Figure 20-6
... carrying them • They are generally called X and Y chromosomes, and differ in appearance, and genetic makeup (XX female; XY male) • During sexual reproduction the X and Y chromosomes behave as homologues © 2012 Pearson Education, Inc. ...
... carrying them • They are generally called X and Y chromosomes, and differ in appearance, and genetic makeup (XX female; XY male) • During sexual reproduction the X and Y chromosomes behave as homologues © 2012 Pearson Education, Inc. ...
... obtained from the cross Elgin × E420. The polymorphic fragment generated with primer UBC330 showed a 3:1 segregation ratio but was not linked to the Rps1 locus. Linkage analysis of the five RAPD markers that produce E420-specific bands along with two RFLP markers (Diers et al. 1992) relative to the ...
Molecular basis of the inflammatory response to adenovirus
... Adenoviridae are nonenveloped viruses with a 30– 40 kb linear double-stranded DNA genome. There are approximately 50 serotypes of adenoviridae with the group C viruses (serotypes 2 and 5) most extensively studied and developed for gene therapy applications. In addition, the application of replicatio ...
... Adenoviridae are nonenveloped viruses with a 30– 40 kb linear double-stranded DNA genome. There are approximately 50 serotypes of adenoviridae with the group C viruses (serotypes 2 and 5) most extensively studied and developed for gene therapy applications. In addition, the application of replicatio ...
SNP
... SNP: bi-allelic markers with 2 common nucleotide substitution alleles (0.1% of total SNPs is tri-allelic markers in TSC data). SNPs has lower mutation rate than do repeat sequences, but not as informative as microsatellite markers. detection methods for SNPs are potentially more suitable for genetic ...
... SNP: bi-allelic markers with 2 common nucleotide substitution alleles (0.1% of total SNPs is tri-allelic markers in TSC data). SNPs has lower mutation rate than do repeat sequences, but not as informative as microsatellite markers. detection methods for SNPs are potentially more suitable for genetic ...
Patterns of prokaryotic lateral gene transfers affecting parasitic
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
Patterns of prokaryotic lateral gene transfers affecting parasitic
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... iii) (4 pts) After constructing the expression vector, you transform the DNA into bacteria such that each bacteria receives one DNA molecule. To your surprise, you find that none of the bacterial cells produce peptide from the expression vector. Plasmid DNA is isolated from a number of different bac ...
Combining Whole-exome and RNA-Seq Data Improves the Quality
... are averaged for 92 single model analyses. For each category, the first percentage (bold font) specifies the fraction of all exonic mutations while the second percentage specifies the proportion with respect to the category above. ...
... are averaged for 92 single model analyses. For each category, the first percentage (bold font) specifies the fraction of all exonic mutations while the second percentage specifies the proportion with respect to the category above. ...
Characterization of the neurohypophysial hormone gene loci in
... elasmobranchs. Instead, its rectal gland comprises about 10 tubular structures located in the wall of post-valvular intestine [13]. However, elephant shark maintains its plasma levels of Na (~300 mmol l-1) and urea (~450 mmol l-1) similar to those in marine elasmobranchs [13]. ...
... elasmobranchs. Instead, its rectal gland comprises about 10 tubular structures located in the wall of post-valvular intestine [13]. However, elephant shark maintains its plasma levels of Na (~300 mmol l-1) and urea (~450 mmol l-1) similar to those in marine elasmobranchs [13]. ...
Uria_et_al_2006 _ADH paper
... ADHs in microbial genomes, and then cloning them in bacterial production strains, aiming at producing recombinant ADHs. In the absence of a genome sequence, finding a specific ADH gene is a difficult and challenging task. In this condition, total DNA is normally prepared from a microbe, and then dig ...
... ADHs in microbial genomes, and then cloning them in bacterial production strains, aiming at producing recombinant ADHs. In the absence of a genome sequence, finding a specific ADH gene is a difficult and challenging task. In this condition, total DNA is normally prepared from a microbe, and then dig ...
1 Defining the epigenetic mechanism of asymmetric cell division of
... mating-type locus (mat1) contains either a P or an M allele, and that determines the cell type, and two “donor” loci. The donor loci region, which is located 15.0-kb away from mat1, contains mat2-P and mat3-M cassettes. The donor cassettes are separated by an 11.0-kb region and 4.3-kb of that consis ...
... mating-type locus (mat1) contains either a P or an M allele, and that determines the cell type, and two “donor” loci. The donor loci region, which is located 15.0-kb away from mat1, contains mat2-P and mat3-M cassettes. The donor cassettes are separated by an 11.0-kb region and 4.3-kb of that consis ...
