Complete Elimination of Endosymbiotic Algae from Paramecium
... We have shown that an appropriate exposure of P. bursaria to paraquat produces symbiotic algae-free strains without cellular damage or physiological distortion of cell division and conjugation. The newly established algae-free paramecia showed the same growth rate as that of normal green paramecia. ...
... We have shown that an appropriate exposure of P. bursaria to paraquat produces symbiotic algae-free strains without cellular damage or physiological distortion of cell division and conjugation. The newly established algae-free paramecia showed the same growth rate as that of normal green paramecia. ...
Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... alleles, probably deriving from specific individual mutations that are characterized by nonpigmentation of the leaves (Bartley, 2005). A similar effect was observed in F2 progenies, resulting from self-fertilization of F1 plants from Pa 121 x SIC 802 and Pa 121 x Pa 169 (Yamada et al., 1982). One fo ...
... alleles, probably deriving from specific individual mutations that are characterized by nonpigmentation of the leaves (Bartley, 2005). A similar effect was observed in F2 progenies, resulting from self-fertilization of F1 plants from Pa 121 x SIC 802 and Pa 121 x Pa 169 (Yamada et al., 1982). One fo ...
Revista agronomica del Noroeste Argentino
... The nir test was used also to calculate the most probable number (MPN) of denitrifying Azospirillum from root samples of each locality of the two regions considered in this work, following the prescriptions of Döbereiner et al. (1995) and by using the McCrady table for 5 replicates. PCR amplificatio ...
... The nir test was used also to calculate the most probable number (MPN) of denitrifying Azospirillum from root samples of each locality of the two regions considered in this work, following the prescriptions of Döbereiner et al. (1995) and by using the McCrady table for 5 replicates. PCR amplificatio ...
Genomic Organization of Evolutionarily Correlated Genes in
... benchmark data set consisting of 2254 proteincoding genes contributing to 22,500 gene pairs. 7 These pairs had been identified by comparing 105 bacterial genomes on the basis of two types of evolutionary correlations: 7 a tendency to be located close together in many genomes, independently of their r ...
... benchmark data set consisting of 2254 proteincoding genes contributing to 22,500 gene pairs. 7 These pairs had been identified by comparing 105 bacterial genomes on the basis of two types of evolutionary correlations: 7 a tendency to be located close together in many genomes, independently of their r ...
A structural determinant in the uracil DNA glycosylase superfamily
... UNG enzymes, we identified Lys-68 as a potential structural element located outside of motifs 1 and 2 that can determine the UDG activity on A/U base pairs. Mutational analysis presented here demonstrates that a K68N substitution not only allows E. coli MUG to act on A/U base pairs, but also increas ...
... UNG enzymes, we identified Lys-68 as a potential structural element located outside of motifs 1 and 2 that can determine the UDG activity on A/U base pairs. Mutational analysis presented here demonstrates that a K68N substitution not only allows E. coli MUG to act on A/U base pairs, but also increas ...
Damage Control: The Pleiotropy of DNA Repair Genes
... cells. This result has been confirmed and extended to cells in the eye imaginal disc (M. Brodsky and G. M. Rubin, personal communication). Thus the function of the MEI-41 protein may not be in the repair of damage per se, but in triggering a DNA damage-dependent cellcycle checkpoint. Activation of t ...
... cells. This result has been confirmed and extended to cells in the eye imaginal disc (M. Brodsky and G. M. Rubin, personal communication). Thus the function of the MEI-41 protein may not be in the repair of damage per se, but in triggering a DNA damage-dependent cellcycle checkpoint. Activation of t ...
Chromosomal Theory and Genetic Linkage
... chance of being the parental type (inheriting the same combination of traits as the parents) or a nonparental type (inheriting a dierent combination of traits than the parents). In (b), two genes are very close together on the same chromosome so that no crossing over occurs between them. The genes ...
... chance of being the parental type (inheriting the same combination of traits as the parents) or a nonparental type (inheriting a dierent combination of traits than the parents). In (b), two genes are very close together on the same chromosome so that no crossing over occurs between them. The genes ...
Chromosomal Theory and Genetic Linkage
... chance of being the parental type (inheriting the same combination of traits as the parents) or a nonparental type (inheriting a dierent combination of traits than the parents). In (b), two genes are very close together on the same chromosome so that no crossing over occurs between them. The genes ...
... chance of being the parental type (inheriting the same combination of traits as the parents) or a nonparental type (inheriting a dierent combination of traits than the parents). In (b), two genes are very close together on the same chromosome so that no crossing over occurs between them. The genes ...
Practice exam 3 key
... (3 pts) Leaves single-stranded overhangs (or 'sticky ends') (1 pt); these ends are complementary (will hybridize) (1 pt); can be used to join 2 DNA fragments cut with EcoRI (with the same restriction enzyme) (1 pt). ...
... (3 pts) Leaves single-stranded overhangs (or 'sticky ends') (1 pt); these ends are complementary (will hybridize) (1 pt); can be used to join 2 DNA fragments cut with EcoRI (with the same restriction enzyme) (1 pt). ...
CtrA mediates a DNA replication checkpoint that prevents cell
... promoter PQA at the end of S phase when ftsZ transcription is repressed by CtrA (Sackett et al., 1998). This suggested the possibility that coupling of PQA transcription to DNA replication could provide a checkpoint to link the transcription of late cell division genes to DNA replication (Sackett et ...
... promoter PQA at the end of S phase when ftsZ transcription is repressed by CtrA (Sackett et al., 1998). This suggested the possibility that coupling of PQA transcription to DNA replication could provide a checkpoint to link the transcription of late cell division genes to DNA replication (Sackett et ...
