have on patient care remain to be determined. One of the most attractive uses for such techniques is to achieve a mor ...
... The technical feasibility of these assays has already been demonstrated. How these new, often more expensive tests will be incorporated into routine practice and the impact they will have on patient care remain to be determined. One of the most attractive uses for such techniques is to achieve a mor ...
NUCLEOTIDES AND NUCLEIC ACIDS
... thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broader range of functions, and several classes are found in cells. Ribosomal RNAs (rRNAs) are components of ribosom ...
... thousands of genes, and DNA molecules, not surprisingly, tend to be very large. The storage and transmission of biological information are the only known functions of DNA. RNAs have a broader range of functions, and several classes are found in cells. Ribosomal RNAs (rRNAs) are components of ribosom ...
Using an Alu Insertion Polymorphism to Study Human
... identity testing. Many polymorphisms are located in the estimated 98% of the human genome that does not encode protein. This experiment examines a polymorphism in the human genome that is caused by the insertion of an Alu transposon, or transposable element. Alu is a member of the family of short in ...
... identity testing. Many polymorphisms are located in the estimated 98% of the human genome that does not encode protein. This experiment examines a polymorphism in the human genome that is caused by the insertion of an Alu transposon, or transposable element. Alu is a member of the family of short in ...
Protein export elements from Lactococcus lactis
... bacteria has scarcely been studied, these bacteria are known to export several proteins, some of which are secretedinto the growth medium. Thus, lactococci export a cell wall-associatedserine proteinase essentialfor rapid growth in milk (Kok and Venerna 1988),and they secrete Usp45, a protein of unk ...
... bacteria has scarcely been studied, these bacteria are known to export several proteins, some of which are secretedinto the growth medium. Thus, lactococci export a cell wall-associatedserine proteinase essentialfor rapid growth in milk (Kok and Venerna 1988),and they secrete Usp45, a protein of unk ...
PowerPoint 프레젠테이션
... siRNA Target Site Selection We have developed some software tools to facilitate your design process. It is recommended that at least 3 vector-based siRNA should be prepared for each gene in order to find a potent and specific siRNA. Here are the reasons: 1.Not all siRNA target sequences are equally ...
... siRNA Target Site Selection We have developed some software tools to facilitate your design process. It is recommended that at least 3 vector-based siRNA should be prepared for each gene in order to find a potent and specific siRNA. Here are the reasons: 1.Not all siRNA target sequences are equally ...
Identification of sixteen single-nucleotide polymorphism markers in
... salar (Hayes et al. 2007), Crassostrea virginica (Zhang and Guo 2010) and Chlamys farreri (Li et al. 2013). However, to our knowledge, although the draft genome of P. fucata has been established which could provide a platform for the identification of selection markers (Takeuchi et al. 2012), there ...
... salar (Hayes et al. 2007), Crassostrea virginica (Zhang and Guo 2010) and Chlamys farreri (Li et al. 2013). However, to our knowledge, although the draft genome of P. fucata has been established which could provide a platform for the identification of selection markers (Takeuchi et al. 2012), there ...
Expression of phosphofructokinase in Neisseria meningitidis
... recombination, these homologous sequences must also contain a DNA uptake sequence. A kanamycin resistance gene must be present between the homologous regions to select for transformants after transformation. The final plasmid constructed in this study that was used for transformation of N. meningiti ...
... recombination, these homologous sequences must also contain a DNA uptake sequence. A kanamycin resistance gene must be present between the homologous regions to select for transformants after transformation. The final plasmid constructed in this study that was used for transformation of N. meningiti ...
BIO301 - National Open University of Nigeria
... same species. Examples of gene flow within a species include the migration and then breeding of organisms, or the exchange of pollen. Gene transfer between species includes the formation of hybrid organisms and horizontal gene transfer. Migration into or out of a population can change allele frequen ...
... same species. Examples of gene flow within a species include the migration and then breeding of organisms, or the exchange of pollen. Gene transfer between species includes the formation of hybrid organisms and horizontal gene transfer. Migration into or out of a population can change allele frequen ...
Genomic library

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.