Download Pfizer Technologies and Resources Accessible to Investigators and Projects

Pfizer Technologies and Resources Accessible to Investigators and Projects
Technologies at CTI-SF Site
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Monoclonal antibody therapeutics
 Human monoclonal antibody via phage display technology
– Large 1010 libraries
– Both naive and semi-synthetic libraries
 Human monoclonal antibody from human IgG transgenic mice
 Humanized rodent monoclonal antibody via hybridoma technology
– Robust panel (10-20) of mAbs for each target
– Exhaustive and innovative immunization approaches to ensure success
Non-antibody biotherapeutics
 Fc fusion protein
 Secreted protein
Peptide therapeutics
Novel biotherapeutic modalities
 Bispecific antibodies
 Antibody drug conjugates (ADCs)
 CovX bodies
Humanization of rodent mAbs
 Reduce rodent sequence to <5%
Affinity maturation
 Increase antigen binding affinity by 10-100 fold
Half-life extension
 Modulation of IgG binding to FcRn
– Increase t1/2 by 3 fold
 PEGylation
Fc engineering
 Decrease ADCC and CDC
 Increase ADCC
Knowledge-based protein engineering
 Structural analysis
 Molecular modeling
Small-medium protein expression and characterization of proteins
 Proprietary expression vector systems and efficient purification process
 Biochemical and biophysical analysis
Technologies at Other Pfizer Sites Accessible to CTI-SF Collaborations
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Systems biology
 Transcriptional profiling
 Proteomic studies
 Computational analysis of biological networks and pathways
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Genetic and genomic analysis
 Deep sequencing
Biological models
 Genetically modified animals
 Stem cell models
 Human clinical samples
State-of-the-art imaging technologies
Protein structural studies and molecular modeling
Large-scale expression and purification of biotherapeutic candidates
 Proprietary expression vector system
 Mammalian expression system for the generation of hundreds of milligrams
to grams of proteins
 Sophisticated purification methods to obtain high-quality purified proteins
Robust analytic methods
 Biochemical analysis
 Biophysical analysis
PK/PD
 Rodents and non-human primates
 PK/PD modeling
 Immunogenicity prediction
Toxicology
 Rodents and non-human primates
 Tissue bank analysis of target expression
 IND-enabling toxicology studies
GMP clinical grade manufacturing
 Proprietary technologies for efficient process
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