Large-scale Analysis of Pseudogenes in the Human Genome
... conservation, pseudogenes, especially those are transcribed, have been hypothesized to have regulatory roles [46]. Korneev and colleagues has reported that, in the neurons of mollusk Lymnaea stagnalis, a transcribed pseudogene of neural nitric oxide synthase (nNOS) suppresses the synthesis of nNOS p ...
... conservation, pseudogenes, especially those are transcribed, have been hypothesized to have regulatory roles [46]. Korneev and colleagues has reported that, in the neurons of mollusk Lymnaea stagnalis, a transcribed pseudogene of neural nitric oxide synthase (nNOS) suppresses the synthesis of nNOS p ...
Identity-by-descent filtering of exome sequence data for disease
... Fig. 1. HMM to identify regions identical by descent in exome sequencing data. (A) In siblings affected with an autosomal recessive disorder, both the maternal and the paternal haplotypes surrounding the disease gene are identical by descent (IBD = 2). It is not possible to measure the IBD = 2 state ...
... Fig. 1. HMM to identify regions identical by descent in exome sequencing data. (A) In siblings affected with an autosomal recessive disorder, both the maternal and the paternal haplotypes surrounding the disease gene are identical by descent (IBD = 2). It is not possible to measure the IBD = 2 state ...
Divergent Evolution of Function in the ROK Sugar
... electroporation. Transformed cells were plated on LuriaBertani plates containing ampicillin (150 µg/mL), chloramphenicol (25 µg/ mL), and kanamycin (40 µg/mL), and single colonies were isolated following overnight incubation at 37 °C. NcoI and EagI double digests of miniprep DNA prepared from indivi ...
... electroporation. Transformed cells were plated on LuriaBertani plates containing ampicillin (150 µg/mL), chloramphenicol (25 µg/ mL), and kanamycin (40 µg/mL), and single colonies were isolated following overnight incubation at 37 °C. NcoI and EagI double digests of miniprep DNA prepared from indivi ...
Genetically Essential and Nonessential a-Tubulin Genes Specify Functionally Interchangeable Proteins.
... were mapped not only so that we could determine whether they were linked but also because knowledge of the map positions of both genes was essential to the genetic analysis described in later sections. Both of these genes were assigned to chromosome 13 by blot hybridization of gels of intact yeast c ...
... were mapped not only so that we could determine whether they were linked but also because knowledge of the map positions of both genes was essential to the genetic analysis described in later sections. Both of these genes were assigned to chromosome 13 by blot hybridization of gels of intact yeast c ...
A Single Gene Causes Both Male Sterility and
... the GA19777USA transgene produced progeny significantly more often than males that inherited only the endogenous GA19777BOG, although fertility rescue was weak (Table 1). On the basis of these results, we conclude that GA19777 plays a role in hybrid male sterility. Surprisingly, GA19777USA transgeni ...
... the GA19777USA transgene produced progeny significantly more often than males that inherited only the endogenous GA19777BOG, although fertility rescue was weak (Table 1). On the basis of these results, we conclude that GA19777 plays a role in hybrid male sterility. Surprisingly, GA19777USA transgeni ...
Origin of the eukaryotic cell
... Another interesting phenomenon as a result of endosymbionts was the gene migration from the organelle genome to the nuclear genome, and thus the reduction of the organelle genome, since stable environment within eukaryotic cells has eliminated the unnecessary genes that used to be essential for free ...
... Another interesting phenomenon as a result of endosymbionts was the gene migration from the organelle genome to the nuclear genome, and thus the reduction of the organelle genome, since stable environment within eukaryotic cells has eliminated the unnecessary genes that used to be essential for free ...
Molecular Evolution in Nonrecombining Regions of the Drosophila
... level of nonsynonymous polymorphism relative to synonymous polymorphism, and a reduced incidence of positive selection. However, in Drosophila melanogaster and its relatives, these studies have mostly focused on the small dot (fourth) chromosome, where recombination is minimal or completely absent ( ...
... level of nonsynonymous polymorphism relative to synonymous polymorphism, and a reduced incidence of positive selection. However, in Drosophila melanogaster and its relatives, these studies have mostly focused on the small dot (fourth) chromosome, where recombination is minimal or completely absent ( ...
A molecular method for assessing meiofauna diversity in marine
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
Coordination of replication and transcription along a Drosophila
... hybridized labeled poly(A)-containing mRNA from asynchronous cells to the genomic microarray, using total genomic DNA as a control. The resulting pattern of expression along the chromosome was in close agreement with the predictions of inter- and intragenic sequences from the Drosophila annotation p ...
... hybridized labeled poly(A)-containing mRNA from asynchronous cells to the genomic microarray, using total genomic DNA as a control. The resulting pattern of expression along the chromosome was in close agreement with the predictions of inter- and intragenic sequences from the Drosophila annotation p ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.