Download BTEC Bowl Questions

TEAM ALPHA BTEC BOWL QUESTIONS:
Chapter 1:
Michelle Quesnel:
Q: A DNA microarray contains:
A: A. double-stranded DNA molecules
B. single-stranded DNA molecules
C. pharmacogenomics
D. all of the above
(pg 15 and glossary pg G-6)
Suzanne Visser:
Q: How are recombinant proteins produced?
A: By a gene cloning technique involved in transferring a gene of interest into bacteria or
mammalian cells that are grown in cell cultures with the necessary nutrients for cell growth.
These are mass produced in bioreactors. (pg 6-7)
Suzanne Visser:
Q: What are two primary goals of the Human Genome Project and in what year did it begin?
A: 1990. The goals were to identify all the genes in the DNA of a human cell and to map the
location of each gene to the 24 chromosomes. (pg 4)
Suzanne Visser:
Q: Name a everyday life use of biotechnology with an example.
A: Genetically modified foods (GM), such as corn. (pg 2)
Hans Cooper:
Q: Applying computer science to the study of DNA and protein data is a new field called:
A: A. proteonomics
B. bioreactor technology
C. bioinformatics (p. 6)
D. recombinant studies
E. none of the above
Hans Cooper:
Q: The use of plants as sources of pharmaceutical products is an application of agricultural
biotechnology called:
A: A. agricultural recombination
B. molecular pharming (p. 9)
C. farming enhancement
D. drug farming
E. none of the above
Chris Rosario:
Q: In biomanufacturing operations, this process is used to grow large amounts of products from
cell cultures.
A: A. molecular pharming
B. pharmacogenomics
C. scale-up (p. 22)
D. recombinant technology
Chapter 2:
Michelle Quesnel:
Q: Stretches of DNA that do not contain protein coding information are called
A: Introns (pg 41)
.
Suzanne Visser:
Q: What is the technique used to introduce genes into bacteria from a forgein source? Explain
the experiment Griffith did.
A: Transformation. The experiment showed evidence that genetic material from dead S cells
with protein coat changed the living R cells without a protein coat into S cells. These bacterial
cells uptook the DNA from the S cells and made the R cells virulent. (pg 31)
Suzanne Visser:
Q: Explain what model DNA follows during replication?
A: Semiconservative model. Each strand in the double helix of the original DNA molecule serves
as a template for the daughter strands, that are made through complimentary base pairing by
DNA polymersase.Two new double helises are formed with one parents strand and one
daughter strand. Then each is seperated again, and used as a template again to synthesis
helises exponentially (pg 37).
Hans Cooper:
Q: Transformation is the process of:
A: A. changing protein sequences in cells
B. moving existing DNA sequences within a genome
C. improving the RNA sequences of plants
D. the uptake of DNA by bacterial cells (p.31)
E. All of the above
Hans Cooper:
Q: Ribosomes consist of:
A: A. aggregates of rRNA
B. proteins that form structures called subunits
C. sources of DNA
D. (A) and (B) are both correct (p. 44)
E. none of the above
Chris Rosario:
Q: In gamete formation, parent cells can divide into as many as four daughter cells in a process
known as:
A: A. mitosis
B. meiosis (p. 37)
C. sister chromatids
D. alternative splicing
Chapter 3:
Nathan McMichen:
Q: What step of PCR allows for the addition of primers, and at what ends of the template
strands?
A: Step 2 annealing. The primers attach to the 3' ends of the template strands.
Marcel Munganga:
Q: compare to the upstream phase processing uses in the production of gfp proteins; the
downstream processing phase includes:
A: A. The actual expression of the protein in the cell.
B. Separation of the protein from other parts of the cell, and isolation from other protein.
C. Verification of protein purity and its functional abilities.
D. Both choices a and b are correct.
E. Both choices b and c are correct.
Hans Cooper:
Q: agarose gel electrophoresis is:
A: A. a common technique used to separate and visualize DNA fragments. (p. 75)
B. part of the process of creating a restriction map.
C. uses marker channels that serve as a ladder of fragments of known sizes.
D. is used to compare sizes of fragments.
E. all the above. (p. 75-77)
Hans Cooper:
Q: Stone age genomics are:
A: A. an ancient technique for analyzing DNA data
B. analysis of ancient DNA samples (p.93)
C. an exciting program at the Smithsonian Museum
D. the original method of protein analysis
E. none of the above
Chapter 4:
Nathan McMichen:
Q: What is the process called in which proteins are separated according to their repulsion of
water through a column of beads coated with hydrophobic molecules?
A: HIC (Hydrophobic interaction chromatography).
Marcel Munganga:
Q: For the expression and purification of the GFP (laboratory experiment #4); Hydrophobic
interaction chromatography (HIG) is a technique that:
A: A. Uses gel beads as a filtering system for protein purification.
B. Relies on electrostatic charge (static cling) to bind the protein to the gel beads in the
column.
C. Relies on the ability of most proteins to bind specifically and reversibly to uniquely
shaped compounds called ligands.
