Candidatus Paenicardinium endonii
... of the cell, whereas some extended part way. The width of MLSs that we determined (17 nm) was similar to that of Endo (1979), but wider than the 14–15 nm reported by Shepherd et al. (1973). These differences might be due to artefacts of fixation and embedding or calibration of instruments. We observ ...
... of the cell, whereas some extended part way. The width of MLSs that we determined (17 nm) was similar to that of Endo (1979), but wider than the 14–15 nm reported by Shepherd et al. (1973). These differences might be due to artefacts of fixation and embedding or calibration of instruments. We observ ...
Scientific Advisory Board
... sequence of increasing values of the penalty parameter λ, thus no need to use CV or GCV type criteria to determine λ. • Initial value: all β’s are set to zero • Each iteration modifies just one of the m dimensions, criteria to choose which dimension to update involves the gradient of the association ...
... sequence of increasing values of the penalty parameter λ, thus no need to use CV or GCV type criteria to determine λ. • Initial value: all β’s are set to zero • Each iteration modifies just one of the m dimensions, criteria to choose which dimension to update involves the gradient of the association ...
PDF - Blood Journal
... difficult task in most higher vertebrates. Current research has indicated the pufferfish (Fugu rubripes and the closely related Spheroides nephelus) as an ideal species for just this task because it has a relatively compact genome of 400 Mb, approximately 7.5 times smaller than the human genome.1,2 ...
... difficult task in most higher vertebrates. Current research has indicated the pufferfish (Fugu rubripes and the closely related Spheroides nephelus) as an ideal species for just this task because it has a relatively compact genome of 400 Mb, approximately 7.5 times smaller than the human genome.1,2 ...
Cloning and expression of the phosphotriesterase
... pGEM-T Easy (Promega) to generate the plasmid pGhocA5. A 3h region of hocA was also amplified by PCR with pBSRK7(1) as a template, using the primers RK7T3f (5h-GAACTTGAACAATCAGCCTTA-3h) and the T7 vector primer (5h-TAATACGACTCACTATAGGGAGA-3h). This generated an 800 bp fragment that was cloned into p ...
... pGEM-T Easy (Promega) to generate the plasmid pGhocA5. A 3h region of hocA was also amplified by PCR with pBSRK7(1) as a template, using the primers RK7T3f (5h-GAACTTGAACAATCAGCCTTA-3h) and the T7 vector primer (5h-TAATACGACTCACTATAGGGAGA-3h). This generated an 800 bp fragment that was cloned into p ...
Massive Changes in Genome Architecture Accompany
... of the degree to which changes in chromosome structure are involved in the suppression of recombination, made this organism an ideal candidate for a genome sequencing project. This sequencing project was undertaken by the Joint Genome Institute and involved the sequencing and assembly of two genomes ...
... of the degree to which changes in chromosome structure are involved in the suppression of recombination, made this organism an ideal candidate for a genome sequencing project. This sequencing project was undertaken by the Joint Genome Institute and involved the sequencing and assembly of two genomes ...
Metalloenzyme Functions
... supplement to the COGs is available, in which proteins encoded in the genomes of two multicellular eukaryotes, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, and shared with bacteria and/or archaea were included. The new features added to the COG database include info ...
... supplement to the COGs is available, in which proteins encoded in the genomes of two multicellular eukaryotes, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, and shared with bacteria and/or archaea were included. The new features added to the COG database include info ...
2- pcr primer design and reaction optimisation
... The specific complementary association due to hydrogen bonding of singlestranded nucleic acids is referred to as "annealing": two complementary sequences will form hydrogen bonds between their complementary bases (G to C, and A to T or U) and form a stable double-stranded, anti-parallel "hybrid" mol ...
... The specific complementary association due to hydrogen bonding of singlestranded nucleic acids is referred to as "annealing": two complementary sequences will form hydrogen bonds between their complementary bases (G to C, and A to T or U) and form a stable double-stranded, anti-parallel "hybrid" mol ...
Tissue-specific spatial organization of genomes
... For quantitative analysis of positioning, we first measured the distance between the nuclear center and the center of mass of each chromosome signal as an indicator of its radial position in two-dimensional (2D) projections of three-dimensional (3D) image stacks as previously described (Figure 1b; s ...
... For quantitative analysis of positioning, we first measured the distance between the nuclear center and the center of mass of each chromosome signal as an indicator of its radial position in two-dimensional (2D) projections of three-dimensional (3D) image stacks as previously described (Figure 1b; s ...
Kein Folientitel
... antibodies is routine matter in the art is not followed by the opposition division. An antibody that specifically recognises V28 is understood to mean an antibody that does not recognise any other protein. The generation of such antibodies is not considered a routine matter given the labour intensiv ...
... antibodies is routine matter in the art is not followed by the opposition division. An antibody that specifically recognises V28 is understood to mean an antibody that does not recognise any other protein. The generation of such antibodies is not considered a routine matter given the labour intensiv ...
