Function of lanI in regulation of landomycin A biosynthesis in
... this big group of proteins resembles the OmpR transcriptional regulator of Escherichia coli phosphate regulon particularly in the region of DNA-binding domain formed by “winged” helix-turn-helix structure (Martinez-Hackert and Stock 1997; Harrison-McMonagle et al. 1999). Therefore, these transcripti ...
... this big group of proteins resembles the OmpR transcriptional regulator of Escherichia coli phosphate regulon particularly in the region of DNA-binding domain formed by “winged” helix-turn-helix structure (Martinez-Hackert and Stock 1997; Harrison-McMonagle et al. 1999). Therefore, these transcripti ...
Determination of primary structure
... BIOINFOMATICAL We just went through the CHEMICAL and PHYSICAL. The BIOINFORMATICAL method requires information from chemical or physical, but only a limited amount of sequence. Example: a sequence of 6 AA is only possible as one of 206 possible hexa-peptide sequences (1 of 64 x 106). There are no mo ...
... BIOINFOMATICAL We just went through the CHEMICAL and PHYSICAL. The BIOINFORMATICAL method requires information from chemical or physical, but only a limited amount of sequence. Example: a sequence of 6 AA is only possible as one of 206 possible hexa-peptide sequences (1 of 64 x 106). There are no mo ...
A-10484A SNPs. Mutations and DNA Sequence
... the sample plate layout. Before starting the run make sure the capillaries are preheated to 50˚C immediately prior to starting the run. This ensures proper separation for the first row of samples. Preheating is not required for subsequent rows within the same run. Observe the raw data for the first ...
... the sample plate layout. Before starting the run make sure the capillaries are preheated to 50˚C immediately prior to starting the run. This ensures proper separation for the first row of samples. Preheating is not required for subsequent rows within the same run. Observe the raw data for the first ...
Colicins produced by the Escherichia fergusonii strains closely
... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
Separation of DNA Restriction Fragments by Ion
... plays a central role in DNA research. However, when used for preparative purposes electrophoresis has two particularly serious drawbacks: (A) Extraction of the purified material out of the gel is tedious and difficult to accomplish with high yields (1). (B) The purified DNA is often contaminated wit ...
... plays a central role in DNA research. However, when used for preparative purposes electrophoresis has two particularly serious drawbacks: (A) Extraction of the purified material out of the gel is tedious and difficult to accomplish with high yields (1). (B) The purified DNA is often contaminated wit ...
Evaluation of the phylogenetic position of the planctomycete
... whose sequences can be concatenated to infer phylogeny. In addition, it has been demonstrated recently that the extent to which LGT affects typical phylogenetic protein markers might have been overestimated (Daubin et al., 2003). Concatenated ribosomal protein sequences have been applied successfull ...
... whose sequences can be concatenated to infer phylogeny. In addition, it has been demonstrated recently that the extent to which LGT affects typical phylogenetic protein markers might have been overestimated (Daubin et al., 2003). Concatenated ribosomal protein sequences have been applied successfull ...
THE EFFECT OF R-FACTOR CARRIAGE ON THE SURVIVAL O F
... a progressively increasing population of resistant organisms (Finland, 1972). Since the discovery of antibiotic-resistance transfer (R) factors (Watanabe, 1963) a high proportion of antibiotic resistance in the Enterobacteriaceae and pseudomonads has been shown to be mediated by these plasmids. Howe ...
... a progressively increasing population of resistant organisms (Finland, 1972). Since the discovery of antibiotic-resistance transfer (R) factors (Watanabe, 1963) a high proportion of antibiotic resistance in the Enterobacteriaceae and pseudomonads has been shown to be mediated by these plasmids. Howe ...
Diploidization of meiosis in autotetraploids
... chromosomes, and trivalents, which are composed of three. If they are not resolved, multivalents can persist through metaphase I, leading to high rates of aneuploid gamete formation and sterility [14]••. In order to understand how multivalents form, the concept of the autonomous pairing site (APS) i ...
... chromosomes, and trivalents, which are composed of three. If they are not resolved, multivalents can persist through metaphase I, leading to high rates of aneuploid gamete formation and sterility [14]••. In order to understand how multivalents form, the concept of the autonomous pairing site (APS) i ...
The viriosphere, diversity, and genetic exchange within phage
... of the cell (see [34] for a review of this system in Synechococcus). At least one of these genes, as well as a varying number of other related photosynthesis genes, have been reported in cyanophages that infect Synechococcus [35] and/or Prochlorococcus [36]. They have also been identified in BA ...
... of the cell (see [34] for a review of this system in Synechococcus). At least one of these genes, as well as a varying number of other related photosynthesis genes, have been reported in cyanophages that infect Synechococcus [35] and/or Prochlorococcus [36]. They have also been identified in BA ...
