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zn-1 (Only cell products will be distributed.) INVESTIGATOR Name Bill Trevarrow Address Institute of Neuroscience, University of Oregon 1254; Eugene, OR 97403-1254 E-Mail [email protected] IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen (CC) Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity 1-6: 2 day old ZF embryo membranes Zebrafish (ZF) mouse Balb/c female spleen 1 13vi84 0.5 IP Freund’s 2 16vii84 0.5 IP Freund’s 3 12viii84 0.5 IP Freund’s 4 18xii84 0.4 IP Freund’s 5 1vii85 0.25/IP 0.05/IV none 6 3vii85 0.05 IV none 4vii85 mouse SP 2/0 IgG1, mouse cytoplasmic good, all neurons, hair cells not tried Pos.: zebrafish embryos/larva (2d-4d) Neg.: Xenopus, betta, trout, medaka, shark, chick, Haplochromis burtoni ANTIGEN Chemical properties ? Molecular weight ~43kDa Characterization Immunoprecipitation not done Immunoblotting yes Purification not done Amino acid sequence analysis Functional effects not tried Immunohistochemistry 4% paraformaldehyde, 4C, ON, cryostat, or wholemount PUBLICATIONS : Hanneman, E., Trevarrow, B., Metcalfe, W. K., Kimmel, C. B., and Westerfield, M. (1988). Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo. Development 103, 49-58. Trevarrow, B., Marks, D.L., and Kimmel, C.B. (1990). Organization of hindbrain segments in the zebrafish embryo. Neuron 4, 669679. (Continued) zn-1 (Continued) Kornblum, H.I., Corwin, J.T., and Trevarrow, B. (1990). Selective labeling of sensory hair-cells and neurons in auditory, vestibular, and lateral line systems by a monoclonal antibody. J. Comp. Neur. 301(2), 162-170. Larison, K. and Trevarrow, B. (sources)- (1994). Zebrafish monoclonal antibodies. in: The Zebrafish Book. Monte Westerfield University of Oregon Press; edition 2.1. Hutchinson, S.A., and Eisen, J.S. (2006). Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development 133, 2137-2147. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242