Download 22/18 INVESTIGATOR Name Jeremy P. Brockes Address Ludwig

22/18
INVESTIGATOR
Name
Jeremy P. Brockes
Address Ludwig Institute for Cancer Research, Middlesex Hospital, University College Branch, Courtauld Building, 91 Ridding
House Street, London W1P 8BT, United Kingdom
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
early to mid-bud stage blastemas
Notophthalmus viridescens
adult newt after amputation through upper forelimb
mouse
BALB/cJ
female
spleen
50-100 g of protein for priming and for several boosts before sacrificing
i.p.
none
December, 1982
mouse
NS1/SP2
IgM
see papers for details of developing and regenerating limbs and normal embryos
Notophthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher
vertebrates.
blastemal cells
ANTIGEN
Chemical properties
Molecular weight
Characterization
Immunoprecipitation
Immunoblotting
unsuccessful
Purification
Amino acid sequence analysis
Functional effects
Immunohistochemistry
The antibody recognizes a cytoplasmic component of a population of blastemal cells that is lost
when the cells differentiate into mature tissues. The growth of this population of cells is nerve dependent. (Intermediate
filament protein or intermediate filament associated protein when staining fixed cultured blastemal cells.)
PUBLICATIONS :
Kintner, C.R., and Brockes, J.P. (1984). Monoclonal antibodies identify blastemal cells derived from dedifferentiating muscle in
newt limb regeneration. Nature 308,
67-69.
Kinter, C.R., and Brockes, J.P. (1985). Monoclonal antibodies to the cells of a regenerating limb. J. Embryol. exp. Morph. 89, 3755.
(Continued)
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Brockes, J.P., and Kintner, C.R. (1986). Glial growth factor and nerve-dependent proliferation in the regeneration blastema of
urodele amphibians. Cell 45, 301-306.
Fekete, D.M., and Brockes, J.P. (1987). A monoclonal antibody detects a difference in the cellular composition of developing and
regenerating limbs of newts.
Development 99, 589-602.
Griffin, K.J.P., Fekete, D.M., and Carlson, B.M. (1987). A monoclonal antibody stains myogenic cells in regenerating newt muscle.
Development 101, 267-277.
Young, H.E., Sippel, J., Putnam, L.S., Lucas, P.A., and Morrison, D.C. (1992). Enzyme-linked immuno-culture assay. J. Tiss. Cult.
Meth. 14, 31-36.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242