Bioinformatics with basic local alignment search tool (BLAST) and
... such as Protein Data Bank. Secondly, bioinformatics seeks to develop tools for analysis of data, and thirdly, to use these tools to analyze and interpret data results, which is the focus of the current review. In recent times, application of computational approaches to biological data has become a v ...
... such as Protein Data Bank. Secondly, bioinformatics seeks to develop tools for analysis of data, and thirdly, to use these tools to analyze and interpret data results, which is the focus of the current review. In recent times, application of computational approaches to biological data has become a v ...
Complete
... It has been proposed that an array of spatially asymmetric obstacles could operate as Brownian ratchets, structures that permit Brownian motion in only one direction [1–7]. When particles flow through such an array driven by an electric field (Fig. 2.1A), particles diffusing to the left (path 1; Fig ...
... It has been proposed that an array of spatially asymmetric obstacles could operate as Brownian ratchets, structures that permit Brownian motion in only one direction [1–7]. When particles flow through such an array driven by an electric field (Fig. 2.1A), particles diffusing to the left (path 1; Fig ...
SEARCH_16S: A new algorithm for identifying 16S
... genes from eukaryotic cells which are classified as Proteobacteria by SILVA, and five were chloroplasts. The remaining six were all uncultured sequences. In the prokaryotic subset of RDP, only 84% of sequences were hits. This is due to the many partial genes in RDP which are too short to be reported ...
... genes from eukaryotic cells which are classified as Proteobacteria by SILVA, and five were chloroplasts. The remaining six were all uncultured sequences. In the prokaryotic subset of RDP, only 84% of sequences were hits. This is due to the many partial genes in RDP which are too short to be reported ...
File
... • Genome lies within nucleus of cell Comprised of 22 pairs of chromosomes During mitosis all pairs are copied During meiosis only one member of each pair are copied Karyotype © 2007 Thomson - Wadsworth ...
... • Genome lies within nucleus of cell Comprised of 22 pairs of chromosomes During mitosis all pairs are copied During meiosis only one member of each pair are copied Karyotype © 2007 Thomson - Wadsworth ...
HapTree-X: An integrative Bayesian framework for haplotype
... In genomic read data, all r ∈ R are equally likely to be sampled from the maternal or paternal chromosomes. In RNA-seq data however, this may not always be the case. In this paper, we define the differential haplotypic expression (DHE) to represent the underlying expression bias between the maternal ...
... In genomic read data, all r ∈ R are equally likely to be sampled from the maternal or paternal chromosomes. In RNA-seq data however, this may not always be the case. In this paper, we define the differential haplotypic expression (DHE) to represent the underlying expression bias between the maternal ...
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE
... By 1950, scientists knew DNA was the genetic material, but they did not know the structure of DNA. In 1953, Watson and Crick built a model of DNA that was consistent with available evidence. They used X-ray photos of DNA taken by Franklin and Wilkins as part of their research. ...
... By 1950, scientists knew DNA was the genetic material, but they did not know the structure of DNA. In 1953, Watson and Crick built a model of DNA that was consistent with available evidence. They used X-ray photos of DNA taken by Franklin and Wilkins as part of their research. ...
Slides
... heterochromatin: dense, compact structure during interphase generally near the centromere and telomeres (chromosome ends) composed of long tracks of fairly short base pair repeats few genes compared to euchromatin euchromatin: less dense DNA that only becomes visible after condensing typically has g ...
... heterochromatin: dense, compact structure during interphase generally near the centromere and telomeres (chromosome ends) composed of long tracks of fairly short base pair repeats few genes compared to euchromatin euchromatin: less dense DNA that only becomes visible after condensing typically has g ...
Genome-Wide Dissection of Hybrid Sterility in
... Since the AFLP technique was described (Vos et al. 1995), several studies have successfully used it as a rapid blind method for isolating molecular markers to saturate genetic maps. Perhaps the most important advantage of AFLPs markers is that they combine accuracy and reproducibility with their cap ...
... Since the AFLP technique was described (Vos et al. 1995), several studies have successfully used it as a rapid blind method for isolating molecular markers to saturate genetic maps. Perhaps the most important advantage of AFLPs markers is that they combine accuracy and reproducibility with their cap ...
Export To Word
... common to almost all organisms. Evaluate the impact of biotechnology on the individual, society and the environment, including medical and ethical issues. Remarks/Examples: Annually assessed on Biology EOC. Discuss the technologies associated with forensic medicine and DNA identification, including ...
... common to almost all organisms. Evaluate the impact of biotechnology on the individual, society and the environment, including medical and ethical issues. Remarks/Examples: Annually assessed on Biology EOC. Discuss the technologies associated with forensic medicine and DNA identification, including ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... collected in Ria de Aveiro, to perform the microcosm experiment, were 7.5, 35 g·l–1 and 26°C, respectively. A preliminary experiment was performed to evaluate the influence of Av27 and TBT in the natural microbial community. Figure 3(A) shows that the samples containing TBT and inoculated with the r ...
