Functional genomics of plant photosynthesis in
... To study the oxidizing side of PSI, insertional mutagenesis was used to generate a mutant in the nuclear psaF gene26. Analysis of the mutant showed that PsaF is important for the docking of plastocyanin to PSI. The positively charged N-terminal domain of PsaF in Chlamydomonas and plants is not prese ...
... To study the oxidizing side of PSI, insertional mutagenesis was used to generate a mutant in the nuclear psaF gene26. Analysis of the mutant showed that PsaF is important for the docking of plastocyanin to PSI. The positively charged N-terminal domain of PsaF in Chlamydomonas and plants is not prese ...
Text S1: Genome-Wide High-Resolution Mapping of UV
... PG311 has MATa, but lacks MATα information, the strain is incapable of undergoing meiosis and can be synchronized in G1 using α-factor, as described below. We also examined the frequency of UVinduced mitotic crossovers in PSL101, the progenitor diploid of PG311. This strain is identical to PG311 exc ...
... PG311 has MATa, but lacks MATα information, the strain is incapable of undergoing meiosis and can be synchronized in G1 using α-factor, as described below. We also examined the frequency of UVinduced mitotic crossovers in PSL101, the progenitor diploid of PG311. This strain is identical to PG311 exc ...
pDsRed-Monomer Vector Information
... excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence has been human codon-optimized for high expression in mammalian cells ...
... excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence has been human codon-optimized for high expression in mammalian cells ...
Definition
... After domestication of food crops and wild animals, man moved on to other new observations like cheese, curd, etc. Certainly, cheese can be considered as one of the first direct products (or by-product) of biotechnology, because it was prepared by adding rennet (an enzyme found in the stomach of cal ...
... After domestication of food crops and wild animals, man moved on to other new observations like cheese, curd, etc. Certainly, cheese can be considered as one of the first direct products (or by-product) of biotechnology, because it was prepared by adding rennet (an enzyme found in the stomach of cal ...
HL7 V2.5.1 Genetic Test Result Message
... genomic and healthcare IT data standards may use this guide to extend these standards for support of clinical sequencing. Users of this guide must be familiar with the details of HL7 message construction and processing. This guide is not intended to be a tutorial on that subject. ...
... genomic and healthcare IT data standards may use this guide to extend these standards for support of clinical sequencing. Users of this guide must be familiar with the details of HL7 message construction and processing. This guide is not intended to be a tutorial on that subject. ...
Genes: Structure, Replication, and Mutation
... information on plasmids and the nature of genetic recombination in microorganisms. These three chapters provide the background needed for understanding the material in Part Five: recombinant DNA technology (chapter 14) and microbial genomics (chapter 15). Geneticists, including microbial geneticists ...
... information on plasmids and the nature of genetic recombination in microorganisms. These three chapters provide the background needed for understanding the material in Part Five: recombinant DNA technology (chapter 14) and microbial genomics (chapter 15). Geneticists, including microbial geneticists ...
Mcbio 316: Exam 2 ANSWER KEY (10) 1. Proteins encoded by the
... a. What does this suggest about the properties of these two plasmids? [Briefly explain your answer.] ANSWER: The two plasmids are probably incompatible, so only one of the plasmids can be stably maintained in the cell. Hence, selection for the second plasmid results in loss in the first plasmid. b. ...
... a. What does this suggest about the properties of these two plasmids? [Briefly explain your answer.] ANSWER: The two plasmids are probably incompatible, so only one of the plasmids can be stably maintained in the cell. Hence, selection for the second plasmid results in loss in the first plasmid. b. ...
Uracil in DNA – occurrence, consequences and repair
... The present review will focus on the repair of one of the most common lesions in DNA, uracil. However, since several of the known uracil-DNA glycosylases (UDGs) are not strictly uracil-specific, the repair of some uracil analogues (Figure 1), namely 5-hydroxymethyluracil (5-hmU), 3,N4-ethenocytosine ...
... The present review will focus on the repair of one of the most common lesions in DNA, uracil. However, since several of the known uracil-DNA glycosylases (UDGs) are not strictly uracil-specific, the repair of some uracil analogues (Figure 1), namely 5-hydroxymethyluracil (5-hmU), 3,N4-ethenocytosine ...
Organization and Synthesis of DNA
... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
Correlation of amino acid preference and
... Three types of analysis are conducted. At the genome level, crossvalidation is used to demonstrate the predictive capability of amino acid preference of viral genome type. Next at the sequence level, two types of resampling analysis are used to investigate the correlation of amino acid preference an ...
... Three types of analysis are conducted. At the genome level, crossvalidation is used to demonstrate the predictive capability of amino acid preference of viral genome type. Next at the sequence level, two types of resampling analysis are used to investigate the correlation of amino acid preference an ...
Experimental procedures (detailed)
... 68°C 6 min). Optimal LD PCR cycle number was determined empirically to ensure all cDNA samples were in exponential amplification phase. Usually, one cycle less than the plateau of the PCR reaction was used to amplify experimental double-stranded cDNA from the prepared SMART first-strand cDNA. ...
... 68°C 6 min). Optimal LD PCR cycle number was determined empirically to ensure all cDNA samples were in exponential amplification phase. Usually, one cycle less than the plateau of the PCR reaction was used to amplify experimental double-stranded cDNA from the prepared SMART first-strand cDNA. ...
