human genome research
... Completion of this project will represent a remarkable achievement, especially considering that the first DNA sequencing methods were not developed until the 1970s. Such detailed knowledge of the human genome will open up new areas of research in basic biology, biomedicine, biotechnology and health ...
... Completion of this project will represent a remarkable achievement, especially considering that the first DNA sequencing methods were not developed until the 1970s. Such detailed knowledge of the human genome will open up new areas of research in basic biology, biomedicine, biotechnology and health ...
Supplementary Figures (doc 9746K)
... gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ start sites (donor site). The 5th and 6th categories are junctions whose splice sites are b ...
... gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ start sites (donor site). The 5th and 6th categories are junctions whose splice sites are b ...
Microarrays
... other non-targeted microorganisms. These results were in 100% agreement with reference methods. This study revealed that the ITS offers a higher resolution potential than the 16S rRNA gene. As the 16S rRNA gene, the ITS is present in multiple copies in most bacterial species. However, far less ITS s ...
... other non-targeted microorganisms. These results were in 100% agreement with reference methods. This study revealed that the ITS offers a higher resolution potential than the 16S rRNA gene. As the 16S rRNA gene, the ITS is present in multiple copies in most bacterial species. However, far less ITS s ...
Keverne et al (2001)
... at prepartum and postpartum. Milk ejection is controlled by oxytocin released from the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei in response to the suckling stimulus. Postpartum mutant mothers were found to have reduced oxytocin-positive neurons compared to the wild-type females ...
... at prepartum and postpartum. Milk ejection is controlled by oxytocin released from the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei in response to the suckling stimulus. Postpartum mutant mothers were found to have reduced oxytocin-positive neurons compared to the wild-type females ...
Cloning of the Papaya Chromoplast-Specific
... tomato CYC-b cDNA probe. Five BACs were identified by the SCAR marker and two by the tomato CYC-b cDNA probe. These seven BACs were mapped to a single fingerprint contig FPC-1648 of the papaya physical map (Supplemental Fig. S1; Yu et al., 2009). Colocalization of the flesh color-linked SCAR marker ...
... tomato CYC-b cDNA probe. Five BACs were identified by the SCAR marker and two by the tomato CYC-b cDNA probe. These seven BACs were mapped to a single fingerprint contig FPC-1648 of the papaya physical map (Supplemental Fig. S1; Yu et al., 2009). Colocalization of the flesh color-linked SCAR marker ...
PDF - FEMS Microbiology Letters
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
Repair of Site-Specific DNA Double-Strand Breaks in
... 1999), as postulated for yeast (Saccharomyces cerevisiae), has not yet been proven for higher eukaryotes (Schubert et al., 2011). Genetic transformation represents an outcome of erroneous DSB repair. Gene targeting (GT) on the basis of sequence homology between the transgene and the target locus is ...
... 1999), as postulated for yeast (Saccharomyces cerevisiae), has not yet been proven for higher eukaryotes (Schubert et al., 2011). Genetic transformation represents an outcome of erroneous DSB repair. Gene targeting (GT) on the basis of sequence homology between the transgene and the target locus is ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... Development of a genetic or transcriptional expression system in Chlamydia has been unsuccessful, due to unique difficulties inherent in working with Chlumydia. Unsuccessful attempts have been made to transcribe chlamydial genes in Escherichia coli, to culture Chlamydia outside host cells, and to am ...
... Development of a genetic or transcriptional expression system in Chlamydia has been unsuccessful, due to unique difficulties inherent in working with Chlumydia. Unsuccessful attempts have been made to transcribe chlamydial genes in Escherichia coli, to culture Chlamydia outside host cells, and to am ...
Locus in Salmonid Fishes Comparative Genome Analysis of the
... (O. mykiss), coho salmon (O. kisutch), and chinook salmon (O. tshawytscha; May et al. 1989; Du et al. 1993; Forbes et al. 1994; Prodöhl et al. 1994; Young et al. 1998; Nakayama et al. 1999; Sakamoto et al. 2000; Devlin et al. 2001; Zhang et al. 2001; Stein et al. 2002). Heteromorphic sex chromosomes ...
