Download AG1 INVESTIGATOR Name Miyuki Yamamoto Address

AG1
INVESTIGATOR
Name
Miyuki Yamamoto
Address Inst. Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 JAPAN
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
embryonic brain
Sprague-Dawley rat
E15-17
mouse
balb/c
female
spleen
unknown
several embryonic brains per injection
intraperitoneal
poly I poly C
unknown
mouse
NS-1
IgG2b
unknown
+
unknown
mouse and rat, other species have not been tested
ANTIGEN
Chemical properties
NCAM
Molecular weight
140 kDa and 180 kDa
Characterization
Immunoprecipitation
Immunoblotting
blots well
Purification
Amino acid sequence analysis
Functional effects
not tested
Immunohistochemistry
studied
PUBLICATIONS :
DiFiglia, M., Marshall, P., Covault, J., and Yamamoto, M. (1989). Ultrastructural localization of molecular subtypes of
immunoreactive neural cell adhesion molecule (NCAM) in the adult rodent striatum. J. Neurosci. 9, 4158-4168.
Dedkov, E.I., Borisov, A.B., Wernig, A., and Carlson, B.M. (2003). Aging of skeletal muscle does not affect the response of satellite
cells to denervation. J. Histochem. Cytochem. 51(7), 853-863.
Keller, C., Hansen, M.S., Coffin, C.M., and Capecchi, M.R. (2004). Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function:
implications for alveolar rhabdomyosarcoma cell of origin. Genes Dev. 18, 2608-2613.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242