• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Review A model for chromosome structure during the mitotic
Review A model for chromosome structure during the mitotic

... matrix strands. This orientation is maintained into prophase when the nuclear matrix strand is converted into two closely associated sister chromatid cores with sister DNA loops extending in opposite directions. We propose that chromatid cores are contractile and show, using a physical model, that c ...
Relatedness of penicillin-resistant Streptococcus
Relatedness of penicillin-resistant Streptococcus

Extending FISH analysis of Paediatric Tumours
Extending FISH analysis of Paediatric Tumours

R4, a non-LTR retrotransposon specific to the
R4, a non-LTR retrotransposon specific to the

... that R4 elements were present in C.elegans, as variant rDNA units had been previously characterized from this species and no insertion elements were found (26). Only the PCR amplifications with P.equorum and H.contortus DNA gave rise to an appropriately sized band on agarose gels (1.5-1.8 kb). These ...
ppt - pedagogix
ppt - pedagogix

... The process can be iterated several times • collect sequences > build a profile -> collect sequences -> build a profile ... ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter

EFFECTS OF ACIDS ON FOREST TREES AS MEASURED BY
EFFECTS OF ACIDS ON FOREST TREES AS MEASURED BY

... crossings of P. abies were i n v e s t i g a t e d . Buffering capacity was measured a s consumption of H C 1 i n u 1 f o r a pH-change of one u n i t . Needle homogenates from 23 f a m i l i e s of 7 p a r e n t t r e e s , each family c o n s i s t i n g of 24 t r e e s , p l a n t e d i n a t r i ...
IOSR Journal of Pharmacy and Biological Sciences (IOSRJPBS)
IOSR Journal of Pharmacy and Biological Sciences (IOSRJPBS)

... physiological characteristics. Molecular techniques involving ribosomal RNA sequence analysis are commonly used to investigate evolutionary relationships within different genera of bacteria. In a routine antibiotic screening strategy, the strains producing interesting metabolites in screening are us ...
Methods of Human Heredity Study
Methods of Human Heredity Study

... Chromomeres are regular features of all prophase chromosomes, larger enough to reveal them, but their number, size; distribution and arrangement are specific for a particular species at a particular stage of development. Chromocentres are heterochromatic regions of varying size, which occur near the ...
Computer-Aided DNA Base Calling from Forward and Reverse
Computer-Aided DNA Base Calling from Forward and Reverse

A Pneumocystis carinii multi-gene family with
A Pneumocystis carinii multi-gene family with

... a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave positive hybridization signals compared to the number when the ...
Accepted Version - CSIRO Research Publications Repository
Accepted Version - CSIRO Research Publications Repository

Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of

15.13 Spm elements influence gene expression
15.13 Spm elements influence gene expression

... Insertion sequences are simple transposition modules Composite transposons have IS modules Transposition occurs by both replicative and nonreplicative mechanisms 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a ...
0 - Microbiology
0 - Microbiology

... The relationship between genetic constitution and biochemical function had emerged from the combination of biochemical and genetical analysis that Beadle & Tatum (1941) had so successfully employed with Neurospora crassa. In order to apply similar techniques to bacteria, Lederberg & Tatum (1946a, b) ...
Figure 15.6 Nonreplicative transposition allows a transposon to
Figure 15.6 Nonreplicative transposition allows a transposon to

... Insertion sequences are simple transposition modules Composite transposons have IS modules Transposition occurs by both replicative and nonreplicative mechanisms 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a ...
Robust Prediction of Expression Differences among Human
Robust Prediction of Expression Differences among Human

... statistically significant associations of single SNPs, they do not consider multiple-SNP models, where combinations of SNPs and SNPs with small effects could play a role in generating the predictive model. One work attempted to devise a multiple-SNP predictive model using a cross validation scheme, ...
The Evolution of CONSTANS-Like Gene Families
The Evolution of CONSTANS-Like Gene Families

... the CO transcript is rare in Arabidopsis (SuárezLópez et al., 2001), and orthologs might behave similarly in barley, making them difficult to isolate from cDNA libraries. In cases where Morex BAC clones were used, the corresponding gene from Igri was amplified by PCR from genomic DNA and sequenced ...
Electrophoresis Revised
Electrophoresis Revised

... an air-tight seal. Having said this, do NOT jam the tips on so hard that they are hard to get off. The tips used for the 1000μL pipettes are usually blue. The volume to be taken up is set by turning the plunger on the top of the micropipette and reading the numerical settings displayed. A setting of ...
Trawling DNA Databases for Partial Matches: What is the FBI Afraid
Trawling DNA Databases for Partial Matches: What is the FBI Afraid

... CORNELL JOURNAL OF LAW AND PUBLIC POLICY ...
Transcriptional analysis of the gene for glutamine synthetase II and
Transcriptional analysis of the gene for glutamine synthetase II and

... glnII) activity detectable in crude extracts from Streptomyces coelicolor is low compared to the activity of glutamine synthetase I (GSI, encoded by glnA) and to that of GSII from S. viridochromogenes. We have identi®ed and sequenced a 3.9-kb BglII-BamHI fragment carrying the glutamine synthetase II ...
BioACTS Quarter THREE
BioACTS Quarter THREE

... dominant=C; recessive=c): If a father has normal vision but a mother is heterozygous for the trait, what is the probability that a son is born who is red-green color blind? PROVE IT WITH A PUNNETT SQUARE!!!! ...
Two supernumerary marker chromosomes
Two supernumerary marker chromosomes

Horizontal transfer generates genetic variation in an asexual
Horizontal transfer generates genetic variation in an asexual

Maternal uniparental disomyof chromosome 13
Maternal uniparental disomyof chromosome 13

... reported involving all or part of chromosomes homozygous despite all being highly (>75%) 6,7,11,14,15,16,21,22, and the XY pair.'4 polymorphic.'2 Such a chromosome could have These investigations are of value firstly for been formed by a translocation involving the determining which chromosomes cont ...
< 1 ... 23 24 25 26 27 28 29 30 31 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report