... (O. mykiss), coho salmon (O. kisutch), and chinook salmon (O. tshawytscha; May et al. 1989; Du et al. 1993; Forbes et al. 1994; Prodöhl et al. 1994; Young et al. 1998; Nakayama et al. 1999; Sakamoto et al. 2000; Devlin et al. 2001; Zhang et al. 2001; Stein et al. 2002). Heteromorphic sex chromosomes ...
Document
... • Meiosis reduces the number of chromosomes by half. • Daughter cells differ from parent, and each other. • Meiosis involves two divisions, Mitosis only one. ...
... • Meiosis reduces the number of chromosomes by half. • Daughter cells differ from parent, and each other. • Meiosis involves two divisions, Mitosis only one. ...
MER3 is required for normal meiotic crossover formation, but not for
... more than nine bivalents were observed in mer3 mutants (Fig. 5A). Thus, the frequency of chiasma is reduced dramatically in mer3, leading to the subsequent reduction of bivalent frequency. The number of bivalents was significantly reduced in mer3 mutants because of the decrease of chiasma number. Th ...
... more than nine bivalents were observed in mer3 mutants (Fig. 5A). Thus, the frequency of chiasma is reduced dramatically in mer3, leading to the subsequent reduction of bivalent frequency. The number of bivalents was significantly reduced in mer3 mutants because of the decrease of chiasma number. Th ...
Rolling circle transcription on smallest size double stranded DNA
... DNA nanotechnology DNA nanotechnology is the application of DNA as a material to construct nano scale patterns or structures as opposed the information carrying role the molecule fills in nature. DNA has diverse potential applications such as molecular computing, as a scaffold serving as structural ...
... DNA nanotechnology DNA nanotechnology is the application of DNA as a material to construct nano scale patterns or structures as opposed the information carrying role the molecule fills in nature. DNA has diverse potential applications such as molecular computing, as a scaffold serving as structural ...
cbb752-mg-spr10-bioinfo-intro
... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
Genetic characterization of the mitochondrial DNA - (BORA)
... on opposite DNA strands, as well as genes on the same strand (Wolstenholme, 1992b). Within the metazoans the mitochondrial genomes range in size from 14 – 42 kb (Crease, 1999). This variation in size can to some extent be due to differences in gene length, but in most cases it is a result of size di ...
... on opposite DNA strands, as well as genes on the same strand (Wolstenholme, 1992b). Within the metazoans the mitochondrial genomes range in size from 14 – 42 kb (Crease, 1999). This variation in size can to some extent be due to differences in gene length, but in most cases it is a result of size di ...
Developmental Validation of the Quantifiler Real-Time
... formula was determined using Microsoft Excel 2000. Each sample was then quantified by entering its background-subtracted numerical fluorescence value into the regression formula and solving for DNA concentration. Slot blot hybridization DNA quantification assays were perR formed using the QuantiBlo ...
... formula was determined using Microsoft Excel 2000. Each sample was then quantified by entering its background-subtracted numerical fluorescence value into the regression formula and solving for DNA concentration. Slot blot hybridization DNA quantification assays were perR formed using the QuantiBlo ...
The Euglena gracilis chloroplast rpoB gene
... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
Guanine-Plus-Cytosine Content of Rothia dentocaviosa
... To resolve this discrepancy, we determined the G+C contents of two American Type Culture Collection strains of R. dentocariosa and one clinical isolate. Strains ATCC 17931T (T = type strain) and ATCC 19426 were obtained from the American Type Culture Collection and were grown on casein soy agar supp ...
... To resolve this discrepancy, we determined the G+C contents of two American Type Culture Collection strains of R. dentocariosa and one clinical isolate. Strains ATCC 17931T (T = type strain) and ATCC 19426 were obtained from the American Type Culture Collection and were grown on casein soy agar supp ...
Human Ig heavy chain CDR3 regions in adult
... mature B cells generally needed four cycles more. PCR were saturated six cycles later and the cycles between these two timepoints were taken as the linear phase of amplification. This correlated well with a similar PCR on the constant region of Ig H chain genes, performed with a 59 Cµ primer (59AAGT ...
... mature B cells generally needed four cycles more. PCR were saturated six cycles later and the cycles between these two timepoints were taken as the linear phase of amplification. This correlated well with a similar PCR on the constant region of Ig H chain genes, performed with a 59 Cµ primer (59AAGT ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.