Download Chapter 8 Cellular Respiration Dr. Harold Kay Njemanze 8.1

Chapter 8 Cellular Respiration
Dr. Harold Kay Njemanze
8.1 Cellular Respiration
1. Cellular respiration involves various metabolic pathways that break down
carbohydrates and other metabolites with the resultant buildup of ATP.
2. Cellular respiration consumes oxygen and produces CO2; because oxygen is
required, cellular respiration is aerobic.
3. Cellular respiration usually involves the complete breakdown of glucose into CO2
and H2O.
4. The net equation for glucose breakdown is: C6H12O6 + 6 O2 = 6 CO2 + 6 H2O +
energy
5. Cellular respiration releases energy, it is therefore exergonic.
6. Electrons are removed from substrates and received by oxygen, which combines
with H+ to become water.
7. Glucose is oxidized and O2 is reduced.
8. The reactions of cellular respiration allow energy in glucose to be released
slowly; therefore ATP is produced gradually.
9. The breakdown of glucose yields synthesis of 38net ATP and 40 total ATP.
A. NAD+ and FAD
1. Each metabolic reaction in cellular respiration is catalyzed by a specific enzyme.
2. As a metabolite is oxidized, NAD+ (nicotinamide adenine dinucleotide) accepts
two electrons and a hydrogen ion (H+); this results in NADH + H+.
3. Electrons received by NAD+ and FAD are high-energy electrons and are usually
carried to the electron transport chain.
4. NAD+ is a coenzyme of oxidation-reduction since it both accepts and gives up
electrons; thus, NAD+ is sometimes called a redox coenzyme
5. Only a small amount of NAD+ is needed in cells because each NAD+ molecule is
used repeatedly.
6. FAD coenzyme of oxidation-reduction can replace NAD+; FAD accepts two
electrons and two hydrogen ions to become FADH2.
B. Phases of Cellular Respiration
1. Cellular respiration includes THREE phases:
A. Glycolysis is the breakdown of glucose in the cytoplasm into two molecules
of pyruvate.
1) Enough energy is released for an immediate yield of two ATP.
2) Glycolysis takes place outside the mitochondria and does not utilize
oxygen; it is therefore an anaerobic process.
Sub a.
In the preparatory (prep) reaction, pyruvate enters a mitochondrion
and is oxidized to a two-carbon acetyl group and CO2 is removed; this
reaction occurs twice per glucose molecule.
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B. The citric acid cycle:
1) occurs in the matrix of the mitochondrion and produces NADH and
FADH2;
2) is a series of reactions that gives off CO2 and produces one ATP;
3) turns twice because two acetyl-CoA molecules enter the cycle per
glucose molecule;
4) produces two immediate ATP molecules per glucose molecule.
C. The electron transport chain:
1) is a series of carriers in the inner mitochondrial membrane that accept
electrons from glucose--electrons are passed from carrier to carrier
until received by oxygen;
2) passes electrons from higher to lower energy states, allowing energy to
be released and stored for ATP production;
8.2 Outside the Mitochondria: Glycolysis
1. Glycolysis occurs in the cytoplasm outside the mitochondria.
2. Glycolysis is the breakdown of glucose into two pyruvate molecules.
3. Glycolysis is universally found in organisms; therefore, it likely evolved before
the citric acid cycle and electron transport chain.
4. Glycolosis can be divided into the energy-investment steps where ATP is used to
“jump start” glycolosis, and the energy-harvesting steps, where more ATP is
made than used.
A. Energy-Investment Steps
1. Glycolysis begins with the activation of glucose with two ATP; the glucose splits
into two C3 molecules known as G3P, each of which carries a phosphate group.
B. Energy-Yielding Steps
1. Oxidation of G3P occurs by removal of electrons and hydrogen ions.
2. Two electrons and one hydrogen ion are accepted by NAD+, resulting in two
NADH; later, when the NADH molecules pass two electrons to the electron
transport chain, they become NAD+ again.
3. The oxidation of G3P and subsequent substrates results in four high-energy
phosphate groups, which are used to synthesize four ATP molecules; this
process is called substrate-level phosphorylation.
4. Two of four ATP molecules produced are required to replace two ATP molecules
used in the initial phosphorylation of glucose; therefore there is a net gain of
two ATP from glycolysis.
5. Pyruvate enters a mitochondrion (if oxygen is available) and cellular respiration
ensues.
6. If oxygen is not available, fermentation occurs and pyruvate undergoes
reduction.
