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Bio260 – Spring 2014 In-class activity Metabolism, Part II HARVEST ENERGY, MAKE PRECURSORS: Now that the nutrients are inside the cell, the cell must harvest energy and make precursor metabolites from those nutrients to be able to grow. Part A: Harvest energy - Draw a flow chart or diagram that shows how energy is harvested inside each of the following different chemoorganoheterotrophic bacteria using the different catabolic pathways in the table below. o Obligate aerobe o Facultative anaerobe o Obligate anaerobe Table of catabolic processes o The central metabolic pathways Glycolysis Pentose phosphate pathway Tricarboxylic acid cycle (TCA cycle) and transition step o Aerobic respiration o Anaerobic respiration o Fermentation Part B: Now label on each diagram how the harvested energy is stored during each catabolic process. ATP • Substrate-level phosphorylation and/or • Oxidative phosphorylation Proton motive force • Electron transport chain o Oxidation-reduction (redox) reactions o Active transport o Proton (H+) concentration gradient Reducing power • Electron carriers o NADH, FADH2, NADPH Part C: Make precursor metabolites and their subunits. Now for the last part of catabolism, we need to understand that precursor metabolites are made by the central metabolic pathways and are used to make subunits. Label on your diagram where you would expect precursor metabolites to be made.