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Plant kingdom http://www.istockphoto.com/file_thumbview_approve/6143246/2 /istockphoto_6143246-chloroplasts.jpg Plant kingdom http://www.childrensmicroscopes.com/images/specimennew/022aab01022abc40x-onion-epidermis.jpg Note the thick barrier between cells in all of these images of plant cells. It is the cell wall. Source for all photographs of cells belowe http://www.ndpteachers.org/perit/biology_image_gallery1.htm Plant root tip Plant leaf Water conducting tissue of bamboo, a plant plantanatomy.org/biological-photographs.html Mesophyll cells (photosynthetic cells) of a plant leaf plant wanimal— note (e.g., in the circled area) that the intersection of cells is sometimes difficult to distinquish: cell wall is not present. _____ 40 µm fish blastocyst (early embryo), stained for DNA http://slohs.slcusd.org/pages/teachers/bsmith/ZZ%20%20Folder/Ron%27 s%20Biology%20Web%20Page/Biology/Cells/onion3.jpg Mammalian squamous epithelial cells ________ 70 µm Amoeba -protist ____________________________200 microns 1 celled, no cell wall nucleus (animal like protists have no cell walls but plant like protists usually do have cell walls); note vacuoles (uneven granule like appearance) in the cytoplasm, containing food that the amoeba ingested by phagocytosis. Paramecium protist 1 celled, no cell wall nucleus (animal like protists have no cell walls but plant like protists usually do have cell walls); note vacuoles (uneven granule like appearance) in the cytoplasm, containing food that the amoeba ingested by phagocytosis. ____________________________100 microns 1 celled, no cell wall nucleus (animal like protists have no cell walls rat seminal tubules in presence of estrogen, bar = 50 microns human kidney cells http://www.ehponline.org/members/1999/107p397-405fisher/fisher-full.html http://www.nature.com/ki/journal/v71/n5/thumbs/5002030f2th.jpg note that it is not easy to distinguish boundaries between many of the cells (look at cells comprising the tube), and nuclei are found in the cells. Also, cells are between about 10 and 30 um Note that as the entire specimen is scanned, areas with different appearing cells are clearly evident. This shows that the organism is multicellular and has specialized cell types. www.siumed.edu/~dking2/erg/GI118b.htm cross section of intestine in animal _______ 50 um Chinese hamster ovary cells micro.magnet.fsu.edu/.../chocells.html Note that these cells overlap are stacked haphazardly—an indication that these cells have been “transformed” and are on the way to becoming tumor forming cells (they are not obeying rules to stop dividing when cells come into contact with the membranes of adjacent cells). The cells are also very flat and irregular in appearance versus the other cells of the same type (these are clones of a single cell), another indication that the cell is abnormal. Note that different regions have very distinct cell stains and cell morphology (appearance), showing that the organism is multicellular and specialized with regard to its cell types. The tiny purple spots above GI118 in the website address are nuclei in many adjacent cells. ___ 100 µm The entire specimen is well over a mm in size—definitely visible to the naked eye, so this is certainly MANY cells (multicellular). Carolina biological, coccal (round shape) Carolina biological, http://www.hometrainingtools.com/pop_multi_view.asp?pn=MSBACTERA&pname=Bacteria+slide,+three+types,+smear note the spiral shape (spirilla) i ___10 microns Bacilli (rod shape) surrounded by eukaryotic cells— note that the eukaryotic nuclei are alone much larger than the bacteria. Also, note the difficulty in determining where the “edges” of the eukaryotic cells are (hard to see the separation of the cells housing adjacent nuclei, the dark purple spots). Note the nucleoli in each nucleus (the darker purple spot in the nucleus). Into what kingdom do you think the host cells ought be classified? How do you recognized all of these as cells? The cells are smaller than 5 microns, whereas 7 microns is the lower size limit for a nucleus. Also, the 3 shapes are revealing. www.gadgetscience.com/tag/science-project/ Note that the cells surrounding the bacteria are eukaryotic (clusters of 4 or 5 cells, each with a nucleus) and are http://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio %20102/Bio%20102%20Laboratory/Fungi/Fungi.htm High magnification staphylococcus bacteria http://phil.cdc.gov/PHIL_Images/2296/2296_lores.jpg Images below are fungi plantclinic.cornell.edu/.../brownpatch.htm Note the obvious barriers that mark the outer limits of the cells that form the matted, string like body called a mycelium (see the dark brown rim above and the dark purple rim in the photograph on the right). Also note that there are few different types of cells, just the hyphae (string like cells connected together to form the mucelium) In the photograph on the right, also note the thick walled spores at the timps of some hyphae. Nuclei are not stained in these cells, but they would be apparent if stained or if the section was viewed at different depths. http://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio %20102/Bio%20102%20Laboratory/Fungi/Fungi.htm ____150 microns