MyTaxa: an advanced taxonomic classifier for genomic and
... the 16S rRNA gene, which serves as the best marker for taxonomic identification due to the availability of a large database of 16S rRNA gene sequences from uncultured organisms (8,9), is often missed or not assembled during single-cell (and WGS metagenomic) approaches (10). The 16S rRNA gene also pro ...
... the 16S rRNA gene, which serves as the best marker for taxonomic identification due to the availability of a large database of 16S rRNA gene sequences from uncultured organisms (8,9), is often missed or not assembled during single-cell (and WGS metagenomic) approaches (10). The 16S rRNA gene also pro ...
Transposable elements in Escherichia coli antimicrobial resistance
... the transposition process, they are automatically amplified, with a large copy number, restricted only by the carrying capacity of their environment (i.e. the genome) [1]. Transposons often persist in genomes over millions of years. This requires an exquisite balance between replication and suppress ...
... the transposition process, they are automatically amplified, with a large copy number, restricted only by the carrying capacity of their environment (i.e. the genome) [1]. Transposons often persist in genomes over millions of years. This requires an exquisite balance between replication and suppress ...
- Philsci
... Paralogues cannot be used to trace the lineage of two different organisms in terms of a shared ancestry, but are instead used to identify the historical events which led to the homology within an organism, in terms of the gene duplication events. Homology of one type does not necessitate similarity ...
... Paralogues cannot be used to trace the lineage of two different organisms in terms of a shared ancestry, but are instead used to identify the historical events which led to the homology within an organism, in terms of the gene duplication events. Homology of one type does not necessitate similarity ...
Integrating genome and transcriptome resources into the TreeGenes
... http://pinegenome.org/pinerefseq/ ...
... http://pinegenome.org/pinerefseq/ ...
Structure, expression and chromosomal location of the Oct
... between t- (4.73 kb) and wild-type (6.2 kb) and Southern blots of several t-haplotypes congenic on C3H (data not shown). The entire cDNA of the Juhachi clone of Oct-4 (Sch61er et al., 1990b) is contained on the 4.73 kb t-derived B a m H I fragment (Fig. 4A). Based on the published cDNA sequence of t ...
... between t- (4.73 kb) and wild-type (6.2 kb) and Southern blots of several t-haplotypes congenic on C3H (data not shown). The entire cDNA of the Juhachi clone of Oct-4 (Sch61er et al., 1990b) is contained on the 4.73 kb t-derived B a m H I fragment (Fig. 4A). Based on the published cDNA sequence of t ...
Role for CCG-trinucleotide repeats in the pathogenesis of chronic
... hybridization buffer40 overnight at 50°C. Membranes were washed in 2⫻SSC–0.1% sodium dodecyl sulfate at room temperature and in 0.2⫻SSC– 0.1% sodium dodecyl sulfate at 55°C for 15 minutes each. A PAC contig was constructed between D11S1897 and D11S2105 by polymerase chain reaction (PCR) of the RPCI1 ...
... hybridization buffer40 overnight at 50°C. Membranes were washed in 2⫻SSC–0.1% sodium dodecyl sulfate at room temperature and in 0.2⫻SSC– 0.1% sodium dodecyl sulfate at 55°C for 15 minutes each. A PAC contig was constructed between D11S1897 and D11S2105 by polymerase chain reaction (PCR) of the RPCI1 ...
Functional genomics of plant photosynthesis in
... To study the oxidizing side of PSI, insertional mutagenesis was used to generate a mutant in the nuclear psaF gene26. Analysis of the mutant showed that PsaF is important for the docking of plastocyanin to PSI. The positively charged N-terminal domain of PsaF in Chlamydomonas and plants is not prese ...
... To study the oxidizing side of PSI, insertional mutagenesis was used to generate a mutant in the nuclear psaF gene26. Analysis of the mutant showed that PsaF is important for the docking of plastocyanin to PSI. The positively charged N-terminal domain of PsaF in Chlamydomonas and plants is not prese ...
The Organellar Genome and Metabolic Potential
... The organellar DNA was isolated from the total DNA by pulsed field gel electrophoresis. This organellar DNA was used for a partial Sau 3A I digest and cloning in the Bam H1 restriction site from the vector pUC18c. From this library, 500 clones were sequenced in both directions and additional 500 in ...
... The organellar DNA was isolated from the total DNA by pulsed field gel electrophoresis. This organellar DNA was used for a partial Sau 3A I digest and cloning in the Bam H1 restriction site from the vector pUC18c. From this library, 500 clones were sequenced in both directions and additional 500 in ...
B. thuringiensis kurstaki
... separate the plasmids to different sizes. Figure 12.3 Bacillus thuringiensis kurstaki contains an insecticidal protoxin gene on one of 7 different plasmids (2, 7.4, 8.2, 14.4, 45 and 71 kb in length. ...
... separate the plasmids to different sizes. Figure 12.3 Bacillus thuringiensis kurstaki contains an insecticidal protoxin gene on one of 7 different plasmids (2, 7.4, 8.2, 14.4, 45 and 71 kb in length. ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.