K - Romanian Biotechnological Letters
... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
Genes Practice Questions
... 10 A landmark study in DNA replication research by Meselson and Stahl involved growing bacteria including an isotope of nitrogen 15N and then placing these bacteria in a medium containing only 14N. According to the known method of DNA replication, what do you predict the ratio of the two isotopes wo ...
... 10 A landmark study in DNA replication research by Meselson and Stahl involved growing bacteria including an isotope of nitrogen 15N and then placing these bacteria in a medium containing only 14N. According to the known method of DNA replication, what do you predict the ratio of the two isotopes wo ...
Vibrio diabolicus sp. nov., a New Polysaccharide
... RNase (5 pg/ml) at 60°C for 1 h. This step was followed by one additional extraction with phenol-chloroform-isoamyl alcohol (24:24:1) and one extraction with chloroform. The DNA was precipitated by adding 2.5 volumes of cold pure ethanol, and the pellet was dried and suspended in TE buffer (10 mM Tr ...
... RNase (5 pg/ml) at 60°C for 1 h. This step was followed by one additional extraction with phenol-chloroform-isoamyl alcohol (24:24:1) and one extraction with chloroform. The DNA was precipitated by adding 2.5 volumes of cold pure ethanol, and the pellet was dried and suspended in TE buffer (10 mM Tr ...
pdf
... measure of the genetic distance between them (reviewed in Chapter 1). The recombinant chromosomes resulting from a crossover are revealed in a mating between the heterozygous parent (A+B+/A-B-) and a homozygous recessive individual (A-B-/A-B-). Most of the germ cells contributed by the heterozygous ...
... measure of the genetic distance between them (reviewed in Chapter 1). The recombinant chromosomes resulting from a crossover are revealed in a mating between the heterozygous parent (A+B+/A-B-) and a homozygous recessive individual (A-B-/A-B-). Most of the germ cells contributed by the heterozygous ...
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr
... ombination function an nd enzymess of phage λ (17, 18). f constructing chrom mosomally engineered d λ‐red recombineeringg is a highlly effectivee method for Escherichia a coli strainss. This techn nique is far more conveenient than n restriction n enzyme syystems sincee it can man n ...
... ombination function an nd enzymess of phage λ (17, 18). f constructing chrom mosomally engineered d λ‐red recombineeringg is a highlly effectivee method for Escherichia a coli strainss. This techn nique is far more conveenient than n restriction n enzyme syystems sincee it can man n ...
PhoB by Phosphate Stress and Controlled by
... searches of major databases (52). To more extensively characterize this locus, we used a fragment of DNA flanking the transposon to probe an S. meliloti cosmid library (2) and obtained cosmid p7C9, which contained ackA. Restriction mapping, Southern blot analysis, and enzyme assays following Pi star ...
... searches of major databases (52). To more extensively characterize this locus, we used a fragment of DNA flanking the transposon to probe an S. meliloti cosmid library (2) and obtained cosmid p7C9, which contained ackA. Restriction mapping, Southern blot analysis, and enzyme assays following Pi star ...
Genome duplications and accelerated evolution of
... be established, but it does not allow for precise identification of their cluster affiliation, due to the limited number of variable amino acid positions in the homeobox. Furthermore, gene absence cannot be unambiguously confirmed due to the inherent problems of PCR template competition when amplify ...
... be established, but it does not allow for precise identification of their cluster affiliation, due to the limited number of variable amino acid positions in the homeobox. Furthermore, gene absence cannot be unambiguously confirmed due to the inherent problems of PCR template competition when amplify ...
Applied Microbiology and Biotechnology
... To identify DB3, the 5′- and 3′-ends of a 1.5 kb PCR product of the 16S rRNA gene were sequenced. The sequence shares strong identities with 16S rRNA genes from various Ralstonia strains (Table 1), especially with strain PHS 1 (Lee and Lee 2000). For DB4, the sequence of a 16S rRNA gene specific seg ...
... To identify DB3, the 5′- and 3′-ends of a 1.5 kb PCR product of the 16S rRNA gene were sequenced. The sequence shares strong identities with 16S rRNA genes from various Ralstonia strains (Table 1), especially with strain PHS 1 (Lee and Lee 2000). For DB4, the sequence of a 16S rRNA gene specific seg ...