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8.3 Fermentation
1. Fermentation is an anaerobic (i.e., occurs in the absence of oxygen) process
which consists of
glycolysis
plus reduction of pyruvate to either lactate or to alcohol and CO2 (depending on
the organism).
2. Animal cell fermentation results in lactate.
3. Bacteria can produce an organic acid like lactate, or an alcohol and CO2.
4. Yeasts produce ethyl alcohol and CO2.
5. NADH passes its electrons to pyruvate instead of to an electron transport
chain; NAD+ is then free to return and pick up more electrons during earlier
reactions of glycolysis.
A. Advantages and Disadvantages of Fermentation
1. Despite a low yield of two ATP molecules, fermentation provides a quick burst
of ATP energy for muscular activity.
2. Fermentation products are toxic to cells.
a. When blood cannot remove all lactate from muscles, lactate changes pH and
causes muscles to fatigue.
b. The individual is in oxygen debt because oxygen is needed to restore ATP
levels and rid the body of lactate.
c. Recovery occurs after lactate is sent to the liver where it is converted into
pyruvate; some pyruvate is then respired or converted back into glucose.
B. Efficiency of Fermentation
1. Two ATP produced per glucose molecule during fermentation is equivalent to
14.6 kcal.
2. Complete glucose breakdown to CO2 and H2O during cellular respiration
represents a potential yield of 686 kcal per molecule.
3. Efficiency of fermentation is 14.6/686 or about 2.1%, far less efficient than
complete breakdown of glucose.
C. Fermentation Helps Produce Numerous Food Products (Science Focus box)
1. Yeast Fermentation
a. Baker’s yeast, Saccharomyces cerevisiae, is added to bread for leavening. The
dough rises when yeasts give off CO2.
b. Yeasts ferment the carbohydrates of fruit to produce ethyl alcohol in wine, and
ferment grains to produce ethyl alcohol in beer.
c. The acetic acid bacteria, Acetobacter aceti, spoil wine, and convert the alcohol to
acetic acide to produce vinegar.
2. Bacterial Fermentation
a. Lactic acid bacteria cause milk to sour and produce yogurt, sour cream, and
cheese
b. Brine cucumber pickles, sauerkraut, and kimchi are pickled vegetables produced
by acid producing fermenting bacteria.
3. Soy Sauce Production
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a. Yeast and fermenting bacteria are added to soy beans and wheat to produce
soy sauce.
8.4 Inside the Mitochondria
1. The next reactions of cellular respiration involve the preparatory reaction, the
citric acid cycle, and the electron transport chain.
2. These reactions occur in the mitochondria.
3. A mitochondrion has a double membrane with an intermembrane space (between
the outer and inner membrane).
4. Cristae are the inner folds of membrane that jut into the matrix, the innermost
compartment of a mitochondrion that is filled with a gel-like fluid.
5. The prep reaction and citric acid cycle enzymes are in the matrix; the electron
transport chain is in the cristae.
6. Most of the ATP produced in cellular respiration is produced in the
mitochondria; therefore, mitochondria are often called the “powerhouses” of
the cell.
A. Preparatory Reaction
1. The preparatory reaction connects glycolysis to the citric acid cycle.
2. In this reaction, pyruvate is converted to a two-carbon acetyl group, and is
attached to coenzyme A, resulting in the compound acetyl-CoA.
3. This redox reaction removes electrons from pyruvate by a dehydrogenase
enzyme, using NAD+ as a coenzyme.
4. This reaction occurs twice for each glucose molecule.
5. CoA carries the acetyl group to the citric acid cycle.
6. The two NADH carry to electrons to the electron transport chain.
7. The CO2 diffuses out of animal cells into blood, transported to lungs, and
exhaled.
B. Citric Acid Cycle
1. The citric acid cycle occurs in the matrix of mitochondria.
2. The cycle is sometimes called the Krebs cycle, named for Sir Hans Krebs, who
described the fundamentals of the reactions in the 1930s.
3. The cycle begins by the addition of a two-carbon acetyl group to a four-carbon
molecule, forming a six-carbon citrate (citric acid) molecule.
4. In the subsequent reactions, at three different times two electrons and one
hydrogen ion are accepted by NAD+, forming NADH.
5. At one time, two electrons and one hydrogen ion are accepted by FAD, forming
FADH2.
6. NADH and FADH2 carry these electrons to the electron transport chain.
7. Some energy is released and is used to synthesize ATP by substrate-level
phosphorylation.
8. One high-energy metabolite accepts a phosphate group and transfers it to
convert ADP to ATP.