Classification of plant-pathogenic mycoplasma
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
Determination of the entire sequence of turtle CR1: the first open
... Fragments of approximately 5OO-1,000 bp, obtained as products of PCR, were isolated from an agarose gel and fractionated on SizeSepTM 400 Spun Columns (Pharmacia, Uppsala, Sweden) to remove fragments shorter than 400 bp. Longer fragments were ligated into the pUC 18 or pUC 19 vector, and then the nu ...
... Fragments of approximately 5OO-1,000 bp, obtained as products of PCR, were isolated from an agarose gel and fractionated on SizeSepTM 400 Spun Columns (Pharmacia, Uppsala, Sweden) to remove fragments shorter than 400 bp. Longer fragments were ligated into the pUC 18 or pUC 19 vector, and then the nu ...
controversy and its implications Genetic hitchhiking versus
... distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An important question has been whether BGS alone can account for the patterns of variation we observe, or whether positive selection also needs to be invoked. Although numerous attempts have been made, the con ...
... distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An important question has been whether BGS alone can account for the patterns of variation we observe, or whether positive selection also needs to be invoked. Although numerous attempts have been made, the con ...
Smallest critical region for microcephaly in a patient with mosaic ring
... Microcephaly is relatively common among developmentally delayed children. Four single etiologic genes have been identified. Microcephaly is also associated with at least 7 loci (Kinsman and Johnston, 2011) and is commonly observed in ring chromosome 13, or r(13) (Brandt et al., 1992; Bedoyan et al., ...
... Microcephaly is relatively common among developmentally delayed children. Four single etiologic genes have been identified. Microcephaly is also associated with at least 7 loci (Kinsman and Johnston, 2011) and is commonly observed in ring chromosome 13, or r(13) (Brandt et al., 1992; Bedoyan et al., ...
The trimethoprim-resistant dihydrofolate reductase associated with
... had copurified with the native R388 dihydrofolate reductase on the first Sephadex G-7 5 column, the pooled enzyme fractions were denatured by boiling in 6 M guanidinium chloride and chromatagraphed in Sephadex G-150 in the presence of 6 M urea (Fig. IB) . The R388 dihydrofolate reductase disaggregat ...
... had copurified with the native R388 dihydrofolate reductase on the first Sephadex G-7 5 column, the pooled enzyme fractions were denatured by boiling in 6 M guanidinium chloride and chromatagraphed in Sephadex G-150 in the presence of 6 M urea (Fig. IB) . The R388 dihydrofolate reductase disaggregat ...
24.5 Nucleic Acids
... Applications in Medicine In the past, human insulin was not available for this purpose. Pig insulin, which is quite similar, was used as a substitute. • Today, diabetic patients use the human form of insulin produced by bacteria that have been altered by recombinant DNA technology. • Use of this ins ...
... Applications in Medicine In the past, human insulin was not available for this purpose. Pig insulin, which is quite similar, was used as a substitute. • Today, diabetic patients use the human form of insulin produced by bacteria that have been altered by recombinant DNA technology. • Use of this ins ...
Hypercholesterolemia
... To detect the FH mutation, DNA is obtained from the patient’s blood or saliva; the section of DNA containing the LDL receptor gene is then amplified by the polymerase chain reaction (PCR). The amplified DNA is analyzed to see if there is a mutation. To analyze the DNA, investigators use restriction en ...
... To detect the FH mutation, DNA is obtained from the patient’s blood or saliva; the section of DNA containing the LDL receptor gene is then amplified by the polymerase chain reaction (PCR). The amplified DNA is analyzed to see if there is a mutation. To analyze the DNA, investigators use restriction en ...
Microcin B17 Blocks DNA Replication and Induces
... 10 mM-MgSO,, 0.2% glucose and 17' Difco agar. Lambda phage was assayed on LB plates lacking yeast extract and containing 1.2:/, agar. 5-bromo-4-chloro-3-indolyl P-D-Galactoside (X-gal) was used at 40 pg ml-I . Genetic methods. Transformation with plasmid DNA was done as described by Dagert & Ehrlich ...
... 10 mM-MgSO,, 0.2% glucose and 17' Difco agar. Lambda phage was assayed on LB plates lacking yeast extract and containing 1.2:/, agar. 5-bromo-4-chloro-3-indolyl P-D-Galactoside (X-gal) was used at 40 pg ml-I . Genetic methods. Transformation with plasmid DNA was done as described by Dagert & Ehrlich ...
Real-time Quantification of HER2/neu Gene Amplification by
... nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast pre-screening with high throughput that is able to deal with formalin-fixed archieved material, PCR might be the best choice. The PCR approaches published so far hav ...
... nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast pre-screening with high throughput that is able to deal with formalin-fixed archieved material, PCR might be the best choice. The PCR approaches published so far hav ...
Lack of biological significance in the `linguistic features` of
... 2 of the original paper of Mantegna et al. (2). To be concrete, we present here (in Fig. 1a) the corresponding graphs, for 6-tuples, of the human sequence HSRETBLAS (1.5% coding) and the Escherichia coli sequence ECUW89 (82.1% coding) DNAs, as also studied by Mantegna et al. (2). (The mentioned acro ...
... 2 of the original paper of Mantegna et al. (2). To be concrete, we present here (in Fig. 1a) the corresponding graphs, for 6-tuples, of the human sequence HSRETBLAS (1.5% coding) and the Escherichia coli sequence ECUW89 (82.1% coding) DNAs, as also studied by Mantegna et al. (2). (The mentioned acro ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.