Download F-11 INVESTIGATOR Name Henry F. Epstein Address

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts

Biochemical cascade wikipedia , lookup

Biomolecular engineering wikipedia , lookup

Cell culture wikipedia , lookup

Expression vector wikipedia , lookup

Organ-on-a-chip wikipedia , lookup

Cell theory wikipedia , lookup

Protein purification wikipedia , lookup

Protein–protein interaction wikipedia , lookup

Life wikipedia , lookup

Protein adsorption wikipedia , lookup

DNA vaccination wikipedia , lookup

Biochemistry wikipedia , lookup

History of molecular biology wikipedia , lookup

Two-hybrid screening wikipedia , lookup

History of biology wikipedia , lookup

Chemical biology wikipedia , lookup

Polyclonal B cell response wikipedia , lookup

Western blot wikipedia , lookup

Developmental biology wikipedia , lookup

Biology wikipedia , lookup

Monoclonal antibody wikipedia , lookup

Transcript
F-11
INVESTIGATOR
Name
Henry F. Epstein
Address Dept of Neuroscience and Cell Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX
77555-0625
(This antibody was prepared in the laboratory of Kimon J. Angelides, formerly of the Baylor College of Medicine, Houston, TX.)
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
mouse
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
IgG1
C. elegans body-wall muscle cells and intestinal cells; embryos and starved free-living
C. elegans
ANTIGEN
glyoxylate cycle protein (isocitrate lyase-malate synthase)
Chemical properties
see Liu et al, publications
Molecular weight
106 kDa
Characterization
Immunoprecipitation
Immunoblotting
Purification
Amino acid sequence analysis
Functional effects
Immunohistochemistry
PUBLICATIONS :
Liu, F., Thatcher, J.D., Barral, J.M. and Epstein, H.F. (1995). Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: A
developmentally regulated protein of intestine and muscle. Dev. Biol. 169, 399-414.
Liu, F., Thatcher, J.D., and Epstein, H.F. (1997). Induction of glyosylate cycle expression in Caenorhabditis elegans: A fasting
response throughout larval development. Biochemistry 36, 255-260.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242