9. The citric acid cycle turns twice for each original glucose molecule.
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10. The products of the citric acid cycle (per glucose molecule) are 4 CO2, 2 ATP, 6
NADH and 2 FADH2.
11. Production of CO2
a. The six carbon atoms in the glucose molecule have now become the carbon
atoms of six CO2 molecules, two from the prep reaction and four from the
citric acid cycle.
C. The Electron Transport Chain
1. The electron transport chain is located in the cristae of mitochondria and
consists of carriers that pass electrons successively from one to another.
2. NADH and FADH2 carry the electrons to the electron transport system.
3. Members of the Chain
a. NADH gives up its electrons and becomes NAD+; the next carrier then gains
electrons and is thereby reduced.
b. At each sequential redox reaction, energy is released to form ATP
molecules.
c. Some of the protein carriers are cytochrome molecules, complex carbon
rings with a heme (iron) group in the center.
4. Cycling of Carriers
a. By the time electrons are received by O2, three ATP have been made.
b. When FADH2 delivers electrons to the electron transport system, two ATP
are formed by the time the electrons are received by O2.
c. Oxygen serves as the terminal electron acceptor and combines with
hydrogen ions to form water.
5. The Cristae of a Mitochondrion and Chemiosmosis
a. The electron transport chain consists of three protein complexes and two
protein mobile carriers that transport electrons.
b. The three protein complexes include NADH-Q reductase complex, the
cytochrome reductase complex, and the cytochrome oxidase complex; the
two protein mobile carriers are coenzyme Q and cytochrome c.
c. Energy released from the flow of electrons down the electron transport
chain is used to pump H+ ions, which are carried by NADH and FADH2, into
intermembrane space.
d. Accumulation of H+ ions in this intermembrane space creates a strong
electrochemical gradient.
e. ATP synthase complexes are channel proteins that serve as enzymes for
ATP synthesis.
f. As H+ ions flow from high to low concentration, ATP synthase synthesizes
ATP by the reaction: ADP + P = ATP.
g. Chemiosmosis is the term used for ATP production tied to an
electrochemical (H+) gradient across a membrane.
h. Once formed, ATP molecules diffuse out of the mitochondrial matrix
through channel proteins.
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i.
ATP is the energy currency for all living things; all organisms must
continuously produce high levels of ATP to survive.
D. Energy Yield From Glucose Metabolism
1. Substrate-Level Phosphorylation
a. Per glucose molecule, there is a net gain of two ATP from glycolysis in
cytoplasm.
b. The citric acid cycle in the matrix of the mitochondria produces two ATP
per glucose.
c. Thus, a total of four ATP are formed by substrate-level phosphorylation
outside of the electron transport chain.
2. ETC and Chemiosmosis
a. Most of the ATP is produced by the electron transport chain and
chemiosmosis.
b. Per glucose, ten NADH and two FADH2 molecules provide electrons and H+
ions to the electron transport chain.
c. For each NADH formed within the mitochondrion, three ATP are produced.
d. For each FADH2 formed by the citric acid cycle, two ATP are produced.
e. For each NADH formed outside mitochondria by glycolysis, two ATP are
produced as electrons are shuttled across the mitochondrial membrane by an
organic molecule and delivered to FAD.
3. Efficiency of Cellular Respiration
a. The energy difference between total reactants (glucose and O2) and
products (CO2 and H2O) is 686 kcal.
b. An ATP phosphate bond has an energy of 7.3 kcal; 36 to 38 ATP are
produced during glucose breakdown for a total of at least 263 kcal.
c. This efficiency is 263/686, or 39% of the available energy in glucose is
transferred to ATP; the rest of the energy is lost as heat.
8.5 Metabolic Pool
1. In a metabolic pool, substrates serve as entry points for degeneration or
synthesis of larger molecules.
2. Degradative reactions (catabolism) break down molecules; they tend to be
exergonic.
3. Synthetic reactions (anabolism) build molecules; they tend to be endergonic.
A. Catabolism
1. Just as glucose is broken down in cellular respiration, other molecules in the cell
undergo catabolism.
2. Fat breaks down into glycerol and three fatty acids.
a. Glycerol is converted to G3P, a metabolite in glycolysis.
b. An 18-carbon fatty acid is converted to nine acetyl-CoA molecules that
enter the citric acid cycle.
c. Respiration of fat products can produce 108 kcal in ATP molecules; fats are
an efficient form of stored energy.
