Mapping the histone code at hMLH1. - JScholarship
... of Protein A and Protein G agarose beads (Upstate USA, Inc.; 3 parts Protein A and 1 part Protein G with 200 µg/ml salmon sperm DNA and 0.5 mg/ml bovine serum albumin in TE buffer as a 50% gel slurry) for 1 hr at 4°C with agitation. The soluble chromatin fraction was collected, and 5 µl of either an ...
... of Protein A and Protein G agarose beads (Upstate USA, Inc.; 3 parts Protein A and 1 part Protein G with 200 µg/ml salmon sperm DNA and 0.5 mg/ml bovine serum albumin in TE buffer as a 50% gel slurry) for 1 hr at 4°C with agitation. The soluble chromatin fraction was collected, and 5 µl of either an ...
ACMG Standards and Guidelines for constitutional cytogenomic
... the parameters specific to their platform (number of consecutive probes, log2 ratios, SNP allele ratios, QC metrics, etc.) that are necessary to conclude that a copy number call represents a true CNV. As the functional resolution is a combination of probe density and number of probes necessary to id ...
... the parameters specific to their platform (number of consecutive probes, log2 ratios, SNP allele ratios, QC metrics, etc.) that are necessary to conclude that a copy number call represents a true CNV. As the functional resolution is a combination of probe density and number of probes necessary to id ...
Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates
... and a SapI site at the 3⬘ end of each gene. The PCR products, digested with NdeI and SapI, were cloned into the pTYB1 vector (New England BioLabs) digested with the same enzymes, to form the plasmids pHPgltX1 and -X2. The identity of the clones was determined by DNA sequencing. Analysis (http:兾兾tigr ...
... and a SapI site at the 3⬘ end of each gene. The PCR products, digested with NdeI and SapI, were cloned into the pTYB1 vector (New England BioLabs) digested with the same enzymes, to form the plasmids pHPgltX1 and -X2. The identity of the clones was determined by DNA sequencing. Analysis (http:兾兾tigr ...
Bacteriophage contamination: is there a simple method
... lysogenic for one phage are resistant to infection by the same phage type. However, as mentioned above, lysogenic strains may grow less efficiently than non-lysogenic bacteria, and synthesis of bioproducts in such strains may be decreased (WU 1998, JONES et al. 2000). Moreover, the potential problem ...
... lysogenic for one phage are resistant to infection by the same phage type. However, as mentioned above, lysogenic strains may grow less efficiently than non-lysogenic bacteria, and synthesis of bioproducts in such strains may be decreased (WU 1998, JONES et al. 2000). Moreover, the potential problem ...
CAIcal: A combined set of tools to assess codon usage adaptation
... length, total G+C content and G+C content at the three codon positions, and the effective number of codons [17]. (ii) The CAI of a DNA sequence or group of sequences. This index measures the adaptation of the synonymous codon usage of a gene to the synonymous codon usage of up to two reference sets ...
... length, total G+C content and G+C content at the three codon positions, and the effective number of codons [17]. (ii) The CAI of a DNA sequence or group of sequences. This index measures the adaptation of the synonymous codon usage of a gene to the synonymous codon usage of up to two reference sets ...
Cloning and functional characterization of temperature responsive
... Another reason of this potential in drought tolerance could be due to its high stomatal control enabling plants to minimize the transpiration rate by keeping also a high level of net CO2 assimilation under water stress conditions (Severino et al., 2012). These physiologic ...
... Another reason of this potential in drought tolerance could be due to its high stomatal control enabling plants to minimize the transpiration rate by keeping also a high level of net CO2 assimilation under water stress conditions (Severino et al., 2012). These physiologic ...
Bacteriophage contamination - Journal of Applied Genetics
... lysogenic for one phage are resistant to infection by the same phage type. However, as mentioned above, lysogenic strains may grow less efficiently than non-lysogenic bacteria, and synthesis of bioproducts in such strains may be decreased (WU 1998, JONES et al. 2000). Moreover, the potential problem ...
... lysogenic for one phage are resistant to infection by the same phage type. However, as mentioned above, lysogenic strains may grow less efficiently than non-lysogenic bacteria, and synthesis of bioproducts in such strains may be decreased (WU 1998, JONES et al. 2000). Moreover, the potential problem ...
The evolutionary history of human chromosome 7
... regions at 7q22 and 7p22 in African apes, but not in the homologous chromosome regions in orangutan and gibbon. Since a detailed analysis of the WBS orthologous region on mouse chromosome 5 provided no evidence of duplicated segments, the authors concluded that these segmental duplications are of re ...
... regions at 7q22 and 7p22 in African apes, but not in the homologous chromosome regions in orangutan and gibbon. Since a detailed analysis of the WBS orthologous region on mouse chromosome 5 provided no evidence of duplicated segments, the authors concluded that these segmental duplications are of re ...
FEMS Microbiology Letters
... respectively. Now an 1.1 kb nifJ probe, containing part of the earlier characterised 267 bp segment, was generated by inverse PCR from Anabaena 7119 and used in hybridisation experiments of EcoRV/HindIII-digested genomic DNA from A. variabilis. Two hybridisation signals were obtained at 3.5 kb and 3 ...
... respectively. Now an 1.1 kb nifJ probe, containing part of the earlier characterised 267 bp segment, was generated by inverse PCR from Anabaena 7119 and used in hybridisation experiments of EcoRV/HindIII-digested genomic DNA from A. variabilis. Two hybridisation signals were obtained at 3.5 kb and 3 ...
