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Transcript
Bio260 – Spring 2014
In-class activity
Metabolism, Part II
HARVEST ENERGY, MAKE PRECURSORS: Now that the nutrients are inside the cell, the cell must harvest
energy and make precursor metabolites from those nutrients to be able to grow.
 Part A: Harvest energy - Draw a flow chart or diagram that shows how energy is harvested
inside each of the following different chemoorganoheterotrophic bacteria using the different
catabolic pathways in the table below.
o Obligate aerobe
o Facultative anaerobe
o Obligate anaerobe
 Table of catabolic processes
o The central metabolic pathways
 Glycolysis
 Pentose phosphate pathway
 Tricarboxylic acid cycle (TCA cycle) and transition step
o Aerobic respiration
o Anaerobic respiration
o Fermentation
 Part B: Now label on each diagram how the harvested energy is stored during each catabolic
process.
 ATP
• Substrate-level phosphorylation and/or
• Oxidative phosphorylation
 Proton motive force
• Electron transport chain
o Oxidation-reduction (redox) reactions
o Active transport
o Proton (H+) concentration gradient
 Reducing power
• Electron carriers
o NADH, FADH2, NADPH
 Part C: Make precursor metabolites and their subunits. Now for the last part of catabolism, we
need to understand that precursor metabolites are made by the central metabolic pathways and are
used to make subunits. Label on your diagram where you would expect precursor metabolites to be
made.