Download CaF2-5D2 INVESTIGATOR Name Douglas M

CaF2-5D2
INVESTIGATOR
Name
Douglas M. Fambrough
Address Department of Biology, The Johns Hopkins University, 34th & Charles Streets, Baltimore, MD 21218
E-Mail [email protected]
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
chicken pectoralis muscle SR vesicle prep
chicken
SR membranes
adult
mouse
BALB/cJ
spleen
200 g SR prep
intraperitoneal; tail vein boost 7, 14, & 21 days later
Freund's complete
mouse
SP2/0 Ag8
IgG1, kappa light chain
B tryptic fragment (45 kDa) of SERCA1 (fast twitch SR Ca+2-ATPase)
all vertebrates tested to date
ANTIGEN
Chemical properties
Ca+2 SR ATPase fast twitch muscle
Molecular weight
110 kDa
Characterization
Immunoprecipitation
Immunoblotting
0.5 g/ml very good
Purification
Amino acid sequence analysis
Functional effects
unknown
Immunohistochemistry
very good on cryosections and formaldehyde or methanol fixed tissues 5 g/ml
PUBLICATIONS :
Airey, J., Baring, M., Beck, C., Chelliah, Y., Deerinck, T., Ellisman, M., Houenou, L., McKemy, D., Sutko, J., and Talvenheimo, J.(1993). Failure to
make normal  ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken. Devel. Dyn. 197, 169-188.
Airey, J., Deerinck, T., Ellisman, M., Houenou, L., Ivaneko, A., Kenyon, J., McKemy, D., and Sutko, J. (1993). Crooked neck dwarf (cn) mutant
chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant
phenotype. Devel. Dyn. 197, 189-202.
(Continued)
CaF2-5D2 (Continued)
Murphy, R.M., Larkins, N.T., Mollica, J.P., Beard, N.A., and Lamb, G.D. (2009). Calsequestrin content and SERCA determine normal and maximal
Ca2+ storage levels in sarcoplasmic reticulum of fast- and slow-twitch fibres of rat. J. Physiol. 587.2, 443-460.
Murphy, R.M., and Lamb, G.D. (2009). Endogenous Calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is
not dependent on stretch. J. Biol. Chem. 284(12), 7811-7819.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained
from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa,
Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242