Repetitive complete hydatidiform mole can be biparental in origin
... pregnancy, is associated with the presence of two paternal genomes and thus involves imprinted genes, that is genes which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increa ...
... pregnancy, is associated with the presence of two paternal genomes and thus involves imprinted genes, that is genes which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increa ...
A phenotype-based screen for embryonic lethal mutations in the mouse
... marker is the marker that showed no recombination with the mutation (except for 105, see*) [Mouse Genome Database (MGD) map position]. No. nonrecombinants, number of independent meiotic products in which the chromosomes carried both the mutation and the closest marker, including data from both heter ...
... marker is the marker that showed no recombination with the mutation (except for 105, see*) [Mouse Genome Database (MGD) map position]. No. nonrecombinants, number of independent meiotic products in which the chromosomes carried both the mutation and the closest marker, including data from both heter ...
Genetics - Michael
... this chapter, as well as an introduction to DNA replication. The study of DNA replication has special importance for geneticists. Once scientists determined the process by which a cell copies its DNA it became possible to mimic this procedure in a test tube. The end result was the invention of the p ...
... this chapter, as well as an introduction to DNA replication. The study of DNA replication has special importance for geneticists. Once scientists determined the process by which a cell copies its DNA it became possible to mimic this procedure in a test tube. The end result was the invention of the p ...
Special Feature —Manipulating Genes to Understand
... advances in our understanding of the mechanisms of gene expression and regulation and the development of animal models of human diseases are but two examples of how this technology has affected medical science. Transgenic animals are defined as animals in which a segment of DNA has been physically i ...
... advances in our understanding of the mechanisms of gene expression and regulation and the development of animal models of human diseases are but two examples of how this technology has affected medical science. Transgenic animals are defined as animals in which a segment of DNA has been physically i ...
Molecular Classification of Infectious Laryngotracheitis Virus from
... vaccinated twice with CEO and/or TCO vaccines. Broilers are vaccinated only in cases of outbreaks with the CEO vaccines. The increased frequency of outbreaks in broilers has been associated with dense poultry populations, mixing of different types of poultry in the same geographical area, shorter do ...
... vaccinated twice with CEO and/or TCO vaccines. Broilers are vaccinated only in cases of outbreaks with the CEO vaccines. The increased frequency of outbreaks in broilers has been associated with dense poultry populations, mixing of different types of poultry in the same geographical area, shorter do ...
A survey of denitrifying Azospirillum brasilense in two contrasted
... The nir test was used also to calculate the most probable number (MPN) of denitrifying Azospirillum from root samples of each locality of the two regions considered in this work, following the prescriptions of Döbereiner et al. (1995) and by using the McCrady table for 5 replicates. PCR amplificatio ...
... The nir test was used also to calculate the most probable number (MPN) of denitrifying Azospirillum from root samples of each locality of the two regions considered in this work, following the prescriptions of Döbereiner et al. (1995) and by using the McCrady table for 5 replicates. PCR amplificatio ...
rational selection of pcr-based platforms for pharmacogenomic testing
... development of neoplastic diseases and individual variations in responses to specific drugs. Costand time-effective technologies able to accurately identify genetic polymorphisms will dramatically affect routine diagnostics processes and future therapeutic developments. However, such methods need to ...
... development of neoplastic diseases and individual variations in responses to specific drugs. Costand time-effective technologies able to accurately identify genetic polymorphisms will dramatically affect routine diagnostics processes and future therapeutic developments. However, such methods need to ...
Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... alleles, probably deriving from specific individual mutations that are characterized by nonpigmentation of the leaves (Bartley, 2005). A similar effect was observed in F2 progenies, resulting from self-fertilization of F1 plants from Pa 121 x SIC 802 and Pa 121 x Pa 169 (Yamada et al., 1982). One fo ...
... alleles, probably deriving from specific individual mutations that are characterized by nonpigmentation of the leaves (Bartley, 2005). A similar effect was observed in F2 progenies, resulting from self-fertilization of F1 plants from Pa 121 x SIC 802 and Pa 121 x Pa 169 (Yamada et al., 1982). One fo ...
Genetic Markers for Sex Identification in Forensic DNA Analysis
... [7]. The protein coding regions, exons 2-6, of the gene are highly conserved [6,7]. The PCR products of AMELX and AMELY can be discriminated from one another using primers flanking a 6 bp deletion in the third intron of AMELX that is not present in AMELY [5,8-10]. The most commonly used amelogenin p ...
... [7]. The protein coding regions, exons 2-6, of the gene are highly conserved [6,7]. The PCR products of AMELX and AMELY can be discriminated from one another using primers flanking a 6 bp deletion in the third intron of AMELX that is not present in AMELY [5,8-10]. The most commonly used amelogenin p ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.