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3. Amino acids break down into carbon chains and amino groups.
a. Hydrolysis of proteins results in amino acids.
b. R-group size determines whether carbon chain is oxidized in glycolysis or the
citric acid cycle.
c. A carbon skeleton is produced in the liver by removal of the amino group, by
the process of deamination.
d. The amino group becomes ammonia (NH3), which enters the urea cycle and
ultimately becomes part of excreted urea.
e. The size of the Regroup determines the number of carbons left after
deamination.
B. Anabolism
1. ATP produced during catabolism drives anabolism.
2. Substrates making up pathways can be used as starting materials for synthetic
reactions.
3. The molecules used for biosynthesis constitute the cell’s metabolic pool.
4. Carbohydrates can result in fat synthesis: G3P converts to glycerol, acetyl
groups join to form fatty acids.
5. Some metabolites can be converted to amino acids by transamination, the
transfer of an amino acid group to an organic acid.
6. Plants synthesize all the amino acids they need; animals lack some enzymes
needed to make some amino acids.
7. Humans synthesize 11 of 20 amino acids; the remaining 9 essential amino acids
must be provided by the diet.
C. The Energy Organelles Revisited
1. Chloroplasts and mitochondria may be related based on their likeness, yet they
carry out opposite processes.
a. The inner membrane of the chloroplasts forms the thylakoids of the grana.
The inner membrane of the mitochondrion forms the convoluted cristae.
b. In chloroplasts the electrons passed down the ETC have been energized by
the sun. In mitochondria the electrons passed down the ETC have been
removed from glucose products.
c. In chloroplasts the stroma contains the enzymes of the Calvin cycle. In the
mitochondria the matrix contains the enzymes of the citric acid cycle.
2. Flow Of Energy
a. Energy flows through organisms. For example, the sun is the energy source
for producing carbohydrates in chloroplasts. In the mitochondria, the
carbohydrate energy is converted into ATP molecules during cellular
respiration.
b. Chemicals cycle throughout cells. Mitochondria use carbohydrates and
oxygen produced in chloroplasts, and chloroplasts use carbon dioxide and
water produced in the mitochondria.
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Chapter 8: Cellular Respiration
Cellular Respiration
1. Write the overall reaction for glucose breakdown and show that it is a redox
reaction.
2. Discuss the role of oxidation-reduction enzymes.
3. State the four phases of cellular respiration and tell where each occurs in
the cell.
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Outside the Mitochondria: Glycolysis
4. Contrast the energy-investment step of glycolysis with the energyharvesting steps.
5. Summarize glycolysis by stating the inputs and outputs of the pathway.
Fermentation
6. Explain the benefits and drawbacks of fermentation.
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Inside the Mitochondria
7. Show that glucose products are broken down completely during the
preparatory reaction and the citric acid cycle.
8. Give the net gain of substrate level ATP synthesis and NADH as a result of
these pathways.
9. Describe how the cristae are organized to produce ATP.
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Metabolic Pool
10. Show how catabolism of protein and fat utilizes the same pathways as
glucose breakdown.
11. Compare and contrast the organization and structure of mitochondria and
chloroplasts and how they permit a flow of energy through living things.
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CELLULAR RESPIRATION
STEP 1: GLYCOLYSIS
GLUCOSE
CCCCCC
2 ATP add 2 phosphates to the
glucose, energizing it.
Glucose diffuses
into the cytoplasm
from the blood
stream.
2 ATP
2 ADP
P–CCCCCC-p
(G1phosphate)
P–CCC
Glucose1,6 phosphate splits,
creating 2PGAL molecules.
CCC–P
(G3phosphate)
P
P
An additional phosphate is added
to each PGAL.
Each 3C
molecule now has
a 2nd phosphate
added.
The NADH
& H+ are
energy
carriers
P–CCC-P
P – C C C -P
High Energy electrons & hydrogen
atoms removed from 3C are used
to create NADH.
1 NAD +
1 NADH +
H+
2 ADP
High energy phosphates removed
from 3C are used to make ATP.
2ATP
ATP
1 NAD +
1 NADH + H+
2 ADP
2ATP
Glycolysis
makes
4ATP(2ATP)
net)
CCC
CCC
These 2 Pyruvates finishes
glycolysis!
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SUMMARY
Preamble
ATP is used for:
1. Chemical work
Such as during digestive processes.
2. Transport work
Such as transporting substances across membranes.
3. Mechanical work
Such as beating of cilia and muscle contraction.