Novel Research Starts with GAPDH - Bio-Rad
... – inefficient – non-specific • Benefits of initial PCR: – cast a wide net – increase the pool of specific products ...
... – inefficient – non-specific • Benefits of initial PCR: – cast a wide net – increase the pool of specific products ...
Obligate phototrophy in cyanobacteria: more than a lack of sugar
... acid sequence identity with, and a predictable structure similar to, that of a family of non-phosphorylating sugar carriers largely distributed among prokaryotes and eukaryotes. It thus appeared probable that the carrier of other facultative phototrophic cyanobacteria using glucose as substrate woul ...
... acid sequence identity with, and a predictable structure similar to, that of a family of non-phosphorylating sugar carriers largely distributed among prokaryotes and eukaryotes. It thus appeared probable that the carrier of other facultative phototrophic cyanobacteria using glucose as substrate woul ...
The past, present and future of plant breeding
... our food production today. Simply eliminating ...
... our food production today. Simply eliminating ...
Opposite deletions/duplications of the X chromosome: two
... with one or the other. At paternal meiosis the X and the Y chromosomes pair at the Xp-Yp pseudoautosomal region but are free for the rest of their length. It has been demonstrated that this configuration favours refolding of the chromosomes into themselves, leading in turn to intrachromosome synapse ...
... with one or the other. At paternal meiosis the X and the Y chromosomes pair at the Xp-Yp pseudoautosomal region but are free for the rest of their length. It has been demonstrated that this configuration favours refolding of the chromosomes into themselves, leading in turn to intrachromosome synapse ...
High-order chromatin architecture determines the
... more detailed analyses than the current array-based approaches, allowing for greater mechanistic insight into SCNA formation. In particular, high-throughput whole-genome sequencing data will allow for both a high-resolution analysis of interchromosomal rearrangements and yield insight into the inter ...
... more detailed analyses than the current array-based approaches, allowing for greater mechanistic insight into SCNA formation. In particular, high-throughput whole-genome sequencing data will allow for both a high-resolution analysis of interchromosomal rearrangements and yield insight into the inter ...
Discovery and characterization of chromatin states for Please share
... all the states in this group were H3K27me3 and H3K9me3. State 40, covering 13% of the genome, was essentially devoid of any detected modifications, States 41–42 (25% of the genome) had a higher frequency for H3K9me3 than H3K27me3, while States 43–45 (26% of the genome) had a higher frequency for H3K ...
... all the states in this group were H3K27me3 and H3K9me3. State 40, covering 13% of the genome, was essentially devoid of any detected modifications, States 41–42 (25% of the genome) had a higher frequency for H3K9me3 than H3K27me3, while States 43–45 (26% of the genome) had a higher frequency for H3K ...
FREE Sample Here - Test bank Store
... 45) For geneticists, why is it important that genetic variability exist in the population under study? Answer: Genetic variation in individuals of a population is important for studying the inheritance pattern of those characteristics. If all the members of a population were identical for the trait ...
... 45) For geneticists, why is it important that genetic variability exist in the population under study? Answer: Genetic variation in individuals of a population is important for studying the inheritance pattern of those characteristics. If all the members of a population were identical for the trait ...
Slides
... Mapping Structural Variation in Humans >1 kb segments - Structural Variations are Common 40% of the genome -Structural Variations are involved in phenotype variation and disease - Until recently most methods for detection were low resolution (>50 kb) ...
... Mapping Structural Variation in Humans >1 kb segments - Structural Variations are Common 40% of the genome -Structural Variations are involved in phenotype variation and disease - Until recently most methods for detection were low resolution (>50 kb) ...
Alu repeat analysis in the complete human genome: trends and
... units separated by a poly ‘A’ stretch. The monomers, homologous to 7SL RNA, are absolutely identical except for a 30 bp insertion in the right monomer (Jelinek et al., 1980; Ullu and Tschudi, 1984). The 3 end of the Alu element has a long stretch of adenine residues, and is flanked by 4–10 bp of di ...
... units separated by a poly ‘A’ stretch. The monomers, homologous to 7SL RNA, are absolutely identical except for a 30 bp insertion in the right monomer (Jelinek et al., 1980; Ullu and Tschudi, 1984). The 3 end of the Alu element has a long stretch of adenine residues, and is flanked by 4–10 bp of di ...
Fusobacterium pseudonecrophorurn Is a Synonym for Fusobacten
... while biovar C was “biochemically similar to the other two biovars,” it was distinct in genetic terms and was “worthy of species designation.” This species was first described in 1927 from puerperal infection of women and named Actinomyces pseudonecrophorus (5). Subsequently, it was renamed Sphaerop ...
... while biovar C was “biochemically similar to the other two biovars,” it was distinct in genetic terms and was “worthy of species designation.” This species was first described in 1927 from puerperal infection of women and named Actinomyces pseudonecrophorus (5). Subsequently, it was renamed Sphaerop ...
the genome of herpes simplex virus: structure, replication and
... near the centre of U l (Kaerner et al. 1979; Vlazny & Frenkel, 1981; Spaete & Frenkel, 1982). It was also shown that when monomer units from either class of defective were introduced into cells in the presence of wild-type virus, these could serve as ‘seeds’ for regeneration of tandemly repeated def ...
... near the centre of U l (Kaerner et al. 1979; Vlazny & Frenkel, 1981; Spaete & Frenkel, 1982). It was also shown that when monomer units from either class of defective were introduced into cells in the presence of wild-type virus, these could serve as ‘seeds’ for regeneration of tandemly repeated def ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.