Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
THIRD SEMESTER – NOVEMBER 2014
BT 3825 - BIOPROCESS & PHARMACEUTICAL TECHNOLOGY
Date : 07/11/2014
Time : 09:00-12:00
Dept. No.
Max. : 100 Marks
PART – A
Answer ALL the Questions:
I Choose the correct answer
(20 Marks)
(5x1 = 5 Marks)
1. ____________considered fermentation as a chemical process.
a) Pasteur
b) Liebig
c) Jenner
2. ___________ is the cheapest source of carbohydrates.
a) molasses
b)malt extract c) sugar beet
d) Fleming
d) corn steep liquour
3. A reaction in which one of the products of the reaction increases the overall rate of the reaction
______________.
a) photocatalytic
b) monocatalytic
c) autocatalytic
d) pseudocatalytic
4. Rennet causes the milk to
a) condense
b) flocculate
c) coagulate
d) stabilise
5. Rituximab is used in the treatment of
a) Arthritis
b) Cancer
c) Haemophilia
d) Jaundice
II State whether the following statements are True or False, if false give reasons:
(5x1 = 5 Marks)
6. Cell separation is not done in downstream processing.
7. Yeast is used in bread and alcohol production.
8. Biotin is a cofactor used by enzymes which carboxylate substrates.
9. Dendrimers are polymer based drug delivery vehicles.
10. The limiting factor for the growth of algae for SCP production on a large scale is illumination.
III Complete the following:
(5x1= 5 Marks)
11. _______________is used to introduce small bubbles into the fermenter.
12. Hydrocarbon of interest in bacterial SCP production is _________.
13. _________________is used in the industrial production of lactic acid .
14. ________________are starch rich materials added during beer preparation.
15. ICMR stands for _________________ .
IV Answer the following,each within 50 words:
(5x1 = 5 Marks)
16. What is headspace and working volume in a bioreactor?
17. Differentiateprobiotics from prebiotics.
18. What is Kefir?
19. Mention the use of antifoam agents.
20. When is scaling down done?
PART B
Answer the following each within 500 words. Draw diagrams wherever necessary:
(5x8 = 40 marks)
21. (a)Discuss briefly the considerations made when formulating media for fermentation.
OR
b) What is sterilization? Discuss the different sterilization methods in fermentation
technology.
22. (a) Discuss briefly primary screening of industrially important microbes with examples.
OR
(b) Write a short note on the different methods of continuous culture.
23. (a) Explain the principle behind liquid-solid and liquid – liquid extraction
OR
(b)Discuss briefly the variations in the production of cheese.
24. (a) Discuss briefly the different recombinant enzymesand hormones in treating diseases.
OR
(b) Write a short note on production of spirulina.
25. (a) What is a clinical trial? Discuss the different phases of clinical trial.
OR
(b) Enumerate the pharmaceutical applications of steroids and prostaglandins.
PART – C
Answer any two of the following, each within 1500 words. Draw diagrams wherever necessary.
(2 x20 = 40 Marks)
26. Give a detailed account of the different types of Bioreactors.
27. Explain the natural gene transfer methods for strain improvement.
28. Write in detail the need and types of bioanalytical method validation.
29. Explain indetail on targeted drug delivery systems.
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