D. Is only used in the later stages of the purification process.
E. Is a technique in which the proteins are sorted on the basis of their repulsion of water.
Michael Singh:
Q: Infectious proteins that attract normal cell proteins and induce changes in their structure by
causing faulty protein folding is called:
A: prion (pg.105.)
Hans Cooper:
Q: The purpose(s) of adding enzymes to detergents is/are:
A: A. to help detergents do a better job of cleaning.
B. to make them more biodegradeable.
C. to modify the DNA of the proteins in detergents.
D. both (A) and (B) are correct. (p. 101)
E. (A), (B) and (C) are all correct.
Hans Cooper:
Q: The two major phases involved in producing proteins are called:
A: A. early and late production phases.
B. primary and secondary phases.
C. first and second processing phases.
D. Upstream and downstream processing. (p.107)
E. none of the above.
Chapter 5:
Phillip Kuether:
Q: Bacitracin, Erythromycin, Neomycin, Streptomycin, and Tetracycline are all antibiotics
identified from bacteria.
A: (True / False)
Denise Masters:
Q: Electroporation is the process by which DNA is introduced into bacterial cells through a brief
electrical shock; which does not kill most of the cells?
A: True / False (pg 124)
Mathew Aderholt:
Q: What type of vaccine is prepared by killing the pathogen and contains the "dead" virus?
A: A. Attenuated Vaccine
B. Inactivated Vaccine (pg 137)
C. Subunit Vaccine
D. HPV Vaccine
Ayamo Oben:
Q: Lassa fever is caused by:
A: A. bacteria
B. Fungal infection
C. virus
D. protozoa
Ayamo Oben:
Q: What is the disadvantage of using lassa virus as a bioterrorism weapon?
A: A. causes diseases
B. too expensive
C. long incubation
D. not easily available
Ayamo Oben:
Q: True or False? Today plant biotechnology encompasses two major areas, plant tissue
cultures and genetic recombination.
A: False- Plant tissue cultures and genetic engineering.
Hans Cooper:
Q: Bioprospecting consists of:
A: A. searching for interesting DNA sequences.
B. trying to identify dangerous microbes.
C. searching for potentially valuable microorganisms. (p.123)
D. trying to find rare species of plants so they can be protected from extinction.
E. none of the above.
Hans Cooper:
Q: Antibiotics:
A: A. can be produced by other microbes.
B. inhibit the growth of microbes.
C. form IGg, a bacteriocidal agent.
D. both (A) and (B) are correct. (p. 131)
E. (A), (B) and (C) are all correct.
Chapter 6:
Phillip Kuether:
Q: The transformed genes from chloroplast engineering could be spread to multiple plants
through the pollen.
A: (True / False, chloroplast DNA is separate)
Denise Masters:
Q: Who regulates the food on the market?
A: A. CDC
B. AAA
C. FDA (p. 169)
D. AEA
Mathew Aderholt:
___________ uses small discs cut from mature plants that are cultured and stimulated to
produce an engineered plant.
A. Antisense technology
B. Chloroplast engineering
C. Leaf Fragment technique (pg 158)
D. Protoplast fusion
Hans Cooper:
Q: Polygalacturonase is:
A: A. an enzyme that acts as an insecticide in corn.
B. an enzyme that that digests pectin in the wall of a plant. (p.159)
C. a GM enzyme used to enhance storage of corn
D. part of the Krebs cycle
E. none of the above
Hans Cooper:
Q: Glyphosphate is:
A: A. an enzyme that acts as an insecticide in corn.
B. an herbicide that works by blocking an enzyme needed in photosythesis (p.163)
C. a GM enzyme used to enhance storage of corn
D. part of the Krebs cycle
E. none of the above
Chapter 7:
Hans Cooper:
Q: Which of the following are commonly used in genetic research for animals:
A: A. Zebrafish.
B. Mice.
C. Rats.
D. Tomatoes.
E. (A), (B) and (C) are all correct. (p. 174)
Hans Cooper:
Q: Enucleation consists of:
A: A. preparing an egg for cloning.
B. gently suctioning the DNA out of the nucleus
C. recombining the DNA from one nucleus with another nucleus.
D. both (A) and (B) are correct. (p. 177)
E. (A), (B) and (C) are all correct.
Chris Rosario:
Q: The process in which modified DNA is added to embryonic stem cells so that it recombines
and deletes or replaces part of the genetic instructions of the cell is known as:
A: homologous recombination (p. 184)
Chris Rosario:
Q: The three R’s of animal research include:
A: A. reduce, reuse, recycle
B. replace, recycle, remediate
C. refine, recombine, reuse
D. reduce, replace, refine (p. 176)
Chapter 9:
Hans Cooper:
Q: Anaerobic biodegradation relies on:
A: A. iron (Fe+3), sulfate (SO4-2), and nitrate (NO3-).
B. oxidization of pollutants.
C. anaerobic bacteria.
D. none of the above.
E. (A), (B) and (C) are all correct. (p. 213)
Hans Cooper:
Q: Bioaugmentation:
A: A. involves adding bacteria to the contaminated environment.
B. is intended to assist indigenous microbes with biodegradative processes.
C. is a bioremediation approach involving phosphorus and nitrogen to grass.
D. both (A) and (B) are correct. (p. 177)
E. none of the above.