- Genetics
... In the accompanying article in this issue (Bergero et al. 2013), we mapped several genes to the S. latifolia PAR. The 25-cM genetic map length of the S. latifolia PAR suggests that it probably carries many genes that could potentially undergo SA mutations, and so this species is well suited for tes ...
... In the accompanying article in this issue (Bergero et al. 2013), we mapped several genes to the S. latifolia PAR. The 25-cM genetic map length of the S. latifolia PAR suggests that it probably carries many genes that could potentially undergo SA mutations, and so this species is well suited for tes ...
light - Microbiology
... (1948). Following the discovery of sexual differentiation, Hayes (1952) found that the effect is exclusively due to an increase in the fertility of the donor (F+ male) population ; irradiation of the recipient (F- female) parental bacteria decreases the number of recombinants in proportion to the su ...
... (1948). Following the discovery of sexual differentiation, Hayes (1952) found that the effect is exclusively due to an increase in the fertility of the donor (F+ male) population ; irradiation of the recipient (F- female) parental bacteria decreases the number of recombinants in proportion to the su ...
functional analysis of chromatin assembly genes in tetrahymena
... Amplification and molecular cloning of ~1Kb homologous sequences flanking up and ...
... Amplification and molecular cloning of ~1Kb homologous sequences flanking up and ...
The f ructokinase f rom Rhizobium leguminosarum
... since frk mutants have been shown to lack capsule production when grown on medium containing mannitol (McLaughlin & Hughes, 1989). Kanamycin (150 pg ml-l) was used as a counter-selection agent against donor strains. Three clones conferring the mucoid phenotype (pB12, pB30 and pB40) contained the pla ...
... since frk mutants have been shown to lack capsule production when grown on medium containing mannitol (McLaughlin & Hughes, 1989). Kanamycin (150 pg ml-l) was used as a counter-selection agent against donor strains. Three clones conferring the mucoid phenotype (pB12, pB30 and pB40) contained the pla ...
Ultraviolet Induction of Chromosome Transfer by
... (1948). Following the discovery of sexual differentiation, Hayes (1952) found that the effect is exclusively due to an increase in the fertility of the donor (F+ male) population ; irradiation of the recipient (F- female) parental bacteria decreases the number of recombinants in proportion to the su ...
... (1948). Following the discovery of sexual differentiation, Hayes (1952) found that the effect is exclusively due to an increase in the fertility of the donor (F+ male) population ; irradiation of the recipient (F- female) parental bacteria decreases the number of recombinants in proportion to the su ...
SNP discovery
... all’Adige, Trento, Italy, 4 Laboratory for Fruit Breeding and Biotechnology, Department of Biosystems, Katholieke Universiteit Leuven, Heverlee, Leuven, Belgium, 5 USDA-ARS, National Clonal Germplasm Repository, Corvallis, Oregon, United States of America, 6 Illumina Inc., Hayward, California, Unite ...
... all’Adige, Trento, Italy, 4 Laboratory for Fruit Breeding and Biotechnology, Department of Biosystems, Katholieke Universiteit Leuven, Heverlee, Leuven, Belgium, 5 USDA-ARS, National Clonal Germplasm Repository, Corvallis, Oregon, United States of America, 6 Illumina Inc., Hayward, California, Unite ...
View PDF - Genetics
... selected Arabidopsis thaliana amino acid sequences as queries against each of the assembled Sileneae transcriptomes. The top hit in each transcriptome was retrieved with a custom Perl script using BioPerl modules (Stajich et al. 2002). In cases where there were multiple paralogous genes, manual cu ...
... selected Arabidopsis thaliana amino acid sequences as queries against each of the assembled Sileneae transcriptomes. The top hit in each transcriptome was retrieved with a custom Perl script using BioPerl modules (Stajich et al. 2002). In cases where there were multiple paralogous genes, manual cu ...
Defining the Epigenetic Mechanism of Asymmetric Cell Division of
... named) Watson strand-containing chromatid is switching competent, and its sister cell, inheriting the template Crick strand-containing chromatid, is always incompetent (Klar 1987a, 1990, 2007). The difference in strands is the result of the DNA sequence- and strand-specific imprint moiety installed a ...
