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... For simplicity, whole reaction is split into two parts: ...
Genetic Control of Meat Quality Traits
Genetic Control of Meat Quality Traits

... selected for. In a commercial context, only simple measurements and recording procedures are possible, without interfering with the management practices and hence adding significantly to the costs of production. Hence the traits routinely recorded are easily and quickly measured. At slaughter, basic ...
Non-Mendelian Inheritance
Non-Mendelian Inheritance

... suggested a possible involvement of the nuclease C in mt– cpDNA destruction. But the hypothesis was rendered unlikely by the observation that the nuclease C activity was found to be present throughout the entire life cycle—in zygotes, mt+ and mt– vegetative cells, and gametes. Until now, the actual ...
PDF - Oxford Academic
PDF - Oxford Academic

... of the Lbc varieties. Available amino acid sequence data suggest that in this case the determined DNA sequence corresponds to Lbc^. However, this assignment is not conclusive since amino acid sequence analysis has not yet been completed on homogenous Lbc varieties (Whittaker, R.G., personal communic ...
Release Notes for Genomes Processed Using Complete Genomics
Release Notes for Genomes Processed Using Complete Genomics

letters
letters

... Next, we probed the functional relationship between EZH2 and DNA methyltransferases. As these proteins act as transcriptional repressors11,12,15, we investigated whether they can silence a common target gene. Recent work has identified several EZH2-target genes, including the MYT1 gene16. We first e ...
The eukaryotic genome: a system regulated at different hierarchical
The eukaryotic genome: a system regulated at different hierarchical

... 2002; Spellman and Rubin, 2002) pick up only clusters in which all genes are transcriptionally activated (or inactivated) simultaneously. Gene clusters that behave this way may be relatively rare. These data nevertheless indicate that eukaryotic genomes are functionally compartmentalized and that cl ...
Structural Variants in the Human Genome
Structural Variants in the Human Genome

... - Structural Variations are Common 12% of the genome (Redon et al. 2006) -Structural Variations are involved in phenotype variation and disease - Until recently most methods for detection were low resolution (>50 kb) ...
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics

...  Competent cells (勝任細胞) can pick up DNA from dead cells and incorporate it into genome by recombination (e.g. antibiotic resistance).  Transformed cell than passes genetic recombination to progeny.  Transformation works best when donor and recipient are related but they do not have to be. ...
Book 12 Chapter 34 - From The Mountain Prophecies
Book 12 Chapter 34 - From The Mountain Prophecies

... interconnected and all of them interlocked via tiny round locks! Yes, their “thinktanks” have worked around the clock to devise such horrendously evil schemes to steal the new DNA and to destroy the promises that it holds for all of humanity! But, even so, all of their schemes bear no fruit and to d ...
Genit 3
Genit 3

... d. with genes for the same characteristics at corresponding loci. One homologous chromosome is inherited from the organism's mother; the other from the organism's father.[1] They are usually not identical. Each chromosome in the pair contains genes for the same biological features, such as eye color ...
Lecture-Mic 623-Plasmids-Listeria - Home
Lecture-Mic 623-Plasmids-Listeria - Home

... Gene disruption by plasmid integration in Listeria monocytogenes: insertional inactivation of the listeriolysin determinant lisA. ...
A program for annotating and predicting the effects of single
A program for annotating and predicting the effects of single

... genome sequences. Once a genome is sequenced, the SnpEff program can be used to annotate and classify genetic polymorphisms based on their effects on annotated genes, such as synonymous or non-synonymous SNPs, start codon gains or losses, stop codon gains or losses; or based on their genomic locatio ...
Chapter 13 Mutation, DNA Repair, and Recombination
Chapter 13 Mutation, DNA Repair, and Recombination

... medium. Temperature-sensitive mutants will grow at one temperature but not at another. Suppressor-sensitive mutants are viable only when a second genetic factor, a suppressor, is present. © John Wiley & Sons, Inc. ...
Nov07-BalancersFinal
Nov07-BalancersFinal

Gene as the unit of genetic material - E
Gene as the unit of genetic material - E

... The part of the cell which occurs between the plasma membrane and nuclear envelope is known as the cytoplasm. It forms most essential part of the cell because it is seat of all biosynthetic and bio energetic functions. Most of the phenotypic characters are controlled by the genes present in the chro ...
Directed Evolution of Polymerases To Accept Nucleotides with
Directed Evolution of Polymerases To Accept Nucleotides with

... PCR using the high-fidelity Pfu UltraII (Stratagene) polymerase and 5′-CAGGAAGCAGCCATCAC-3′ as a primer. The products of the secondary PCR were then digested and cloned back into the original vector (a derivative of pASK43IBAplus) using the NcoI and EcoRI restriction sites. The ligated plasmids were ...
slides
slides

... Unigene contains several chimeric clusters The annotation of chimeric clusters is erratic ...
- Wiley Online Library
- Wiley Online Library

... (Fig. 1c) and six contained one or more large (> 50 amino acid) insertions; these same features were also observed in the corresponding P. capuana genomic sequences. One of the most extreme examples of genetic degeneration within the P. capuana carotenoid pathway is the transcript representing LCYB: ...
The Complete Mitochondrial DNA (mtDNA) of the Donkey and
The Complete Mitochondrial DNA (mtDNA) of the Donkey and

... the fragments were separated on a preparative agarose gel. The fragments were excised from the gel and extracted by electroelution. Fragments shorter than 4,000 nt (nucleotides) were ligated into M13 vectors, whereas larger fragments of XbaI, SpeI, and BamHI were redigested with enzymes producing co ...
Clinical application of High throughput sequencing (HTS) analysis
Clinical application of High throughput sequencing (HTS) analysis

Mechanisms and impact of genetic recombination in the evolution of
Mechanisms and impact of genetic recombination in the evolution of

... within each sliding window is compared to the average background SNP density for the whole genome to determine regions with significantly higher number of SNPs than expected by chance alone [24,63]. Such atypical regions are most likely to have been acquired through ...
of the number of ISs / Genome
of the number of ISs / Genome

A long-term demasculinization of X
A long-term demasculinization of X

... distribution of sex-biased genes, we expect male-biased ncRNAs to exhibit a chromosomal distribution similar to that observed for coding genes. In this report, we tested these hypotheses by experimentally identifying male-biased ncRNAs in D. melanogaster and analyzing their chromosomal distribution. ...
PDF
PDF

... the gene is likely to reside without experimental data such as full- ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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