* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Safety - Chemical & Biomolecular Engineering
Nucleic acid analogue wikipedia , lookup
Bioinformatics wikipedia , lookup
Gene nomenclature wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Gene expression profiling wikipedia , lookup
Point mutation wikipedia , lookup
Gene expression wikipedia , lookup
Genome editing wikipedia , lookup
Gene therapy wikipedia , lookup
Real-time polymerase chain reaction wikipedia , lookup
DNA vaccination wikipedia , lookup
Endogenous retrovirus wikipedia , lookup
Gene regulatory network wikipedia , lookup
Gene prediction wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Community fingerprinting wikipedia , lookup
Molecular cloning wikipedia , lookup
Silencer (genetics) wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Designer baby wikipedia , lookup
Genetic engineering wikipedia , lookup
Biotechnology wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Biomolecular Engineering Concentration Did you know… Each cell contains approximately 9 feet of DNA. In a typical meal, you eat approximately 55,000,000 cells, or about 93,205 miles of DNA! As of 2005, 59% of Europeans believed that tomatoes, and for that sake plants in general, do not contain DNA. Public misconceptions of molecular biotechnology and genetic engineering (GE) Then, what is biotechnology about? •Pharmaceuticals/Medicine (insulin, gene therapy, diagnostics) •Agriculture (improved plants and crops) •Environmental sciences (Bioremediation) •Food products (sweetener for soft drinks and candy) •Forensics (DNA fingerprinting) •Textiles (stone washed jeans) •Basic research Cloning Genes: Recombinant Human Insulin 1978: Scientists at Genentech cloned the gene for human insulin. Genentech licensed the technology to Eli Lilly, where it was named "Humulin”. 1982: It became the first recombinant DNA drug approved by FDA. transformation Cloning and Recombinant Expression of the Human Insulin Gene Protein engineering of laundry detergent Subtilisin • protease that digests proteins that stain clothing; used as an additive to laundry detergent • the gene encoding it was cloned and subtilisin is made recombinantly in E. coli Problem • Inactivated by bleach due to oxidation of methionine at position 22 Solution • Using site directed mutagenesis, mutate methionine 22 to alanine to create a subtilisin enzyme that is stable and active in bleach Flavonoid Genes in Petunia: Addition of a Limited Number of Gene Copies May Lead to a Suppression of Gene Expression A. R. van der Krol, L. A. Mur, M. Beld, JNM. Mol and A. R. Stuitje THE PLANT CELL, Vol 2, Issue 4 291-299, 1990 Why is RNA interference of interest? • Basic science: new pathway of gene regulation • Applied science: - Powerful tool for reverse genetics (knockdown gene and see effect), to determine gene function, and to perform pathway analysis • Therapeutic uses: treatment potential for any disease where decreasing a transcript would be beneficial (any disease where your body makes *too much* of something) e.g., HIV, Hepatitis B and C viruses, influenza A virus, cancer, respiratory syncytial viral infection, neurodegenerative diseases, such as age-related macular degeneration (AMD), Huntington’s disease Interested in Molecular Biotechnology? • Take BIT 410 - Core Technologies (Organic Chem and Biosci 183, pre-reqs • Take course in Biochemistry (BCH 451) • Minor in biotechnology at NCSU – www.ncsu.edu/biotechnology • Consider biomolecular engineering concentration (CBE 551) or other life science-related concentrations/courses (e.g., BEC, Bioprocessing) • Do undergraduate research or get summer internship! BIOTECHNOLOGY MINOR BIT Undergraduate Minor Manipulation of Recombinant DNA (BIT 410) General cloning techniques 2 advanced modules Molecular Biotechnology research internship Ethics course http://www.ncsu.edu/biotechnology Cloning the green fluorescent protein (BIT 410) Clontech Jellyfish E. coli pure protein ADVANCED MODULES •Fermentation of recombinant microorganisms •Animal cell culture •Real Time PCR •RNA purification and analysis •Plant tissue culture and transformation •Protein purification •Computer analysis of DNA sequences •DNA Microarrays •RNAi •Genetic engineering of eukaryotic microorganisms •Genome mapping •New modules always under development TEACHING PHILOSOPHY •Active learning •Problem solving •Hands-on experience •Collaboration •Responsible use of genetic technology STATE-OF-THE-ART LABS CAMPUS-WIDE Biochemistry Biological Sciences Chemical engineering Chemistry Civil Engineering Crop Science Entomology Fisheries and wildlife sciences Food science Horticultural Sciences Immunology Microbiology Nutrition Plant Biology School of Management Toxicology Veterinary sciences Wood and Paper Science Zoology