Cellular respiration produces ATP. It is an exergonic reaction. Delta G = -2870 KJ/mol or 686 kcal/mol.
Cells recycle the ATP they use for work. Cellular respiration transfers energy stored in food
to molecules of ATP.
ATP is a nucleotide with high energy phosphate bonds that the cells hydrolyze for energy to
drive other endergonic reactions.
The terminal phosphate group from ATP contains all the energy. This phosphate is
transferred to other compounds in a process called phosphorylation.
The molecule acquiring the phosphate is said to be phosphorylated.
Redox reactions.
Oxidation-reduction reactions= chemical reactions which involve a partial or complete
transfer of electrons from one reactant to another. This is called Redox Rx for short.
Oxidation= partial or complete loss of electrons.
Reduction= partial or complete gain of electrons.
This reaction requires both a donor and an acceptor. So when one reactant is oxidized the
other is reduced.
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• Examples of redox reactions
becomes oxidized
(loses electron)
Na
+
Na+
Cl
+
Cl–
becomes reduced
(gains electron)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Oxidation
CO2 + 2H2O
CH4 + 2O2
Reduction
Oxidation
Xe + Y
X
Reduction
Where:
X = substance being oxidized
It is a reducing agent. Since it reduces y.
Y = substance being reduced.
It is an oxidizing agent. Since it oxidizes x
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+ Ye
Cellular respiration as a Redox process.
Apply this rule to cellular respiration.
Oxidation
C6H12O6 + 602
6CO2+ 6H2O+Energy
Reduction
THE PROCESS OF CELLULAR RESPIRATION- HARVESTING CELLULAR ENERGY
• Energy
– Flows into an ecosystem as sunlight and
leaves as heat
Light energy
ECOSYSTEM
Photosynthesis
in chloroplasts
Organic
CO2 + H2O
+ O2
Cellular
molecules
respiration
in mitochondria
ATP
powers most cellular work
Figure 9.2
Heat
energy
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
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• An overview of cellular respiration
Electrons carried
via NADH and
FADH2
Electrons
carried
via NADH
Cytosol
Mitochondrion
ATP
Figure 9.6
Oxidative
phosphorylation:
electron
transport and
chemiosmosis
Citric
acid
cycle
Glycolsis
Pyruvate
Glucose
Substrate-level
phosphorylation
ATP
ATP
Substrate-level
phosphorylation
Oxidative
phosphorylation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
C6H12O6
+ 6O2
Organic compound + oxygen
(food)
6CO2 +
6H2O + ATP
Carbon dioxide + water + energy
Cellular respiration actually involves THREE steps:
1. Glycolysis…….1b…..Connecting Step
2. Krebs Cycle
3. Electron Transport Chain/Oxidative Phosphorylation
1. Glycolysis;
This is a catabolic pathway
Involves 10 steps. Each step requires specific enzymes.
The first five steps is called the energy-investment phase (because ATP is used) while the last
five steps are called the energy- yielding phase.
Oxygen is not used. It is therefore considered to be anaerobic
One molecule of glucose produces 2-3C molecules called pyruvate.
Needs an investment of 2 ATP to start reaction.
A total of 4 ATP molecules are produced.
A net of 2 ATP molecules are produced.
2 NADH and 2 H ions are also produced.
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This takes place in the cytosol of the cytoplasm, of ALL cell types(prokaryotes and
Eukaryoyes)
Glucose (6C)
NADH
ATP
3C
3C
Pyruvic Acid
1b. Connecting Step:
2 pyruvates enter the mitochondrial matrix.
The 2 pyruvates are converted to 2 acetyl coenzyme A.
No ATP is produced.
2 CO2 are produced.
2 NADH are produced and 2 H+ are produced.
Oxygen is not used. It is therefore considered to be anaerobic
This takes place in the mitochondrial matrix.
2. Krebs Cycle; also known as the Citric Acid Cycle or the Tricarboxylic Acid Cycle.
This is a catabolic pathway
Completes glucose oxidation by breaking down pyruvate derivative (acetyl CoA) into carbon
dioxide.
For every glucose molecule, Krebs cycle goes around twice. -One for each acetyl Co A.
Each step requires a specific enzyme.
Oxygen is not used.
2 ATPs- 1(one) from each cycle is produced.
4 CO2-2 from each cycle are produced.
8 NADH-4 from each cycle are produced.
2 FADH2-1 from each cycle are produced.
This takes place in the cytoplasm (Prokaryotes)
But takes place in the mitochondrial matrix (Eukaryotes).