... named) Watson strand-containing chromatid is switching competent, and its sister cell, inheriting the template Crick strand-containing chromatid, is always incompetent (Klar 1987a, 1990, 2007). The difference in strands is the result of the DNA sequence- and strand-specific imprint moiety installed a ...
Part III: Laboratory – Electrophoresis
... four methods (DNA extraction, Polymerase Chain Reaction (PCR), and gel. electrophoresis) that are commonly used in biological research. about DNA polymorphisms and how they are used in mapping genes. about Arabidopsis thaliana, an important research model for molecular genetics. about develo ...
... four methods (DNA extraction, Polymerase Chain Reaction (PCR), and gel. electrophoresis) that are commonly used in biological research. about DNA polymorphisms and how they are used in mapping genes. about Arabidopsis thaliana, an important research model for molecular genetics. about develo ...
Insights into three whole-genome duplications gleaned
... remains (Figure 2). We were able to align 5,781 P. caudatum genes with all 12 of their syntenic aurelia orthologs (when present), which includes 10,907 P. biaurelia, 10,970 P. tetraurelia, and 10,024 P. sexaurelia genes (Files S1-S2). This represents about 30% of the genes in each of the aligned gen ...
... remains (Figure 2). We were able to align 5,781 P. caudatum genes with all 12 of their syntenic aurelia orthologs (when present), which includes 10,907 P. biaurelia, 10,970 P. tetraurelia, and 10,024 P. sexaurelia genes (Files S1-S2). This represents about 30% of the genes in each of the aligned gen ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... of cells and nucleic acids prior to DNA purification. DDH was carried out as described by De Ley et al. (1970) under consideration of the modifications described by Huss et al. (1983) using a model Cary 100 Bio UV/VIS-spectrophotometer with a Peltier-thermostat-equipped 666 multicell changer and a t ...
... of cells and nucleic acids prior to DNA purification. DDH was carried out as described by De Ley et al. (1970) under consideration of the modifications described by Huss et al. (1983) using a model Cary 100 Bio UV/VIS-spectrophotometer with a Peltier-thermostat-equipped 666 multicell changer and a t ...
PTC PCR II Manual
... a haplotype, correlates most strongly with tasting ability. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the ...
... a haplotype, correlates most strongly with tasting ability. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the ...
Plants` Epigenetic Secrets
... There are three different types of DNA methylation in plants: CG, CHH (where H is any base except G), and CHG. In Arabidopsis, CG methylation is found on some genes, but primarily on repeat sequences that make up transposons, as well as other repeat sequences in the genome. CHH methylation is found ...
... There are three different types of DNA methylation in plants: CG, CHH (where H is any base except G), and CHG. In Arabidopsis, CG methylation is found on some genes, but primarily on repeat sequences that make up transposons, as well as other repeat sequences in the genome. CHH methylation is found ...
Widespread expression of the bovine Agouti gene results from at
... cDNA revealed three transcript isoforms. An mRNA of approximately 800 nucleotides, corresponding to the full-length cDNA (732 bp) obtained by the RACE-PCR experiment, was detected in skin. Brain, spleen, lung, liver and kidney mainly express a 2 kb mRNA isoform. A 1.5 kb mRNA isoform is predominant ...
... cDNA revealed three transcript isoforms. An mRNA of approximately 800 nucleotides, corresponding to the full-length cDNA (732 bp) obtained by the RACE-PCR experiment, was detected in skin. Brain, spleen, lung, liver and kidney mainly express a 2 kb mRNA isoform. A 1.5 kb mRNA isoform is predominant ...
BLSSpeller: exhaustive comparative discovery of
... collection of binding sites. The algorithm can be run in both alignment-free or alignment-based mode. In case of alignment-free discovery, the conservation of a motif is scored irrespective of its orientation or position within a promoter sequence. This relaxed definition of conservation was previou ...
... collection of binding sites. The algorithm can be run in both alignment-free or alignment-based mode. In case of alignment-free discovery, the conservation of a motif is scored irrespective of its orientation or position within a promoter sequence. This relaxed definition of conservation was previou ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.