A novel species of thermoacidophilic archaeon, Sulfolobus
... (Promega). The reverse transcription reaction contained (pl-l): 20 ng RNA template, 0-5 U AMV reverse transcriptase, 1 pmol primer, 5 nmol MgC1, and 1 nmol dNTPs. The reaction was incubated at 42 "C for 30 min. Three independent cDNA products were synthesized and used as templates for the PCR. PCR a ...
... (Promega). The reverse transcription reaction contained (pl-l): 20 ng RNA template, 0-5 U AMV reverse transcriptase, 1 pmol primer, 5 nmol MgC1, and 1 nmol dNTPs. The reaction was incubated at 42 "C for 30 min. Three independent cDNA products were synthesized and used as templates for the PCR. PCR a ...
15_Lecture_Stock
... • A linkage map is a genetic map of a chromosome based on recombination frequencies • Distances between genes can be expressed as map units; one map unit, or centimorgan, represents a 1% recombination frequency • Map units indicate relative distance and order, not precise locations of genes ...
... • A linkage map is a genetic map of a chromosome based on recombination frequencies • Distances between genes can be expressed as map units; one map unit, or centimorgan, represents a 1% recombination frequency • Map units indicate relative distance and order, not precise locations of genes ...
Chromosome Theory
... the F2 generation were males Without this vital data on the association of white eyes with being male, the gene for white eyes could have been seen as a simple recessive trait on an autosome This illustrates the importance of recording all the data possible and being alert to the possibility of ...
... the F2 generation were males Without this vital data on the association of white eyes with being male, the gene for white eyes could have been seen as a simple recessive trait on an autosome This illustrates the importance of recording all the data possible and being alert to the possibility of ...
Highly precise and developmentally programmed genome
... thousands of short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these genome rearrangements are initiated by the introduction of developmentally programmed DNA double-strand breaks (DSBs), which ...
... thousands of short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these genome rearrangements are initiated by the introduction of developmentally programmed DNA double-strand breaks (DSBs), which ...
The Drosophila Gene Disruption Project: Progress
... ABSTRACT The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for a ...
... ABSTRACT The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for a ...
Chapter 8: Variation in Chromosome Structure and Number
... 8.6 Variation in the Number of Chromosomes Within A Set: Aneuploidy Overview Changes in chromosome number also have an effect on gene expression and the phenotype of the organism. Once again, the most common problems with the next two sections occurs in the terminology. However, the terminology for ...
... 8.6 Variation in the Number of Chromosomes Within A Set: Aneuploidy Overview Changes in chromosome number also have an effect on gene expression and the phenotype of the organism. Once again, the most common problems with the next two sections occurs in the terminology. However, the terminology for ...
The Gene Gateway Workbook
... freely available on the Web. It should take about 3 hours to complete all five activities. The workbook activities were derived from more detailed guides and tutorials available at the Gene Gateway Web site (www.DOEgenomes.org/genegateway). The Gene Gateway Web site was created as a resource for lea ...
... freely available on the Web. It should take about 3 hours to complete all five activities. The workbook activities were derived from more detailed guides and tutorials available at the Gene Gateway Web site (www.DOEgenomes.org/genegateway). The Gene Gateway Web site was created as a resource for lea ...
Deciphering the role of DNA methylation in multiple sclerosis
... cell life. DNA methylation mainly occurs at regions where a guanine accompanies the cytosine, forming a dinucleotide. Hundreds of these dinucleotides are found repetitively in gene promoters, as CpG islands. Hypermethylation of these sites leads to silencing of the gene, by not allowing transcriptio ...
... cell life. DNA methylation mainly occurs at regions where a guanine accompanies the cytosine, forming a dinucleotide. Hundreds of these dinucleotides are found repetitively in gene promoters, as CpG islands. Hypermethylation of these sites leads to silencing of the gene, by not allowing transcriptio ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... bacterial genome without bacterial cell lysis. Bactericidal Phage therapy is an application of phages in the treatment of bacterial infections based on the concept the bacteriophages are bacterial specific and bactericidal. The arising bacterial resistance to traditionally used antibacterial and the ...
... bacterial genome without bacterial cell lysis. Bactericidal Phage therapy is an application of phages in the treatment of bacterial infections based on the concept the bacteriophages are bacterial specific and bactericidal. The arising bacterial resistance to traditionally used antibacterial and the ...
How dormant origins promote complete genome replication
... [41]. It shows how dormant origins protect against double fork stalls (Figure 3b) that leave unreplicatable sections of DNA between them. Interestingly, the model shows that the density of licensed origins on DNA determines the degree of protection against double fork stalling, with the efficiency o ...
... [41]. It shows how dormant origins protect against double fork stalls (Figure 3b) that leave unreplicatable sections of DNA between them. Interestingly, the model shows that the density of licensed origins on DNA determines the degree of protection against double fork stalling, with the efficiency o ...
Stochastic processes and Markov chains (part II)
... Application: motifs The binding sites of a transciption factor (that may regulate multiple genes) share certain sequence patterns, motifs. p factors and motifs are known. Hence,, a Not all transcription high occurrence of a particular sequence pattern in the upstream regions of a gene may indicate ...
... Application: motifs The binding sites of a transciption factor (that may regulate multiple genes) share certain sequence patterns, motifs. p factors and motifs are known. Hence,, a Not all transcription high occurrence of a particular sequence pattern in the upstream regions of a gene may indicate ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.