3. Electron Transport Chain and Oxidative Phosphorylation/Chemiosmosis Synthesis
The ETC is made of electron carrier molecules embedded in the inner mitochondrial membrane.
Except for ubiquinone (Q) most of the carrier molecules are proteins and are tightly bound to
prosthetic groups (nonprotein cofactors).
The inner mitochondrial membrane has a chain of electron transport proteins.
NADH and FADH2 are coenzymes that have high energy electrons.
These high energy electrons are passed through the chain of electron transport proteins.
Finally they will be passed on to oxygen. This is called the final electron acceptor. Oxygen will
then combine with 2 hydrogen ions to form water.
The energy released during this transfer of electrons through the electron transport chain is used
to pump H+ from the mitochondrial matrix into the inner membrane space. Now there is H+
gradient established between the intermembrane space (greater) and the matrix (lesser)
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H+ therefore will move from intermembrane space back to the matrix through a special protein
complex located in the inner mitochondrial membrane called an ATP synthase.
This flow of H+ will activate this enzyme and subsequently produce 6H2O and 34 ATPs.
Produces 90% of the ATP.
This is the chemiosmotic synthesis of ATP.
This step uses oxygen.
Most of the ATP of the body is produced here.
Takes place in the cell membrane (Prokaryotes)
Takes place in the inner mitochondrial membrane (Eukaryotes).
Oxidative phosphorylation
This is ATP production that is coupled to the exergonic transfer of electrons from food to
oxygen.
A small amount of ATP is produced directly by the reactions of glycolysis and Krebs cycle.
This mechanism of producing ATP is called substrate level phosphorylation.
Substrate level phosphorylation
This is ATP production by direct enzymatic transfer of phosphate from an intermediate
substrate in catabolism to ADP.
Glycolysis
Is a catabolic reaction during which 6-C Glucose is split into 2-3Csugars called pyruvates?
This reaction may take place in the presence or absence of oxygen.But generally considered
to be anaerobic.
Energy investment phase
The cell uses ATP to phosphorylate the intermediates of glycolysis.
2 ATP molecules are invested
Energy yielding phase
2- 3C intermediates are oxidized. For each glucose molecule entering glycolysis a net gain
of ATP is produced.
2 ATP molecules are therefore yielded.
Heme group
Prosthetic group composed of four organic rings surrounding a single iron atom.
Cytochrome
Type of protein molecule that contains a Heme prosthetic group and functions as an
electron carrier in the electron transport chain of mitochondria and chloroplast.
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FMN= Flavin mononucleotide
NAD= Nicotinamide Adenine Dinucleotide
BREAKDOWN OF ATP PRODUCED:
Glycolysis:
4 ATP are produced/2 net ATP
None from connecting step
Krebs cycle:
2 ATP are produced
Electron Transport Chain (chemiosmosis):
34 ATP are produced
TOTAL----38 (40) ATPs are produced.…………………………………
INTERFERENCE:
A number of poisons may interfere with the chemiosmosis mechanism.
There are 3 types (classes) of respiratory poisons:
a. cyanide
These types of poisons will block the passage of electrons from cytochrome a3 to oxygen. As
a result no protons are pumped and no ATP is made
b. Inhibitors
These types of poisons inhibit ATP synthesis. An example are antibiotics e.g. oligomycin
c. uncouplers
These types of poisons short circuit the proton current by making the lipid bilayer leaky to H
ions. This will in turn make it impossible to establish a proton gradient and so no ATP will
be made.
Metabolic Processes
Food can be oxidized under different conditions.
1. Aerobic
The breakdown of food in the presence of oxygen.
2. Anaerobic
The breakdown of food in the absence of oxygen.
3. Fermentation
Anaerobic breakdown of food.
4. Facaultative
Breakdown of food in the presence or absence of oxygen.
Organisms may be classified based on the effect oxygen has on their growth and metabolism.
1. STRICT OBLIGATE AEROBES
Organisms that require oxygen for growth development.
example, humans and Bacillus bacteria
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2. STRICT OBLIGATE ANAEROBES
Organisms that only grow in the absence of oxygen and are in fact poisoned by it.
Example, Bacteroides fragilis and Clostridium botulinum
3. FACULTATIVE ANAEROBES
Organisms that may grow in the presence or absence of oxygen. Examples include yeast,
muscle cells and bacteria.
Many yeasts, enteric bacteria, such as the Gram-negative Escherichia coli, and skin-dwelling
Gram-positive halophiles, like Staphylococcus, are facultative anaerobes
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