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Download 62.1E6 INVESTIGATOR Name Thomas M. Jessell and
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62.1E6 INVESTIGATOR Name Thomas M. Jessell and Susan Brenner-Morton Address Howard Hughes Medical Institute, Columbia University, 701 W. 168th St., New York, NY 10032 IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity Slug (chick) bacterial expression 6-his full length protein mouse Balb/c female spleen 100 µg IP CFA + IFA Balb/c NS-1 IgG1, kappa light chain nuclear neural crest chick, Xenopus ANTIGEN Chemical properties protein/transcription factor Molecular weight ~30 kDa Characterization Immunoprecipitation Immunoblotting Purification Amino acid sequence analysis Functional effects Immunohistochemistry PUBLICATIONS : Liem, Jr., K.F., Tremml, G., Roelink, H., and Jessell, T.M. (1995). Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm. Cell 82, 969-979. Liem, Jr., K.F., Tremml, G., and Jessell, T.M. (1997). A role for the roof plate and its resident TGFß-related proteins in neuronal patterning in the dorsal spinal cord. Cell 91, 127-138. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242