... collected in Ria de Aveiro, to perform the microcosm experiment, were 7.5, 35 g·l–1 and 26°C, respectively. A preliminary experiment was performed to evaluate the influence of Av27 and TBT in the natural microbial community. Figure 3(A) shows that the samples containing TBT and inoculated with the r ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
... dishes were incubated at 30oC for one to three weeks. The separated colony was removed to PDA slant and incubated at 30oC until the rough and white light grey spores was appeared. The isolation process was repeated to obtain the pure isolate. Morphology, physiology and biochemical characteristics Af ...
... dishes were incubated at 30oC for one to three weeks. The separated colony was removed to PDA slant and incubated at 30oC until the rough and white light grey spores was appeared. The isolation process was repeated to obtain the pure isolate. Morphology, physiology and biochemical characteristics Af ...
The Plastidic Phosphoglucomutase from
... An Arabidopsis cDNA (AtPGMp) encoding the plastidic phosphoglucomutase (PGM) predicted a 623-amino acid protein with an N-terminal sequence typical of a plastid signal peptide. Expression of a recombinant protein in Escherichia coli confirmed its enzyme activity. The recombinant enzyme had an appare ...
... An Arabidopsis cDNA (AtPGMp) encoding the plastidic phosphoglucomutase (PGM) predicted a 623-amino acid protein with an N-terminal sequence typical of a plastid signal peptide. Expression of a recombinant protein in Escherichia coli confirmed its enzyme activity. The recombinant enzyme had an appare ...
What is Cytogenetics?
... specific DNA sequences on chromosomes. • FISH uses fluorescent probes which bind only to those parts of the chromosome with which they show a high degree of sequence similarity. • Generally between 200-1000 cells are counted. ...
... specific DNA sequences on chromosomes. • FISH uses fluorescent probes which bind only to those parts of the chromosome with which they show a high degree of sequence similarity. • Generally between 200-1000 cells are counted. ...
Biotechnology Timeline
... are produced by transferring genes from other animals into mice. The first patent for a genetically modified organism is granted — for bacteria that can break down crude oil. ...
... are produced by transferring genes from other animals into mice. The first patent for a genetically modified organism is granted — for bacteria that can break down crude oil. ...
RPG-Consent-aCGH NGS for aneuploidy_2015_Final
... embryo may be incorrectly identified as an abnormal embryo and therefore not replaced in the uterus, or that a chromosomally abnormal embryo is incorrectly identified as a normal embryo, and transferred into the uterus. This technique can only assess the sampled tissue. The remainder and undiagnosed ...
... embryo may be incorrectly identified as an abnormal embryo and therefore not replaced in the uterus, or that a chromosomally abnormal embryo is incorrectly identified as a normal embryo, and transferred into the uterus. This technique can only assess the sampled tissue. The remainder and undiagnosed ...
Molecular Genetics and Genomics
... the other two had lost both phenotypes. Chromosomal exoB Tn5 insertions could not be obtained by following the plasmid incompatibility method (Ditta 1986). A disruption mutant of the chromosomal exoB gene was obtained by using gene sacB (Quandt and Hynes 1993), which renders cells unable to grow in ...
... the other two had lost both phenotypes. Chromosomal exoB Tn5 insertions could not be obtained by following the plasmid incompatibility method (Ditta 1986). A disruption mutant of the chromosomal exoB gene was obtained by using gene sacB (Quandt and Hynes 1993), which renders cells unable to grow in ...
Pourcel et al., Microbiology 2005
... Y. pseudotuberculosis represents an older, more diverged species from which Y. pestis probably emerged as a clone (Achtman et al., 1999). The three CRISPRs are present at least in some members of this species, and can be amplified using the Y. pestis primer pairs. We analysed the CRISPRs of nine str ...
... Y. pseudotuberculosis represents an older, more diverged species from which Y. pestis probably emerged as a clone (Achtman et al., 1999). The three CRISPRs are present at least in some members of this species, and can be amplified using the Y. pestis primer pairs. We analysed the CRISPRs of nine str ...
Article
... hybrids, which contain a complete haploid genome from each species, the presence of compatible alleles can mask the effects of (recessive) incompatible speciation genes. But in the haploid gametes produced by F1 hybrids, recessive speciation genes may be exposed, killing the gametes and thus prevent ...
... hybrids, which contain a complete haploid genome from each species, the presence of compatible alleles can mask the effects of (recessive) incompatible speciation genes. But in the haploid gametes produced by F1 hybrids, recessive speciation genes may be exposed, killing the gametes and thus prevent ...
Regulation and Flexibility of Genomic Imprinting
... and maize (Zea mays) have shown a strong correlation between silent or active states with epigenetic marks, such as DNA methylation and histone modifications, but the nature of the primary imprint has not been clearly established for all imprinted genes. Phenotypes and expression patterns of imprint ...
... and maize (Zea mays) have shown a strong correlation between silent or active states with epigenetic marks, such as DNA methylation and histone modifications, but the nature of the primary imprint has not been clearly established for all imprinted genes. Phenotypes and expression patterns of imprint ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.