Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
FIRST SEMESTER – APRIL 2010
BT 1822 - IMMUNOLOGY & IMMUNOTECHNOLOGY
Date & Time: 29/04/2010 / 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART – A (20 MARKS)
Answer ALL the questions
I. Choose the Correct answer.
(5x1=5)
1. Which of the following is the most striking of all test-tube reactions?
a) Precipitation b) Agglutination
c) Complement Fixation
d) Blotting
2. Out of the following, which is not a part of myeloid lineage of cells?
a) monocytes
b) megakaryocyte
c) NK cells d) Langerhan cells
3. The type of rejection occurring within hours of transplantation is called as
a) Hyperacute b) acute
c) chronic
d) lethal
4. Which of the following molecule is involved in exogenous mode of antigen processing?
a) Tapasin
b) Calreticulin
c) Calnexin
d) CLIP
5. ISCOM (Immune Stimulating Complexes ) are antigens in ______ cover.
a) carbohydrate b) lipid
c) protein
d) nucleic acid
II. State whether the following are True or False; state reason
6. CD 45 is a signal transduction molecule found on B lymphocytes.
7. Dendritic cell is not an example of professional antigen presenting cell.
8. Apoptosis is a physiological phenomena rather than a pathological one.
9. CRP is an acute phase protein that does not mediate inflammatory response.
10. Phage Display Libraries use the Gene III-surface expression protein of M13.
(5x1=5)
III. Complete the following:
(5x1=5)
11. Haptens possess the property of _______ but not ________.
12. ADEPT stands for _________________________________.
13. The macrophages present in liver and _____are called as ______ and osteoclasts respectively.
14. p53 is an example of _________ gene.
15. _________ is the widely used fusgen in hybridoma experiments.
IV. Answer the following in one or two sentences, each in about 50 words
16. Give the technical definition of an antigen.
17. What is Titre value?
18. Define the term: Affinity Maturation
19. Differentiate between diagnostic sensitivity and specificity.
20. What are Plantibodies?
(5x1=5)
PART – B
V. Answer any FIVE questions, each in about 350 words only
(5 x 8 = 40 marks)
21. Distinguish between the epitopes recognized by B and T cells. Add a note on epitope mapping.
22. What are Cytokines? Enlist their general properties and write about the groups of cytokines. Add a
note on cytokine therapy.
23. How does gene rearrangement prove helpful in generating a diverse repertoire of antibody in our
system?
24. (i) Give a schematic description of Cell-mediated immunity.
(ii) Give a diagrammatic description of both positive and negative Complement Fixation Tests.
25. Write about competitive and sandwich types of ELISA.
26. Give an account on the clinical applications of monoclonal antibodies with suitable examples.
27. What are the various forces operative during the antigen-antibody interactions?
28. Explain the construction and applications of Flow Cytometry.
PART – C
IV. Answer the following, each in about 1500 words.
(2 x 20 = 40 marks)
29. a) Explain in detail about the general structure of an immunoglobulin molecule, with diagram.
Tabulate the differences between the five different types of antibodies.
(or)
b) Write an essay on AIDS, focusing upon the structure of the virion, pathogenesis, clinical
manifestations, immunological abnormalities, mode of transmission, opportunistic infections,
laboratory diagnosis and prophylaxis, if any.
30. a) Give a detailed account on the developments in the field of vaccinology, starting from Jenner’s
period till date, mentioning the pros and cons at each stage of the advancements.
(or)
b) Bring out the recent trends related to antibody engineering. How do you validate an immunoassay
before clinical trials?
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