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F-11 INVESTIGATOR Name Henry F. Epstein Address Dept of Neuroscience and Cell Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0625 (This antibody was prepared in the laboratory of Kimon J. Angelides, formerly of the Baylor College of Medicine, Houston, TX.) IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species mouse Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity IgG1 C. elegans body-wall muscle cells and intestinal cells; embryos and starved free-living C. elegans ANTIGEN glyoxylate cycle protein (isocitrate lyase-malate synthase) Chemical properties see Liu et al, publications Molecular weight 106 kDa Characterization Immunoprecipitation Immunoblotting Purification Amino acid sequence analysis Functional effects Immunohistochemistry PUBLICATIONS : Liu, F., Thatcher, J.D., Barral, J.M. and Epstein, H.F. (1995). Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: A developmentally regulated protein of intestine and muscle. Dev. Biol. 169, 399-414. Liu, F., Thatcher, J.D., and Epstein, H.F. (1997). Induction of glyosylate cycle expression in Caenorhabditis elegans: A fasting response throughout larval development. Biochemistry 36, 255-260. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242