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Name _______________________________________________ Date ______________ Observing Onion Cells.2015 P2 P3 P4 P6 Activity: Observing a Non-green Plant Cell (Onion Cells) Goal: To observe, identify and describe the basic organelles of a non-green plant cell. Background Information: All living things are made up of small units called cells. That is, cells are the basic building blocks of living things just as atoms are the basic building blocks of matter. Each cell contains living material surrounded by a border, or barrier that separates the cell from its environment. Some living things contain only a single cell. One-celled organisms are prokaryotes or prokaryotic. Their genetic material is not surrounded by a membrane. Bacteria are prokaryotes. Other organisms ranging from single-celled protists to multicellular plants and animals are eukaryotes or eukaryotic. Their genetic material is membrane-bound. Nevertheless, every single cell whether in a unicellular or multicellular organism can perform all the functions necessary for life. Unicellular organisms may contain hundreds, thousands, or even trillions of cells. Materials: onion slices, iodine stain, forceps, blank slides, coverslips, scissors, toothpicks Procedure: 1. What I Know: Write two sentences about what you already know or think you know about plant cells. 2. Place a drop of water on a slide. 3. Peel away a layer of onion slice and then peel off a very thin strip of onion membrane from the inside of the layer. 4. Place the membrane on the water droplet on your slide. Try not to wrinkle the membrane. 5. Add a drop of iodine to the slide and add a coverslip. 6. Observe your slide at 40X and then at 100X. P7 7. What I Observed: Make two illustrations of what you observed one at 40 X and the other at 100 X. In the higher power drawing, label the nucleus, the cell membrane, the cell wall, and the cytoplasm. Use proper biological drawing techniques. Write one caption describing your observations. 40X Onion Cells 100X Onion Cells Caption: Write one caption describing your observations. 8. Questions: Answer the following in complete sentences. a. The cells of the onion are colorless, yet the onion is part of a green plant. Why do you think the onion cells you observed are not green? b. Describe the structure and the function of the cell wall in plants. 9. What I Wonder: Pose a “why” or “how” question that you still may have concerning this topic. 10. What I Learned: CER (Claim, Evidence, Reasoning) Writing Prompts: Use the following prompts to help you construct your lab conclusion, What I Learned. Goal: State the goal of this lesson. The goal of this lesson is… Claim: What did you learn from the activity that satisfies the goal of this lesson? This is an I Learned statement. I learned that… Evidence: How can you prove from your observations (data) that you learned what you claim? This can be a general statement that you will explain in detail in your explanation. I know this because…. Explanation/Reasoning: Include the scientific principles that connect the evidence and claim. (What’s the science that helps explain your claim and evidence. The information can be from what you learned in our class discussions, the textbook, or other resources that will help support your claim or enhance your explanation. Conclusion: Summarize the claim of the lesson (reword the claim) and include a connection to self, text, or world. Name ____________________________________Date __________________ Lab Activity: Observing Onion Cells What I Learned: Write a summary of what you learned by completing this activity. The summary should state a claim that is related to the goal of the lesson, provides evidence from your observations, offers a scientific explanation (reasoning) that links your claim to the evidence, and includes a conclusion.