Download LA4 INVESTIGATOR Name Thomas M. Jessell and Jane Dodd

LA4
INVESTIGATOR
Name
Thomas M. Jessell and Jane Dodd
Address Center for Neurobiology & Behavior, Columbia University, 722 W. 168th St., New York, NY 10032
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
AR42J cells
mouse
BALB/c
female
spleen
10/5/83, 10/19/83, 11/11/83
4.5 x 107 whole cells
i.p.
complete Freund’s adjuvant followed by incomplete Freund’s adjuvant
11/14/83
mouse
NS1
MONOCLONAL ANTIBODY
Isotype
IgM
Specificitycell binding: AR42J cells, human RBC/B group antigen
Cell binding
Immunohistology
Antibody competition
Species Specificity
rodent
ANTIGEN
Chemical properties
glycolipid; the epitope is an alpha-galactose extended lactoseries carbohydrate that may also
Molecular weight
be present on proteins
Characterization
Immunoprecipitation
Immunoblotting
several bands on TLC
Purification
Amino acid sequence analysis
Functional effects
Immunohistochemistry
subsets of peripheral sensory structures
PUBLICATIONS :
Dodd, J., and Jessell, T.M. (1985). Lactoseries carbohydrates specify subsets of dorsal root ganglion neurons projecting to the
superficial dorsal horn of rat spinal cord. J. Neurosci. 5, 3278-3294.
Chou, D.K.H., Dodd, J., Jessell, T.M., Costello, C.E., and Jungalwala, F.B. (1989). Identification of alpha-galactose
(alpha-fucose)-asialo-GM1 glycolipid expressed by subsets of rat dorsal ganglion neurons. J. Biol. Chem. 264, 3409-3415.
Lee, C.J., Kong, H., Manzini, M.C., Albuquerque, C., Chao, M.V., and MacDermott, A.B. (2001). Kainate receptors expressed by a
subpopulation of developing nociceptors rapidly switch from high to low Ca2+ permeability. J. Neurosci. 21(13), 4572-4581.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242