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Decomposition Prof. Dr. Asrar M. Khan Decomposition • In biology, decomposition refers to the reduction of the body of a formerly living organism into simpler forms of matter. • Decomposition or rotting is the process by which tissues of a dead organism break down into simpler forms of matter. The process is essential for new growth and development of living organisms because it recycles the finite matter that occupies physical space in the biome. • Bodies of living organisms begin to decompose shortly after death. It is a cascade of processes that go through distinct phases. It may be categorized in two stages by the types of end products. Decomposition • It may be categorised in two stages by the types of end products. The first stage is characterized by the formation of liquid materials; flesh or plant matter begin to decompose. • The second stage is limited to the production of vapors. • The science which studies such decomposition generally is called taphonomy from the Greek word taphos, which means grave. • Historically the progression of decomposition of the flesh of dead organisms has been viewed also as four phases: Decomposition • • • • • fresh (autolysis), bloat (putrefaction), decay (putrefaction and carnivores) and dry (diagenesis). In biology autolysis or more commonly known as self digestion refers to the destruction of a cell through the action of its own enzymes. It may also refer to the digestion of an enzyme by another molecule of the same enzyme. The term derives from the Greek words αυτό ("self") and λύσις ("splitting"). Decomposition • Autolytic cell destruction is uncommon in living adult organisms and usually occurs in injured cells or dying tissue. • Autolysis is initiated by the cells' lysosomes releasing the digestive enzymes they contain out into the cytoplasm. • The cell then, in effect, starts to digest itself. Autolysis of individual cell organelles can be lessened if the organelle is stored in ice-cold isotonic buffer after cell fractionation. • Use • Because primate fetuses grow webbed fingers and toes, autolysis breaks down the webbing as they develop. • Bread baking, winemaking etc. Decomposition • Lysosomes are spherical organelles that contain enzymes (acid hydrolases). They break up food so it is easier to digest. They are found in animal cells, while in yeast and plants the same roles are performed by lytic vacuoles. • Some important enzymes found within lysosomes include: • Lipase digests lipids, • Amylase digests carbohydrates, sugar • Protease digests protein • Nuclease digests nucleic acid and Phoshoric acid monoesters • Lysosomes are the cells' garbage disposal system. They are used for the digestion of macromolecules from phagocytosis (ingestion of other dying cells or larger extracellular material, like foreign invading microbes), Decomposition • Decomposition of plant matter occurs in many stages. It begins with leaching by water; the most easily lost and soluble carbon compounds are liberated in this process. Another early process is physical breakup or fragmentation of the plant material into smaller bits which have greater surface area for microbial colonization and attack. • Decomposition begins at the moment of death, caused by two factors: autolysis, the breaking down of tissues by the body's own internal chemicals and enzymes, and putrefaction, the breakdown of tissues by bacteria. These processes release gases that are the chief source of the unmistakably putrid odor of decaying animal tissue. Decomposition • Humus is a complex organic substance resulting from the breakdown of plant material in a process called humification. This process can occur naturally in soil, or in the production of compost. • Humus is extremely important to the fertility of soils in both a physical and chemical sense. • In soil science, humus refers to any organic matter that has reached a point of stability, where it will break down no further and might, if conditions do not change, remain essentially as it is for centuries, if not millennia. Decomposition • The rate and manner of decomposition in an animal body is strongly affected by a number of factors. In roughly descending degrees of importance, they are: • Temperature, The availability of oxygen, Prior embalming, Cause of death, • Burial, and depth of burial, Access by scavengers,Trauma, including wounds and crushing blows, Humidity, or wetness, Rainfall • Body size and weight, Clothing, The surface on which the body rests • Foods/objects inside the specimen's digestive tract